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(Hypertension. 2008;51:474.)
© 2008 American Heart Association, Inc.

Original Articles Part 2

Attenuation of NADPH Oxidase Activation and Glomerular Filtration Barrier Remodeling With Statin Treatment

Adam Whaley-Connell; Javad Habibi; Ravi Nistala; Shawna A. Cooper; Poorna R. Karuparthi; Melvin R. Hayden; Nathan Rehmer; Vincent G. DeMarco; Bradley T. Andresen; Yongzhong Wei; Carlos Ferrario; James R. Sowers

From the Departments of Internal Medicine (A.W.-C., J.H., R.N., S.A.C., P.R.K., M.R.H., N.R., V.G.D., B.T.A., Y.W., J.R.S.) and Medical Pharmacology and Physiology (J.R.S.) and the Diabetes and Cardiovascular Laboratory (A.W.-C., J.H., R.N., S.A.C., P.R.K., M.R.H., N.R., V.G.D., B.T.A., Y.W., J.R.S.), University of Missouri School of Medicine, Columbia; the Harry S. Truman Veterans’ Affairs Medical Center (A.W.-C., J.R.S.), Columbia, Mo; and the School of Medicine (C.F.), Wake Forest University, Winston-Salem, NC.

Correspondence to Adam Whaley-Connell, University of Missouri-Columbia School of Medicine, Department of Internal Medicine, Division of Nephrology, MA436, DC043.0, One Hospital Dr, Columbia, MO 65212. E-mail whaleyconnella{at}health.missouri.edu

Activation of reduced nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase by angiotensin II is integral to the formation of oxidative stress in the vasculature and the kidney. 3-Hydroxy-3-methylglutaryl-coenzyme A reductase inhibition is associated with reductions of oxidative stress in the vasculature and kidney and associated decreases in albuminuria. Effects of 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibition on oxidative stress in the kidney and filtration barrier integrity are poorly understood. To investigate, we used transgenic TG(mRen2)27 (Ren2) rats, which harbor the mouse renin transgene and renin-angiotensin system activation, and an immortalized murine podocyte cell line. We treated young, male Ren2 and Sprague-Dawley rats with rosuvastatin (20 mg/kg IP) or placebo for 21 days. Compared with controls, we observed increases in systolic blood pressure, albuminuria, renal NADPH oxidase activity, and 3-nitrotryosine staining, with reductions in the rosuvastatin-treated Ren2. Structural changes on light and transmission electron microscopy, consistent with periarteriolar fibrosis and podocyte foot-process effacement, were attenuated with statin treatment. Nephrin expression was diminished in the Ren2 kidney and trended to normalize with statin treatment. Angiotensin II–dependent increases in podocyte NADPH oxidase activity and subunit expression (NOX2, NOX4, Rac, and p22^phox) and reactive oxygen species generation were decreased after in vitro statin treatment. These data support a role for increased NADPH oxidase activity and subunit expression with resultant reactive oxygen species formation in the kidney and podocyte. Furthermore, statin attenuation of NADPH oxidase activation and reactive oxygen species formation in the kidney/podocyte seems to play roles in the abrogation of oxidative stress-induced filtration barrier injury and consequent albuminuria.

Key Words: angiotensin II • albuminuria • glomerular filtration barrier • transgenic Ren2 rat • rosuvastatin

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