Published online before print August 31, 2009, doi: 10.1161/HYPERTENSIONAHA.109.137992
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(Hypertension. 2009;54:1062.)
© 2009 American Heart Association, Inc.
Original Articles |
Effect of Epithelial Sodium Channel Blockade on the Myogenic Response of Rat Juxtamedullary Afferent Arterioles
From the Department of Physiology (Z.G., A.K.C., E.W.I.) and the Vascular Biology Center (J.S.P., J.L.H.), Medical College of Georgia, Augusta.
Correspondence to Professor Edward W. Inscho, Department of Physiology, CA3137, Medical College of Georgia, 1120, 15th Street, Augusta, GA 30912. E-mail einscho{at}mail.mcg.edu
The mechanotransduction mechanism underlying the myogenic response is poorly understood, but evidence implicates participation of epithelial sodium channel (ENaC)-like proteins. Therefore, the role of ENaC on the afferent arteriolar myogenic response was investigated in vitro using the blood-perfused juxtamedullary nephron technique. Papillectomy was used to isolate myogenic influences by eliminating tubuloglomerular feedback signals. Autoregulatory responses were assessed by manipulating perfusion pressure in 30-mm Hg steps. Under control conditions, arteriolar diameter increased by 15% from 13.0±1.3 to 14.7±1.2 µm (P<0.05) after reducing perfusion pressure from 100 to 70 mm Hg. Diameter decreased to 11.3±1.1 and 10.6±1.0 µm after increasing pressure to 130 and 160 mm Hg (88±1 and 81±2% of control diameter, P<0.05), respectively. Pressure-mediated autoregulatory responses were significantly inhibited by superfusion of 10 µmol/L amiloride (102±2, 97±4, and 94±3% of control diameter), or 10 µmol/L benzamil (106±5, 100±3, and 103±3% of control diameter), and when perfusing with blood containing 5 µmol/L amiloride (106±2, 97±4, and 97±4% of control diameter). Vasoconstrictor responses to 55 mmol/L KCl were preserved as diameters decreased by 67±4, 55±8, and 60±4% in afferent arterioles superfused with amiloride or benzamil, and perfused with amiloride, respectively. These responses were similar to responses obtained from control afferent arterioles (64±6%, P>0.05). Immunofluorescence revealed expression of the 
, β, and 
subunits of ENaC in freshly isolated preglomerular microvascular smooth muscle cells. These results demonstrate that selective ENaC inhibitors attenuate afferent arteriolar myogenic responses and suggest that ENaC may function as mechanosensitive ion channels initiating pressure-dependent myogenic responses in rat juxtamedullary afferent arterioles.
Key Words: myogenic response • epithelial sodium channel • amiloride • benzamil • juxtamedullary nephrons • autoregulatory response
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