Published online before print September 15, 2003, doi: 10.1161/01.HYP.0000091821.39824.09
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(Hypertension. 2003;42:588.)
© 2003 American Heart Association, Inc.
Scientific Contributions |
Angiotensin II–NAD(P)H Oxidase–Stimulated Superoxide Modifies Tubulovascular Nitric Oxide Cross-Talk in Renal Outer Medulla
From the Department of Physiology, Medical College of Wisconsin, Milwaukee.
Correspondence to Allen W. Cowley, Jr, PhD, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226. E-mail cowley{at}mcw.edu
The source of superoxide (O2·-) production and cell-to-cell interactions of O2·- and nitric oxide (NO) in response to angiotensin II (AngII) were studied by fluorescence microscopic techniques to image rat renal outer medullary microtissue strips. Changes in intracellular O2·- were determined by dihydroethidium-ethidium ratios, and NO was determined with 4,5-diaminofluorescein diacetate. AngII (1 µmol/L) significantly increased O2·- in the isolated, medullary thick ascending limb (mTAL). These responses were inhibited by the superoxide dismutase mimetic 4-hydroxytetramethylpiperidine-1-oxyl (TEMPOL) and by the NAD(P)H oxidase inhibitors diphenylene iodonium and apocynin. AngII did not increase O2·- in either pericytes of isolated, intact vasa recta (VR) or pericytes of VR with a disrupted endothelium, even when surrounded by mTAL. However, AngII did increase O2·- when the tissue strips were preincubated with the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO), indicating that cross-talk of O2·- from mTAL to the VR occurred but was normally inhibited by NO. Also, tissue O2·- reduction by TEMPOL increased the diffusion of NO from mTAL to the pericytes, indicating that cross-talk of NO from the mTAL to the VR is also inhibited by O2·-. We conclude that AngII stimulates O2·- production in mTAL via the NAD(P)H oxidase pathway and that interactions of O2·- and NO ultimately determine the effectiveness of in situ free-radical cross-talk between the mTAL and the VR.
Key Words: free radicals • rats • oxidative stress • renal blood flow • angiotensin II
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