Published online before print January 27, 2003, doi: 10.1161/01.HYP.0000052833.20759.64
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Hypertension. 2003;41:341.)
© 2003 American Heart Association, Inc.
Scientific Contributions |
Antioxidant-Rich Diet Relieves Hypertension and Reduces Renal Immune Infiltration in Spontaneously Hypertensive Rats
From Renal Service and Laboratory, Hospital Universitario, Instituto de Investigaciones Biomédicas (Fundacite-Zulia), Universidad del Zulia (B.R.-I., Y.Q.), Maracaibo, Venezuela; and the Division of Nephrology and Hypertension, Department of Medicine, Physiology and Biophysics, University of California (C.-D.Z., R.K.S., N.D.V.), Irvine.
Correspondence to Dr Bernardo Rodríguez-Iturbe, Renal Service and Laboratory, Hospital Universitario, Instituto de Investigaciones Biomédicas (Fundacite-Zulia), Universidad del Zulia, Maracaibo, Venezuela. E-mail bri{at}iamnet.com
Previous studies have demonstrated that oxidative stress contributes to hypertension and treatments with either antioxidant or immunosuppressive/anti-inflammatory agents improve hypertension in spontaneously hypertensive rats (SHR). The present study was performed to determine if the antihypertensive effects of an antioxidant-rich diet are associated with reduction in the renal immune infiltration. Rats were divided into experimental groups (n=5 each) that were followed 7 months after birth, during which they were fed either a regular or antioxidant-enriched (test) diet as follows: SHR-R group=regular diet; SHR-T group=test diet throughout the experiment; SHR-S group=test diet for 4 months switched to regular diet thereafter; WKY group=control rats given regular diet. The SHR-T rats showed a significant reduction in systolic blood pressure (mm Hg): SHR-T=179.6±12.9 versus SHR-R=207.5±9.6 (P<0.001) and plasma hydrogen peroxide concentration (SHR-T=15±4 µmol/L versus 34±9 in SHR-R rats). This was accompanied by significant reductions of renal tissue nitrotyrosine abundance, tubulointerstitial infiltration (cells/mm2) of lymphocytes (SHR-T=18±3 versus SHR-R=30±4, P<0.001), macrophages (SHR-T= 17±3 versus SHR-R=22±3), and angiotensin II–positive cells (SHR-T= 17±2 versus SHR-R=25±5, P<0.01). Results in the SHR-S group were intermediate between the SHR-R and SHR-T groups. The intensity of the infiltration of lymphocytes, macrophages, and angiotensin II–positive cells significantly correlated with systolic blood pressure. Thus, the present study demonstrates that an antioxidant-enriched diet reduces the renal interstitial inflammation and improves hypertension in SHR. These findings point to interrelation between oxidative stress and inflammatory reactivity in the pathogenesis of hypertension.
Key Words: antioxidants • lymphocytes • macrophages • immune systems • kidney • rats, spontaneously hypertensive
This article has been cited by other articles:
 |
|
 |
|
  H. Drobiova, M. Thomson, K. Al-Qattan, R. Peltonen-Shalaby, Z. Al-Amin, and M. Ali Garlic Increases Antioxidant Levels in Diabetic and Hypertensive Rats Determined by a Modified Peroxidase Method Evid. Based Complement. Altern. Med., February 20, 2009; (2009) nep011v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Koeners, B. Braam, D. M. van der Giezen, R. Goldschmeding, and J. A. Joles A perinatal nitric oxide donor increases renal vascular resistance and ameliorates hypertension and glomerular injury in adult fawn-hooded hypertensive rats Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1847 - R1855. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Grossman Does Increased Oxidative Stress Cause Hypertension? Diabetes Care, February 1, 2008; 31(Supplement_2): S185 - S189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Tian, R. S. Moore, S. Braddy, R. A. Rose, J.-W. Gu, M. D. Hughson, and R. D. Manning Jr. Interactions between oxidative stress and inflammation in salt-sensitive hypertension Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3388 - H3395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Sullivan, L. Semprun-Prieto, E. I. Boesen, D. M. Pollock, and J. S. Pollock Sex and sex hormones influence the development of albuminuria and renal macrophage infiltration in spontaneously hypertensive rats Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1573 - R1579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Bravo, Y. Quiroz, A. Ferrebuz, N. D. Vaziri, and B. Rodriguez-Iturbe Mycophenolate mofetil administration reduces renal inflammation, oxidative stress, and arterial pressure in rats with lead-induced hypertension Am J Physiol Renal Physiol, August 1, 2007; 293(2): F616 - F623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Franco, F. Martinez, Y. Quiroz, O. Galicia, R. Bautista, R. J. Johnson, and B. Rodriguez-Iturbe Renal angiotensin II concentration and interstitial infiltration of immune cells are correlated with blood pressure levels in salt-sensitive hypertension Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R251 - R256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wang, I. Armando, L. D. Asico, C. Escano, X. Wang, Q. Lu, R. A. Felder, C. G. Schnackenberg, D. R. Sibley, G. M. Eisner, et al. The elevated blood pressure of human GRK4{gamma} A142V transgenic mice is not associated with increased ROS production Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2083 - H2092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Hayden, N. A. Chowdhury, S. A. Cooper, A. Whaley-Connell, J. Habibi, L. Witte, C. Wiedmeyer, C. M. Manrique, G. Lastra, C. Ferrario, et al. Proximal tubule microvilli remodeling and albuminuria in the Ren2 transgenic rat Am J Physiol Renal Physiol, February 1, 2007; 292(2): F861 - F867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pacher, J. S. Beckman, and L. Liaudet Nitric Oxide and Peroxynitrite in Health and Disease Physiol Rev, January 1, 2007; 87(1): 315 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Rodriguez-Iturbe, L. Sepassi, Y. Quiroz, Z. Ni, and N. D. Vaziri Association of mitochondrial SOD deficiency with salt-sensitive hypertension and accelerated renal senescence J Appl Physiol, January 1, 2007; 102(1): 255 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Herrera, A. Ferrebuz, E. G. MacGregor, and B. Rodriguez-Iturbe Mycophenolate Mofetil Treatment Improves Hypertension in Patients with Psoriasis and Rheumatoid Arthritis J. Am. Soc. Nephrol., December 1, 2006; 17(12_suppl_3): S218 - S225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. P. Kodavanti, M. C. Schladweiler, A. D. Ledbetter, R. V. Ortuno, M. Suffia, P. Evansky, J. H. Richards, R. H. Jaskot, R. Thomas, E. Karoly, et al. The Spontaneously Hypertensive Rat: An Experimental Model of Sulfur Dioxide-Induced Airways Disease Toxicol. Sci., November 1, 2006; 94(1): 193 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Miguel, I. Recio, M. Ramos, M. A. Delgado, and M. A. Aleixandre Antihypertensive Effect of Peptides Obtained from Enterococcus faecalis-Fermented Milk in Rats. J Dairy Sci, September 1, 2006; 89(9): 3352 - 3359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Rodriguez-Iturbe and R. J. Johnson Role of inflammatory cells in the kidney in the induction and maintenance of hypertension Nephrol. Dial. Transplant., February 1, 2006; 21(2): 260 - 263. [Full Text] [PDF]
|
|
|
|
 |
|
 B. Rodriguez-Iturbe, A. Ferrebuz, V. Vanegas, Y. Quiroz, S. Mezzano, and N. D. Vaziri Early and Sustained Inhibition of Nuclear Factor-{kappa}B Prevents Hypertension in Spontaneously Hypertensive Rats J. Pharmacol. Exp. Ther., October 1, 2005; 315(1): 51 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wilcox Oxidative stress and nitric oxide deficiency in the kidney: a critical link to hypertension? Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R913 - R935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Racasan, B. Braam, H. A. Koomans, and J. A. Joles Programming blood pressure in adult SHR by shifting perinatal balance of NO and reactive oxygen species toward NO: the inverted Barker phenomenon Am J Physiol Renal Physiol, April 1, 2005; 288(4): F626 - F636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sugita, H. Sugita, and M. Kaneki Increased Insulin Receptor Substrate 1 Serine Phosphorylation and Stress-Activated Protein Kinase/c-Jun N-Terminal Kinase Activation Associated With Vascular Insulin Resistance in Spontaneously Hypertensive Rats Hypertension, October 1, 2004; 44(4): 484 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Touyz Reactive Oxygen Species, Vascular Oxidative Stress, and Redox Signaling in Hypertension: What Is the Clinical Significance? Hypertension, September 1, 2004; 44(3): 248 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Chade, M. D. Bentley, X. Zhu, M. Rodriguez-Porcel, S. Niemeyer, B. Amores-Arriaga, C. Napoli, E. L. Ritman, A. Lerman, and L. O. Lerman Antioxidant Intervention Prevents Renal Neovascularization in Hypercholesterolemic Pigs J. Am. Soc. Nephrol., July 1, 2004; 15(7): 1816 - 1825. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Rodriguez-Iturbe, N. D. Vaziri, J. Herrera-Acosta, and R. J. Johnson Oxidative stress, renal infiltration of immune cells, and salt-sensitive hypertension: all for one and one for all Am J Physiol Renal Physiol, April 1, 2004; 286(4): F606 - F616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Taniyama and K. K. Griendling Reactive Oxygen Species in the Vasculature: Molecular and Cellular Mechanisms Hypertension, December 1, 2003; 42(6): 1075 - 1081. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Ritz and V. Haxsen Angiotensin II and Oxidative Stress: An Unholy Alliance J. Am. Soc. Nephrol., November 1, 2003; 14(11): 2985 - 2987. [Full Text] [PDF]
|
|