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(Hypertension. 2007;50:242.)
© 2007 American Heart Association, Inc.

Sixth International Workshop on Structure and Function of the Vascular System

Validation of Renal Oxidative Metabolism Measurement by Positron-Emission Tomography

Laurent Juillard; Sandrine Lemoine; Marc F. Janier; Paul Y. Barthez; Frédéric Bonnefoi; Maurice Laville

From the Département De Néphrologie (L.J., S.L., M.L.), Hôpital Edouard Herriot, Hospices Civils de Lyon, Lyon, France; ANIMAGE-Centre de Recherche Et d’Application en Traitement de l’Image et du Signal (M.F.J.), Unité mixte de Recherche-Centre National de la Recherche Scientifique 5220, Institut National de la Santé et de la Recherche Médicale U630, Hôpital Edouard Herriot, Hospices Civils de Lyon, Lyon, France; Centre D’exploration et de Recherche Médicale par Emission de Positons (M.F.J., F.B.), Hôpital Cardiologique, Bron, France; Ecole Nationale Vétérinaire De Lyon (P.Y.B.), Marcy L’Etoile, Lyon, France; and Institut National de la Santé et de la Recherche Médicale ERI 22 (L.J., S.L., M.L.), Université de Lyon, Lyon, France.

Correspondence to Laurent Juillard, Hôpital Edouard Herriot, Pavillon P, 3 place D’Arsonval, 69437 Lyon Cedex 03, France. E-mail laurent.juillard{at}chu-lyon.fr

Either in research or in clinical practice, the exploration of renal oxidative metabolism is limited by the lack of noninvasive measurement. Positron-emission tomography using carbon-11 acetate may estimate tissue oxidative metabolism by measuring acetate turnover in the Krebs cycle. Although extensively studied in cardiology, this method has never been validated for renal oxidative metabolism measurement. The aim of this study is the validation of acetate turnover compared with the invasive renal oxygen consumption measurement. Renal oxygen consumption and tubular sodium reabsorption were measured invasively in 10 anesthetized pigs. Simultaneously, acetate turnover was estimated by the clearance of carbon-11 acetate in the renal cortex, after a 166-MBq injection of carbon-11 acetate. Renal oxidative metabolism was measured under various conditions induced by mechanical and pharmacological interventions. Renal oxygen consumption and acetate turnover varied on a wide range from 0.05 to 0.29 mmol min^–1 (>5-fold) and from 0.025 to 0.188 minutes^–1 (>7-fold), respectively. Acetate turnover was very significantly correlated with renal oxygen consumption (P<0.0001; R=0.82) and tubular sodium reabsorption (P=0.001; R=0.67). This study demonstrates that acetate turnover measures renal oxidative metabolism noninvasively and quantitatively, consistent with changes in tubular sodium reabsorption. This method may be applied to assess oxidative metabolism in animal models and in humans.

Key Words: cell respiration • kidney • positron-emission tomography • carbon-11 acetate • oxygen consumption