Published online before print August 30, 2004, doi: 10.1161/01.HYP.0000142893.08655.96
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(Hypertension. 2004;44:405.)
© 2004 American Heart Association, Inc.
Scientific Contributions |
Ovariectomy Augments Hypertension in Aging Female Dahl Salt-Sensitive Rats
From the Department of Anesthesiology (C.H.-L., T.C.), University of Texas Health Science Center, San Antonio, Tex; Center for the Study of Sex Differences in Health, Aging, and Disease (W.Z., H.J., K.S.), Georgetown University, Washington DC; Department of Pharmacology and Toxicology (J.R.H.), Michigan State University, East Lansing.
Correspondence to Carmen Hinojosa-Laborde, PhD, Associate Professor, Department of Anesthesiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900. E-mail laborde{at}uthscsa.edu
The ovariectomized (OVX) Dahl salt-sensitive (DS) rat fed a low-salt diet is a model of postmenopausal hypertension. In addition to estrogen loss, aging can also contribute to postmenopausal hypertension. We hypothesized that: (1) female DS rats on a low-salt diet become hypertensive with age; (2) ovariectomy accelerates age-dependent hypertension in the DS rat caused by estrogen depletion; and (3) this hypertension correlates with increased type 1 angiotensin receptor (AT1R) number (Bmax). Blood pressure was monitored by telemetry from 3 to 12 months and AT1R Bmax was determined by Scatchard analysis in glomeruli and adrenal cortex. Three groups of DS rats were studied: intact, OVX, and 17ß-estradiol–replaced OVX (OVX+E). In intact rats, aging to 12 months resulted in hypertension (159±6 mm Hg) and an 82% decrease in estrogen. Blood pressure in OVX was significantly higher than OVX+E through 12 months of age (173±4 versus 150±8 mm Hg). At 4 months, OVX increased AT1R Bmax compared with intact and OVX+E in both glomeruli and adrenal cortex. Aging also increased AT1R Bmax in these tissues in intact rats. In summary, female DS rats fed a low-salt diet have hypertension develop with age, that is accelerated by OVX and attenuated by estrogen replacement. Concurrently, AT1Rs are upregulated by age and OVX, which is prevented by estrogen replacement. This study suggests that an increased activity of the renin angiotensin system contributes to the development of hypertension, and estrogen protects against this process.
Key Words: aging • Dahl rats • hypertension, sodium-dependent • estrogen • renin-angiotensin system
This article has been cited by other articles:
 |
|
 |
|
  A. Lopez-Ruiz, J. Sartori-Valinotti, L. L. Yanes, R. Iliescu, and J. F. Reckelhoff Sex differences in control of blood pressure: role of oxidative stress in hypertension in females Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H466 - H474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. B. Ojeda, D. Grigore, and B. T. Alexander Developmental Programming of Hypertension: Insight From Animal Models of Nutritional Manipulation Hypertension, July 1, 2008; 52(1): 44 - 50. [Full Text] [PDF]
|
|
|
|
|
|
L. L. Yanes, J. C. Sartori-Valinotti, and J. F. Reckelhoff Sex Steroids and Renal Disease: Lessons From Animal Studies Hypertension, April 1, 2008; 51(4): 976 - 981. [Full Text] [PDF]
|
|
|
|
 |
|
 Md. S. Bhuiyan, N. Shioda, and K. Fukunaga Ovariectomy augments pressure overload-induced hypertrophy associated with changes in Akt and nitric oxide synthase signaling pathways in female rats Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1606 - E1614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Ma. E. D. Esqueda, T. Craig, and C. Hinojosa-Laborde Effect of Ovariectomy on Renal Estrogen Receptor-{alpha} and Estrogen Receptor-{beta} in Young Salt-Sensitive and -Resistant Rats Hypertension, October 1, 2007; 50(4): 768 - 772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. B. Ojeda, D. Grigore, E. B. Robertson, and B. T. Alexander Estrogen Protects Against Increased Blood Pressure in Postpubertal Female Growth Restricted Offspring Hypertension, October 1, 2007; 50(4): 679 - 685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. G. Krause, K. S. Curtis, J. P. Markle, and R. J. Contreras Oestrogen affects the cardiovascular and central responses to isoproterenol of female rats J. Physiol., July 1, 2007; 582(1): 435 - 447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Keck, M. J. Romero-Aleshire, Q. Cai, P. B. Hoyer, and H. L. Brooks Hormonal status affects the progression of STZ-induced diabetes and diabetic renal damage in the VCD mouse model of menopause Am J Physiol Renal Physiol, July 1, 2007; 293(1): F193 - F199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. B. Ojeda, D. Grigore, L. L. Yanes, R. Iliescu, E. B. Robertson, H. Zhang, and B. T. Alexander Testosterone contributes to marked elevations in mean arterial pressure in adult male intrauterine growth restricted offspring Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R758 - R763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Rogers, A. R. Mitchell, C. Maric, K. Sandberg, A. Myers, and S. E. Mulroney Effect of sex hormones on renal estrogen and angiotensin type 1 receptors in female and male rats Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R794 - R799. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Hinojosa-Laborde and M. L. Lindsey Aging Modifies the Cardiac Response to Estrogen: A New Dimension to Hormone Replacement Therapy Hypertension, October 1, 2006; 48(4): 558 - 559. [Full Text] [PDF]
|
|
|
|
|
|
K. Ito, Y. Hirooka, Y. Kimura, Y. Sagara, and K. Sunagawa Ovariectomy Augments Hypertension Through Rho-Kinase Activation in the Brain Stem in Female Spontaneously Hypertensive Rats Hypertension, October 1, 2006; 48(4): 651 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Jazbutyte, K. Hu, P. Kruchten, E. Bey, S. K.G. Maier, K.-H. Fritzemeier, K. Prelle, C. Hegele-Hartung, R. W. Hartmann, L. Neyses, et al. Aging Reduces the Efficacy of Estrogen Substitution to Attenuate Cardiac Hypertrophy in Female Spontaneously Hypertensive Rats Hypertension, October 1, 2006; 48(4): 579 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Arenas, S. J. Armstrong, Y. Xu, and S. T. Davidge Tumor Necrosis Factor-{alpha} and Vascular Angiotensin II in Estrogen-Deficient Rats Hypertension, September 1, 2006; 48(3): 497 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Paul, A. Poyan Mehr, and R. Kreutz Physiology of local Renin-Angiotensin systems. Physiol Rev, July 1, 2006; 86(3): 747 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Song, C. K. Kost Jr., and D. S. Martin Androgens augment renal vascular responses to ANG II in New Zealand genetically hypertensive rats Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2006; 290(6): R1608 - R1615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. H. Schulman, P. Aranda, L. Raij, M. Veronesi, F. J. Aranda, and R. Martin Surgical Menopause Increases Salt Sensitivity of Blood Pressure Hypertension, June 1, 2006; 47(6): 1168 - 1174. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Maric Sex Differences in Cardiovascular Disease and Hypertension: Involvement of the Renin-Angiotensin System Hypertension, September 1, 2005; 46(3): 475 - 476. [Full Text] [PDF]
|
|
|
|
|
|
R. A. Khalil Sex Hormones as Potential Modulators of Vascular Function in Hypertension Hypertension, August 1, 2005; 46(2): 249 - 254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Dean, J. Tan, E. R. O'Brien, and F. H. H. Leenen 17{beta}-Estradiol downregulates tissue angiotensin-converting enzyme and ANG II type 1 receptor in female rats Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2005; 288(3): R759 - R766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Harvey, B. L. Morris, J. A. Miller, and J. S. Floras Estradiol Induces Discordant Angiotensin and Blood Pressure Responses to Orthostasis in Healthy Postmenopausal Women Hypertension, March 1, 2005; 45(3): 399 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Reckelhoff Sex Steroids, Cardiovascular Disease, and Hypertension: Unanswered Questions and Some Speculations Hypertension, February 1, 2005; 45(2): 170 - 174. [Full Text] [PDF]
|
|
|
|
|
|
L. M. Harrison-Bernard Targeting of the Renin-Angiotensin System as an Adjunct to Estrogen Replacement Therapy Hypertension, October 1, 2004; 44(4): 390 - 391. [Full Text] [PDF]
|
|