Published online before print February 10, 2003, doi: 10.1161/01.HYP.0000056768.03657.B4
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Hypertension. 2003;41:592.)
© 2003 American Heart Association, Inc.
Scientific Contributions |
Enhancement of Intrarenal Angiotensinogen in Dahl Salt-Sensitive Rats on High Salt Diet
From the Department of Physiology, Tulane University Health Sciences Center (H.K., L.G.N.), New Orleans, La; and the Department of Pharmacology, Kagawa Medical University (A.N., Y.A.), Kagawa, Japan.
Correspondence to Hiroyuki Kobori, MD, PhD, Department of Physiology, Tulane University Health Sciences Center, 1430 Tulane Ave, SL39, New Orleans, LA 70112-2699. E-mail hkobori{at}tulane.edu
This study was performed to examine whether there is an inappropriate regulation of intrarenal angiotensinogen in Dahl-salt sensitive rats (DS) fed a high salt diet (HS). Dahl salt-resistant rats (DR) and DS were maintained on HS (8% NaCl) or low salt diet (LS, 0.3% NaCl) for 4 weeks. Systolic blood pressure (SBP), measured by tail-cuff plethysmography, was unaltered in DR (DR+HS, 127±3 mm Hg, n=5; DR+LS, 126±3, n=5); however, SBP was significantly increased in DS+HS (208±7, n=9) compared with DS+LS (134±2, n=5). HS suppressed plasma renin activity in both strains (0.7±0.2 ng of angiotensin I/mL per hour in DS+HS, 3.1±0.5 in DS+LS, 0.8±0.2 in DR+HS, 5.1±0.7 in DR+LS). Plasma angiotensinogen levels, measured by Western blot analysis, were also suppressed by HS in both strains (36 919±2170 integrated densitometric unit in DS+HS, 53 028±2752 in DS+LS, 44 722±1721 in DR+HS, 55782±3785 in DR+LS). However, kidney angiotensinogen levels were significantly increased in DS+HS (75 850±4171, integrated densitometric unit) compared with DS+LS (47 232±3470), DR+HS (44 748±8236), and DR+LS (42 504±4052). Urinary excretion of angiotensinogen, measured by radioimmunoassay of angiotensin I after incubation with excess renin, had a similar profile. Urinary excretion of angiotensinogen was significantly increased in DS+HS (2958±531 pmol/d) compared with DS+LS (56±4), DR+HS (31±12), and DR+LS (21±7). These data indicate that intrarenal angiotensinogen is enhanced in DS+HS, which is reflected by the increased urinary excretion of angiotensinogen. The results suggest that DS on HS have an inappropriate augmentation of intrarenal angiotensinogen, which may contribute to impaired sodium excretion during a high salt diet and the development of hypertension in this strain.
Key Words: angiotensin II • angiotensinogen • rats, Dahl • kidney • urine • hypertension, sodium-dependent • Western blot
This article has been cited by other articles:
 |
|
 |
|
  W. Zheng, H. Ji, C. Maric, X. Wu, and K. Sandberg Effect of dietary sodium on estrogen regulation of blood pressure in Dahl salt-sensitive rats Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1508 - H1513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Tian, A. S. Greene, K. Usa, I. R. Matus, J. Bauwens, J. L. Pietrusz, A. W. Cowley Jr, and M. Liang Renal Regional Proteomes in Young Dahl Salt-Sensitive Rats Hypertension, April 1, 2008; 51(4): 899 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Jaimes, M.-S. Zhou, D. D. Pearse, L. Puzis, and L. Raij Upregulation of cortical COX-2 in salt-sensitive hypertension: role of angiotensin II and reactive oxygen species Am J Physiol Renal Physiol, February 1, 2008; 294(2): F385 - F392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tian, R. S. Moore, S. Braddy, R. A. Rose, J.-W. Gu, M. D. Hughson, and R. D. Manning Jr. Interactions between oxidative stress and inflammation in salt-sensitive hypertension Am J Physiol Heart Circ Physiol, December 1, 2007; 293(6): H3388 - H3395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Korshunov, M. Daul, M. P. Massett, and B. C. Berk Axl Mediates Vascular Remodeling Induced by Deoxycorticosterone Acetate Salt Hypertension Hypertension, December 1, 2007; 50(6): 1057 - 1062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kobori, M. Nangaku, L. G. Navar, and A. Nishiyama The Intrarenal Renin-Angiotensin System: From Physiology to the Pathobiology of Hypertension and Kidney Disease Pharmacol. Rev., September 1, 2007; 59(3): 251 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Grigore, N. B. Ojeda, E. B. Robertson, A. S. Dawson, C. A. Huffman, E. A. Bourassa, R. C. Speth, K. B. Brosnihan, and B. T. Alexander Placental insufficiency results in temporal alterations in the renin angiotensin system in male hypertensive growth restricted offspring Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R804 - R811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Franco, F. Martinez, Y. Quiroz, O. Galicia, R. Bautista, R. J. Johnson, and B. Rodriguez-Iturbe Renal angiotensin II concentration and interstitial infiltration of immune cells are correlated with blood pressure levels in salt-sensitive hypertension Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2007; 293(1): R251 - R256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Mori, P. M. O'Connor, M. Abe, and A. W. Cowley Jr Enhanced Superoxide Production in Renal Outer Medulla of Dahl Salt-Sensitive Rats Reduces Nitric Oxide Tubular-Vascular Cross-Talk Hypertension, June 1, 2007; 49(6): 1336 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yamamoto, T. Nakagawa, H. Suzuki, N. Ohashi, H. Fukasawa, Y. Fujigaki, A. Kato, Y. Nakamura, F. Suzuki, and A. Hishida Urinary Angiotensinogen as a Marker of Intrarenal Angiotensin II Activity Associated with Deterioration of Renal Function in Patients with Chronic Kidney Disease J. Am. Soc. Nephrol., May 1, 2007; 18(5): 1558 - 1565. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Lalouel and A. Rohrwasser Genetic Susceptibility to Essential Hypertension: Insight From Angiotensinogen Hypertension, March 1, 2007; 49(3): 597 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-W. Gu, N. Tian, M. Shparago, W. Tan, A. P. Bailey, and R. D. Manning Jr. Renal NF-{kappa}B activation and TNF-{alpha} upregulation correlate with salt-sensitive hypertension in Dahl salt-sensitive rats Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1817 - R1824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, A. F. Chen, and D. H. Wang Enhanced oxidative stress in kidneys of salt-sensitive hypertension: role of sensory nerves Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3136 - H3143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Grote, M. Ortmann, G. Salguero, C. Doerries, U. Landmesser, M. Luchtefeld, R. P. Brandes, W. Gwinner, T. Tschernig, E.-G. Brabant, et al. Critical role for p47phox in renin-angiotensin system activation and blood pressure regulation Cardiovasc Res, August 1, 2006; 71(3): 596 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang and D. H. Wang A Novel Mechanism Contributing to Development of Dahl Salt-Sensitive Hypertension: Role of the Transient Receptor Potential Vanilloid Type 1 Hypertension, March 1, 2006; 47(3): 609 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Rodriguez-Iturbe and R. J. Johnson Role of inflammatory cells in the kidney in the induction and maintenance of hypertension Nephrol. Dial. Transplant., February 1, 2006; 21(2): 260 - 263. [Full Text] [PDF]
|
|
|
|
|
|
B. T. Alexander Fetal programming of hypertension Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R1 - R10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Zhou, I. H. Schulman, P. J. Pagano, E. A. Jaimes, and L. Raij Reduced NAD(P)H Oxidase in Low Renin Hypertension: Link Among Angiotensin II, Atherogenesis, and Blood Pressure Hypertension, January 1, 2006; 47(1): 81 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. B. Farquhar, E. E. Paul, A. V. Prettyman, and M. E. Stillabower Blood pressure and hemodynamic responses to an acute sodium load in humans J Appl Physiol, October 1, 2005; 99(4): 1545 - 1551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kobori, Y. Ozawa, Y. Suzaki, and A. Nishiyama Enhanced Intrarenal Angiotensinogen Contributes to Early Renal Injury in Spontaneously Hypertensive Rats J. Am. Soc. Nephrol., July 1, 2005; 16(7): 2073 - 2080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tian, K. D. Thrasher, P. D. Gundy, M. D. Hughson, and R. D. Manning Jr Antioxidant Treatment Prevents Renal Damage and Dysfunction and Reduces Arterial Pressure in Salt-Sensitive Hypertension Hypertension, May 1, 2005; 45(5): 934 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-X. Wang and P. W. Sanders Mechanism of hypertensive nephropathy in the Dahl/Rapp rat: a primary disorder of vascular smooth muscle Am J Physiol Renal Physiol, January 1, 2005; 288(1): F236 - F242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Vehaskari, T. Stewart, D. Lafont, C. Soyez, D. Seth, and J. Manning Kidney angiotensin and angiotensin receptor expression in prenatally programmed hypertension Am J Physiol Renal Physiol, August 1, 2004; 287(2): F262 - F267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Zhou, E. A. Jaimes, and L. Raij Atorvastatin Prevents End-Organ Injury in Salt-Sensitive Hypertension: Role of eNOS and Oxidant Stress Hypertension, August 1, 2004; 44(2): 186 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Jones Dietary Sodium and Blood Pressure Hypertension, May 1, 2004; 43(5): 932 - 935. [Full Text] [PDF]
|
|
|
|
|
|
H. Kobori, M. C. Prieto-Carrasquero, Y. Ozawa, and L. G. Navar AT1 Receptor Mediated Augmentation of Intrarenal Angiotensinogen in Angiotensin II-Dependent Hypertension Hypertension, May 1, 2004; 43(5): 1126 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Hoagland, A. K. Flasch, A. J. Dahly-Vernon, E. A. dos Santos, M. A. Knepper, and R. J. Roman Elevated BSC-1 and ROMK Expression in Dahl Salt-Sensitive Rat Kidneys Hypertension, April 1, 2004; 43(4): 860 - 865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Zhou, A. G. Adam, E. A. Jaimes, and L. Raij In Salt-Sensitive Hypertension, Increased Superoxide Production Is Linked to Functional Upregulation of Angiotensin II Hypertension, November 1, 2003; 42(5): 945 - 951. [Abstract] [Full Text] [PDF]
|
|