Published online before print February 16, 2004, doi: 10.1161/01.HYP.0000120123.44945.47
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(Hypertension. 2004;43:860.)
© 2004 American Heart Association, Inc.
Scientific Contributions |
Elevated BSC-1 and ROMK Expression in Dahl Salt-Sensitive Rat Kidneys
From Medical College of Wisconsin (K.M.H., A.K.F., A.J.D.-V., E.A.d.S., R.J.R.), Department of Physiology, Milwaukee; Laboratory of Kidney and Electrolyte Metabolism (M.A.K.), National Heart, Lung and Blood Institute, Bethesda, Md.
Correspondence to Richard J. Roman, Medical College of Wisconsin, Department of Physiology, 8701 Watertown Plank Road, Milwaukee, WI 53226. E-mail rroman{at}mcw.edu
This study compared the expression of enzymes and transport and channel proteins involved in the regulation of sodium reabsorption in the kidney of Dahl salt-sensitive (DS) and salt-resistant Brown-Norway (BN) and consomic rats (SS.BN13), in which chromosome 13 from the BN rat has been introgressed into the DS genetic background. The expression of the Na+/K+/2Cl- (BSC-1) cotransporter, Na+/H+ exchanger (NHE3), and Na+-K+-ATPase proteins were similar in the renal cortex of DS, BN, and SS.BN13 rats fed either a low-salt (0.1% NaCl) or a high-salt (8% NaCl) diet. The expression of the BSC-1 and the renal outer medullary K+ channel (ROMK) were higher, whereas the expression of the cytochrome P4504A proteins responsible for the formation of 20-hydroxyeicosatetraenoic (20-HETE) was lower in the outer medulla of the kidney of DS than in BN or SS.BN13 rats fed either a low-salt or a high-salt diet. In addition, the renal formation and excretion of 20-HETE was lower in DS than in BN and SS.BN13 rats. These results suggest that overexpression of ROMK and BSC-1 in the thick ascending limb combined with a deficiency in renal formation of 20-HETE may predispose Dahl S rats fed a high-salt diet to Na+ retention and hypertension.
Key Words: rats, Dahl • Na+, K+-transporting ATPase • sodium • hypertension
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