(Hypertension. 1997;30:934-941.)
© 1997 American Heart Association, Inc.
Articles |
Endothelial Dysfunction Coincides With an Enhanced Nitric Oxide Synthase Expression and Superoxide Anion Production
From the Zentrum der Physiologie, Klinikum der J.W. Goethe-Universität (A.B., J.B., I.F., R.B.), and PGU Cardiovascular Agents, Hoechst Marion Roussel (W.L., B.A.S., G.W.), Frankfurt/Main, Germany.
Correspondence to Anne Bouloumié, PhD, Zentrum der Physiologie, Klinikum der J.W. Goethe-Universität, Theodor-Stern-Kai 7, D-60590 Frankfurt/Main, FRG.
 |
Abstract |
|---|
 Top Abstract IntroductionMethodsResultsDiscussionReferences |
|---|
Abstract We investigated the effects of aortic banding–induced hypertension on the endothelium-dependent vasodilator responses in the aorta and coronary circulation of Sprague-Dawley rats. We studied the influence of hypertension on the endothelial nitric oxide synthase (NOS III) expression, assessed by Western blot and reverse transcription–polymerase chain reactions experiments, and on the superoxide anion (O2-) production. Two weeks after aortic banding, the endothelium-dependent relaxations were not altered. At this time, the expression of NOS III in the aorta and in confluent coronary microvascular endothelial cells (RCMECs) exhibited no marked changes, whereas O2- production was enhanced 1.9-fold in aortas from aortic-banded rats. Six weeks after aortic banding, the endothelium-dependent dilations were markedly impaired in the heart (50% decrease) and aorta (35% decrease). Analysis of NOS III protein and mRNA levels revealed marked increases in both aortas and confluent RCMECs (2.6- to 4-fold) from aortic-banded compared with sham-operated rats. There was no further increase in O2- production in both the aorta and confluent RCMECs from aortic-banded rats. An enhanced nitrotyrosine protein level was also detected in the aorta from 6-week aortic-banded rats. These findings indicate that in hypertension induced by aortic banding, an enhanced O2- production alone is not sufficient to produce endothelial dysfunction. Endothelial vasodilator hyporesponsiveness was observed only when NOS III expression and O2- production were increased and was associated with the appearance of enhanced nitrotyrosine residues. This would suggest that the development of endothelial dysfunction is linked to an overproduction of not one, but two, endothelium-derived radicals that might lead to the formation of peroxynitrite.
Key Words: endothelium • nitric oxide synthase • superoxide dismutase • peroxynitrite
|
Introduction |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
The endothelium plays a crucial role in the control of local vascular tone through the production of vasoconstricting and vasodilating autacoids. Among these factors, the most important appears to be NO, which is synthesized from L-arginine by the constitutive NO synthase (NOS III) under basal and stimulated conditions.1 The physiological significance of endothelium-derived NO in regulating cardiovascular homeostasis is highlighted by the findings that systemic administration of NOS inhibitors results in a marked increase in arterial blood pressure2 and that homologous recombination resulting in the lack of the gene encoding NOS III in mice results in significant hypertension.3
In various models of hypertension, impaired endothelium-dependent relaxations have been described, implying that this pathophysiological state is associated with a so-called endothelial dysfunction and an apparent decrease in the production of bioactive NO.4 5 The cellular mechanisms involved in the development of this dysfunction remain to be fully elucidated. In vessels from rats with either genetic4 or angiotensin II–induced6 hypertension, enhanced endothelial superoxide anion (O2-) production has been described. Such an increase in O2- production likely accelerates the inactivation of NO and accounts for the apparent decrease in bioactive NO. Although it is generally accepted that such an effect may explain the blunted vasodilator response observed in various forms of hypertension,7 controversy exists regarding the effect of hypertension on NOS III expression and activity (for review, see Reference 88 ). In the present study, we investigated the effects of experimental hypertension, induced by aortic banding, on the endothelium-dependent dilator responses in the aorta and heart of rats. The changes in vascular reactivity were associated with alterations in O2- production and NOS III expression in native macrovascular and cultured microvascular endothelial cells.
|
Methods |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Materials
Bradykinin was purchased from Bachem, NG-nitro-L-arginine from Serva, and diclofenac (Voltaren injection solution) from CIBA-Geigy. Bovine recombinant SOD (Peroxinorm) was provided by Grünenthal. All other biochemicals were obtained in the highest purity available from Sigma Chemical Co or Merck. The [
-32P]dCTP was
purchased from Hartmann Analytic. The cloned bovine NOS III cDNA was a
gift from D.G. Harrison, Emory University, Atlanta, Ga. The mouse
nitrotyrosine antibody was a gift from J.S. Beckman, University of
Alabama, Birmingham. The mouse NOS III antibody was purchased from
Transduction Laboratories.
Aortic Banding
Male Sprague-Dawley rats weighing 250 to 280 g were
obtained from Möllegaard (Skensved, Denmark). Animals were housed
in a room in which a constant temperature and humidity were maintained.
Rats were fed Altromin and had free access to food and water. Animal
care and treatment were conducted in conformity with institutional
guidelines that are in compliance with German laws and policies. The
rats were fasted for 12 hours before surgery. Anesthesia
was induced by intraperitoneal injection of 200
mg/kg hexobarbital (Evipan). The abdomen was opened by a cut
parallel to the linea alba; the abdominal aorta was exposed above the
left renal artery and a silk thread was passed under it. A cannula
(0.9x40 mm) was placed longitudinally on the aorta, and both the
aorta and cannula were tied. The cannula was then removed, leaving an
aortic lumen determined by the diameter of the cannula. Before the
abdomen was closed with cat gut, the animals received 5.5 mg
rolitetracycline (Reverin, Hoechst AG). The skin was then closed by
clipping and covered with tar spray. Sham-operated animals were
subjected to the same procedure, without aortic banding. During the
first 5 days after surgery, the animals received tetracycline (1
g/350 mL) in the drinking water.
Isolation and Culture of RCMECs
RCMECs were isolated as described by Piper et
al,9 with minor modifications. The animals were
anesthetized with 200 mg/kg hexobarbital IP, and blood
pressure was measured via catheters in the left carotid artery. The
hearts were excised and mounted via the aorta to a Langendorff
perfusion system. After 5 minutes of non-recirculating perfusion
(80 mm Hg; 10 mL/min) with a modified KHB (pH 7.4, 37°C,
continuously gassed with 95% O2 and 5% CO2),
perfusion was switched to the recirculating mode with 40 mL protease
solution (Boehringer) (dispase II from Bacillus
polymyxa, 120 µg/mL; activity, 0.5 IU/mg; trypsin, 120
µg/mL; collagenase D from Clostridium
histolyticum, 30 IU/mL; albumin, 270 µg/mL;
CaCl2, 25 µmol/L) dissolved in KHB. After 15
to 20 minutes, atria were discarded, and the ventricles were minced and
again incubated for 15 minutes in 10 mL of the recirculating protease
solution. The cell homogenate was filtered through nylon
mesh (200 µm). The resulting filtrate was mixed with stock
Percoll (Sigma; density, 1.12 mg/mL), yielding a density of 1.01
g/mL. Isolation of RCMECs was performed by two linear Percoll
gradient centrifugations. The first gradient (density,
0.498 to 0.876 g/mL) provided a separation of muscle and
nonmuscle cells, and by the second gradient (density, 0.168 to 0.62
g/mL), RCMECs were separated from the nonmuscle cell fraction.
The RCMEC fraction was washed once with modified HEPES buffer (pH 7,
in mmol/L: NaCl 140, KCl 5, MgCl2 0.8,
CaCl2 1.8, Na2HPO4 1, HEPES 25, and
glucose 5) and centrifuged at 173g for 10 minutes.
Thereafter, RCMECs were cultured in medium containing a 1:1 formulation
of Dulbecco's modified Eagle's and Ham's F-12 media supplemented
with penicillin (50 IU/mL), streptomycin (50 µg/mL),
L-glutamine (1 mmol/L), glutathione and
l-(+)-ascorbic acid (5 µg/mL each, Biotect protection medium),
heparin (8.8 IU/mL), endothelial cell growth supplement
(50 µg/mL), and heat-inactivated calf serum
(20%). RCMECs were seeded on six-well plates (Nunc Intermed) precoated
with collagen A and were maintained in a 95% O2/5%
CO2 humidified incubator at 37°C. The culture medium was
changed 1 hour after seeding and the following day. Experiments were
performed 1 week after seeding for confluent RCMECs. For extraction of
protein and mRNA, RCMECs were frozen at -80°C in guanidinium
thiocyanate solution.10
Isolated Perfused Rat Heart
Rats were anesthetized with sodium pentobarbital (60
mg/kg IP). After heparinization (500 U IV), the thorax was
opened and the heart rapidly perfused in situ through a cannula
inserted into the aortic stump (Langendorff preparation). After
ligation of the superior and inferior venae cavae close to
the right atrium, the heart was excised and perfused at a constant flow
to obtain a CPP of approximately 75 mm Hg. Modified KHB (pH 7.4,
37°C, continuously gassed with 95% O2 and 5%
CO2) containing the cyclooxygenase
inhibitor diclofenac (1 µmol/L) was used as
perfusate. CPP was monitored with a pressure transducer (Gould
P2310) connected to a sidearm of the aortic perfusion cannula.
Isovolumetric LVP was measured with a fluid-filled latex balloon
inserted into the left ventricle via the left atrium and connected to a
second pressure transducer (Gould CP-01). Balloon volume was adjusted
to obtain a diastolic pressure of about 10 mm Hg;
heart rate was derived from the LVP signal by a cardiotachometer. The
heart was allowed to equilibrate for at least 20 minutes so a stable
CPP was obtained. Vasodilator agents, bradykinin (3 to 300 pmol) or SNP
(1 and 10 nmol), were administered to the coronary vascular bed
as bolus injections (10 µL). Dilator responses were calculated as the
percentage decrease in CPP.
Vascular Reactivity Studies
The descending thoracic aorta was removed from
anesthetized (60 mg/kg sodium pentobarbital IP) rats,
cleaned of connective tissue, and dissected into three sections. The
upper section (15 mm) was immediately frozen in liquid nitrogen
for Western blot analysis. The lower section (10 mm) was
used for measurement of superoxide anion production, and the
remaining middle part was cut into four rings of about 3 mm in
length that were mounted in a thermostated (37°C) organ bath
(Schuler-Organbad, Hugo Sachs Elektronik) for isometric measurement of
contractile tone. The rings were equilibrated for 30 minutes under a
resting tension of 2 g in carbogenated (95% O2, 5%
CO2) KHB, pH 7.4, in the presence of diclofenac (1
µmol/L). Rings were repeatedly contracted by
phenylephrine (1 µmol/L) until reproducible
contractions were obtained. Thereafter, the relaxant response to
acetylcholine was assessed in the presence or absence of SOD (100
nmol/L). After a washout period, rings were contracted by
phenylephrine (1 µmol/L) in the presence of
NG-nitro-L-arginine (a NOS
inhibitor, 100 µmol/L), and the relaxant
response to SNP was assessed.
Analysis of NOS III Expression by RT-PCR
Total RNAs were extracted from confluent RCMECs according to the
method of Chomczynski and Sacchi.10 For the RT step, 2
µg total RNA was incubated with 200 U reverse transcriptase (GIBCO),
dNTP (125 µmol/L), oligo(dT) (200 ng), and reaction
buffer in a final volume of 20 µL at 37°C for 60 minutes. In some
reaction mixtures, reverse transcriptase or total RNA was omitted to
determine the amplification of contaminating genomic DNA or cDNA. After
a final denaturation step at 94°C for 7 minutes, 6 µL cDNA was
subjected to PCR consisting of denaturation at 94°C for 1 minute,
followed by 90 seconds of annealing at 52°C and 90 seconds of
elongation at 72°C for 30 cycles. The last cycle was ended with 7
minutes of elongation at 72°C. In each PCR, the cDNAs for NOS III and
GAPDH were coamplified. The primers used to amplify NOS III were
derived from the sequence of the partially cloned rat NOS III cDNA
(Genbank accession number, U02534) (sense primer:
5'-CGTGCGCCAGGCTCTCACTTAC-3') and from the sequence of the cloned
human NOS III cDNA11 12 (antisense primer:
5'-GGCTGCAGCCCTTTGCTCTCA-3'), allowing the amplification of a
550-bp fragment. The primers used to amplify GAPDH were derived from
the cloned rat GAPDH cDNA13 (sense primer:
5'-TATGACAACTCCCTCAAGAT-3'; antisense primer:
5'-AGATCCACAACGGATACATT-3'), allowing the amplification of a 320-bp
fragment. The PCR contained 0.4 µmol/L of each primer,
dNTP (200 µmol/L), MgCl2 (1
mmol/L) reaction buffer, and 2.5 U Taq polymerase
(Promega) in a final volume of 50 µL. The amplified cDNAs were
size-fractionated by agarose gel electrophoresis, visualized under UV
light with ethidium bromide staining, transferred to a nylon membrane
(Hybond-N), and hybridized with a 32P-labeled NOS III
fragment obtained from the cloned bovine NOS III cDNA and with a
32P-labeled GAPDH fragment isolated by PCR. The NOS III
cDNA and GAPDH cDNA were quantified after
autoradiography by scanning densitometry. The NOS III
cDNA was normalized by comparison with GAPDH cDNA.
Western Blot Analysis
Crude protein extracts were obtained after alcoholic
precipitation of the phenol phase obtained after the guanidinium
isothiocyanate/phenol/chloroform extraction
method.10 A total of 100 µg of the extracts was
subjected to SDS-PAGE and transferred to nitrocellulose membranes
(Bio-Rad) as previously described.14 Prestained molecular
weight marker proteins (Bio-Rad) were used as standards for SDS-PAGE.
Ponceau staining was performed to verify the quality of the transfer
and equal amounts of protein in each lane. Proteins were detected using
their respective antibodies as described in "Results" and were
visualized by enhanced chemiluminescence using a commercially available
kit (Amersham). Autoradiographs were analyzed by scanning
densitometry.
Measurement of Reactive Oxygen Species
The O2- generation of the rings was
assessed by lucigenin-enhanced chemiluminescence as described
previously.15 For measurement of
H2O2, confluent primary cultures of RCMECs on
quartz coverslips were loaded with the fluorescent dye 5- (and
6-)
carboxy-2',7'-dichlorodihydrofluorescein
diacetate (DCHF, Molecular Probes) by incubation with 20
µmol/L DCHF bis(acetoxymethyl) ester at 37°C for 40 minutes.
Thereafter, the coverslips were washed in HEPES-modified Tyrode's
solution of the following composition (mmol/L): NaCl 132, KCl 4,
CaCl2 1, MgCl2 0.5, HEPES 9.5, and glucose 5;
the rate of intracellular H2O2 formation was
determined fluorometrically (Deltascan, Photon Technology Inc) over 30
minutes using an excitation wavelength set at 490 nm and emission
wavelength of 520 nm.
Statistics
Data are expressed as mean±SEM. Statistical analysis
was performed by one-way ANOVA followed by a Bonferroni t
test or two-tailed Student's t test for unpaired data when
appropriate. Values of P<.05 were considered
statistically significant.
|
Results |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Mean Arterial Blood Pressure and Heart Weight
Mean blood pressure and left ventricular weight were significantly enhanced in rats 2 weeks after aortic banding compared with sham-operated rats, whereas right ventricular weight remained unchanged (Table 1
). No further
increase in either blood pressure or ventricular weight was
observed in animals 6 weeks after aortic banding. The weight gain in
aortic-banded animals did not differ significantly from that in the
sham-operated animals. Mean blood pressure in sham-operated animals 2
and 6 weeks after surgery did not differ (Table 1).
|
Vasodilator Responses in Isolated Perfused Heart
No differences in basal CPP, heart rate, and flow were observed
between sham-operated rats and rats subjected to aortic banding
(Ab-rats) (Table 2). LVP was moderately
increased in the hearts from Ab-rats, although the difference failed to
attain statistical significance. Bolus injections of the
endothelium-dependent vasodilator bradykinin (3 to 300
pmol) induced a dose-dependent decrease in CPP in the hearts from
sham-operated rats and Ab-rats (Fig 1A and 1B). Although slightly attenuated, no
significant changes in the amplitude of the bradykinin-induced dilation
were apparent 2 weeks after aortic banding (Fig 1A). However, the
dilator response to bradykinin was markedly reduced in the hearts from
Ab-rats after 6 weeks (Fig 1B), the maximal dilator response being only
50% of that observed in hearts from sham-operated animals (n=5,
P<.05). The endothelium-independent
vasodilator SNP elicited a dose-dependent decrease in CPP without
affecting LVP or heart rate. Two weeks after aortic banding, the
SNP-induced dilation was decreased, although the difference failed to
attain statistical significance (Fig 1A). The decrease in the
SNP-induced maximal dilator response was statistically significant 6
weeks after aortic banding (48% decrease, P<.05) (Fig 1B).
|
|
Vasodilator Responses in Aorta
In phenylephrine-preconstricted aortic rings,
acetylcholine induced a concentration-dependent relaxation that was not
different in rings from sham-operated rats and 2-week Ab-rats (Fig 2A). However, 6 weeks after aortic
banding, the relaxant response to acetylcholine was significantly
blunted (Fig 2B, Table 3). At no time was
a difference observed in the endothelium-independent
relaxation to SNP (Figs 2C and 2D). To determine whether enhanced
production of O2- accounts for the
attenuated vasodilator responses observed in the heart and aorta of
Ab-rats, we studied the acetylcholine-induced relaxation in aortic
rings in the presence of SOD. SOD (100 nmol/L) did not modify
the relaxant response of rings from 2-week Ab-rats (data not shown),
whereas the attenuated relaxation of aortic rings from 6-week Ab-rats
was completely restored (Table 3, n=5).
|
) and aortic-banded
(
) rats 2 (A and C) and 6 (B and D) weeks after aortic banding.
Results are mean±SEM from five separate experiments.
**P<.01 vs sham-operated.
|
Effect of Aortic Banding on Production of Reactive
Oxygen Species
The production of O2- by aortic
rings from Ab-rats, as assessed by lucigenin-enhanced
chemiluminescence, was significantly increased above that observed in
rings from sham-operated rats 2 and 6 weeks after aortic banding (1.9-
and 1.5-fold increase, respectively; P<.05) (Fig 3). O2-
production was already maximal in rings from 2-week Ab-rats. In
the presence of NG-nitro-L-arginine
(100 µmol/L), the O2-
production detected in the aorta from 2- and 6-week Ab-rats was
not significantly modified (data not shown).
|
Since O2- production determines the intracellular H2O2 concentration, we monitored H2O2 production fluorometrically with DCHF in confluent RCMECs isolated from sham-operated rats and Ab-rats. The rate of intracellular H2O2 formation was increased 2.2-fold in RCMECs from 6-week Ab-rats compared with those from sham-operated rats (708±130 versus 329±114 photons per second, respectively; n=4, P<.05).
NOS III Expression in Heart and Aorta
To determine the effect of aortic banding on NOS III expression,
we assessed NOS III protein and mRNA levels in aortic segments and in
RCMECs. No difference in NOS III protein content was detected between
the aortic segments from sham-operated rats and 2-week Ab-rats (Fig 4A). However, 6 weeks after aortic
banding, NOS III expression was enhanced fourfold over the level
detected in aortas from sham-operated animals (n=4, P<.05)
(Fig 4B). No marked change was observed in NOS III expression in
confluent RCMECs from 2-week Ab-rats compared with cells from
sham-operated rats (Fig 5A); NOS III expression was strongly increased
in cells from 6-week Ab-rats (fourfold increase, P<.05)
(Fig 5B). The effects of aortic banding
on NOS III expression in RCMECs were reflected in the steady-state
level of NOS III mRNA. In RT-PCR experiments, performed on the same
cell extracts, the amount of the amplified NOS III cDNA was not
markedly changed in confluent RCMECs from 2-week Ab-rats (Fig 6A) but was strongly increased in cells
from 6-week Ab-rats (2.6-fold increase, P<.05) (Fig 6B).
|
|
|
Effect of Aortic Banding on Peroxynitrite Production
To investigate whether the increase in NOS III expression together
with enhanced O2- production 6 weeks
after aortic banding was associated with peroxynitrite formation and
nitration of tyrosine residues, we performed Western blot
analysis of aortic proteins. The tyrosine nitration of proteins
was taken as an index of peroxynitrite formation in the vascular wall.
Two weeks after aortic banding, a low level of protein tyrosine
nitration was detected, but no difference in the pattern of nitrated
proteins was observed between sham-operated rats and Ab-rats (data not
shown). However, 6 weeks after aortic banding, the tyrosine nitration
of a protein corresponding to approximately 50 kD was found to be
increased twofold over the level observed in the aorta from
sham-operated animals (Fig 7).
|
|
Discussion |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
In the present study, we have demonstrated that the attenuated endothelium-dependent vasodilator responsiveness observed in the aorta and heart from rats with an experimentally induced hypertension is associated with an increased rather than a decreased NOS III expression. Although oxygen-derived free radicals have been proposed to play a crucial role in the development of the endothelial dysfunction, our results show that the enhancement in O2- production alone, which preceded the upregulation of NOS III expression, was not associated with an endothelial vasodilator hyporesponsiveness. The finding that an enhanced tyrosine nitration of proteins was detected in vessels with an apparent endothelial dysfunction suggests that peroxynitrite, the product of the interaction between both NO and O2-, rather than O2- itself might be involved in the impairment of the endothelial-dependent relaxation.
In a departure from the widely held belief that endothelial dysfunction can be attributed to the decreased production of endothelium-derived NO, recent evidence suggests that impaired endothelium-dependent relaxation in hypertension and atherosclerosis is due neither to decreased NOS activity nor to a deficiency in the availability of L-arginine but to an accelerated inactivation of NO by O2-.16 An enhanced vascular O2- production has already been reported in a number of different pathophysiological conditions associated with endothelial dysfunction including hypercholesterolemia17 and various models of hypertension.4 6 This apparent increase in O2- levels may reflect either a reduced O2- scavenging capacity or an enhanced O2- formation. In fact, experimental evidence has been provided to support both hypotheses. In two different experimental rat models of hypertension,18 19 SOD activity has been reported to decrease, a finding that appears to be in contrast with a report of increased SOD protein levels in aortas from rabbits with coarctation-induced hypertension.20 The possibility cannot be excluded that despite enhanced levels of SOD, a decrease in its activity may be one of the major factors contributing to the increased levels of O2- in the vascular wall. In support of the concept that an elevated O2- generation occurs during the development of hypertension, enhanced NADH and NADPH activity has been described in the aortas of rats with an angiotensin II–induced hypertension.6 Since the renin-angiotensin system is rapidly activated after aortic banding,21 22 it is conceivable that an angiotensin II–coupled mechanism may be responsible for the increase in O2- production observed following aortic banding.
Our data suggest that in the early stages of aortic banding–induced hypertension, the endothelium is the source of the increased O2-. Indeed, endothelium removal markedly reduces O2- formation in aortic rings from hypertensive rats (J. Bauersachs, unpublished observations, 1997). A greater amount of O2- production was also found in endothelial cells cultured from spontaneously hypertensive rats.4 An endothelial source of O2- may also account for the observed decrease in SNP responsiveness in the isolated hearts from Ab-rats. NO generated from intracoronary applied SNP would be expected to be partially inactivated in the endothelium before it is able to activate soluble guanylyl cyclase in vascular smooth muscle cells. In contrast in organ bath experiments using aortic rings from Ab-rats, where SNP is able to directly access vascular smooth muscle cells, NO is generated in a compartment that is only marginally within the vascular sphere of influence of endothelium-derived O2- and is therefore less likely to be rapidly inactivated.
Despite an increase in O2- production 2 weeks after aortic banding, endothelium-dependent dilation was not impaired, suggesting that increased levels of O2- alone may not be sufficient to affect vascular responsiveness. The endothelium-dependent dilation was found to be impaired 6 weeks after aortic banding, whereas at this time, a marked upregulation of NOS III expression was observed in the aorta and in RCMECs. An increase in NOS III activity has already been reported in hearts from spontaneously hypertensive rats using techniques that allowed NO detection and measurement of NOS III activity independently of O2- formation.23 24 The cellular mechanisms leading to the enhanced NOS III expression under such conditions remain to be defined. NOS III is, however, known to be upregulated by growth factors and cytokines, such as transforming growth factor-ß25 and basic fibroblast growth factor,26 which are increased in aortas from hypertensive rats.27 Thus, growth factors are potential candidates involved in the upregulation of NOS III expression. NOS III expression is also sensitive to mechanical forces.28 It is therefore likely that not only shear stress but also other hemodynamic determinants, such as distension and pulsatile stretch, might regulate NOS III expression. An unexpected finding was that this enhanced NOS III expression as well as an increased H2O2 production could also be detected in endothelial cells cultured from hearts of 2- and 6-week Ab-rats. This would tend to suggest that hypertension induces a stable and transferable alteration within endothelial cells such as the chronic activation of transcription factors or other mechanisms involved in the regulation of NOS III expression.
Although an enhanced NO formation might be expected to be protective,
we observed that an increase in NOS III expression correlated with a
depression rather than improvement in endothelial
function in aortas from Ab-rats. It has been described that NOS,
particularly NOS I, under conditions of L-arginine and/or
tetrahydrobiopterin depletion,29 30 might generate
O2-. The generation of
O2- by NOS III was also shown to be inhibited
by the NOS inhibitor
N
-nitro-L-arginine methyl
ester.31 Since O2-
production was not increased in aortas from 6-week compared
with 2-week Ab-rats and was not inhibited in the presence of
NG-nitro-L-arginine, it appears that
NOS III is not the source of the O2-
production. Moreover, the NOS III substrate does not seem to be
rate-limiting, as the attenuated endothelium-dependent
dilation in aortas from 6-week Ab-rats was restored in the presence of
SOD.
In vitro, equimolar concentrations of O2- and NO react rapidly to generate the potent oxidant peroxynitrite, which is able to oxidize thiols,32 lipids,33 and desoxyribose34 as well as to nitrate aromatic amino acid residues. The reaction rate for the formation of peroxynitrite is approximately six times faster than the scavenging of O2- by SOD, implying that peroxynitrite formation can occur in vivo.35 Although in certain biological systems bolus administration of peroxynitrite elicits effects that can be attributed to the formation of intermediate NO-releasing compounds,36 more prolonged exposure to peroxynitrite is likely to have deleterious effects, a phenomenon that may be due to depletion of intracellular thiols. Indeed, in the isolated perfused rat heart, high concentrations of peroxynitrite induced a concentration-dependent vasodilator response that was sensitive to oxyhemoglobin and rapidly exhibited tachyphylaxis.37 Repeated administration of peroxynitrite induced vascular dysfunction and attenuated the endothelium-dependent dilation to other vasodilator compounds. How peroxynitrite is able to alter endothelial function is unclear, but it is possible that nitration of tyrosine residues plays a central role in this process. Peroxynitrite-mediated tyrosine nitration of specific proteins attenuates their tyrosine phosphorylation38 and thus is able to inactivate proteins whose activity depends on the phosphorylation of tyrosine residues. For example, agonist-induced calcium signaling in endothelial cells is regulated by tyrosine phosphorylation,14 39 and peroxynitrite is able to inhibit this response.40 As a marked increase in the nitration of a tyrosine-containing protein was detected in aortas from 6-week Ab-rats, it is likely that peroxynitrite was formed in the aorta. However, in-depth studies are required to investigate the involvement of peroxynitrite and subsequent tyrosine nitration in the development of endothelial dysfunction.
In summary, in aortic banding–induced hypertension, impaired endothelium-dependent relaxations were associated with an enhanced O2- production, an upregulation of NOS III expression, and an increase in the nitrotyrosine protein content. Although the evidence is indirect, we suggest that the elevated intracellular levels of O2- and NO react to produce peroxynitrite, which might be involved in the initiation of endothelial dysfunction.
|
Selected Abbreviations and Acronyms |
|---|
|
|
Acknowledgments |
|---|
This study was supported in part by the Deutsche Forschungsgemeinschaft (Bu 43616-1) and the Commission of European Communities (BMH4-CT96-0979). Anne Bouloumié is a recipient of a fellowship from "Association Française pour la Recherche Thérapeutique." The expert technical assistance of Michaela Stächele, Isabel Winter, and Andreas Schäfer is gratefully acknowledged.
Received November 7, 1996; first decision January 3, 1997; accepted March 10, 1997.
|
References |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
1. Busse R, Fleming I. Regulation and functional consequences of endothelial nitric oxide formation. Ann Med. 1995;27:331-340.[Medline] [Order article via Infotrieve]
2.
Rees DD, Palmer RMJ, Moncada S. Role of
endothelium-derived nitric oxide in the regulation of
blood pressure. Proc Natl Acad Sci U S A. 1989;86:3375-3378.
3. Huang PL, Huang Z, Mashimo H, Bloch KD, Moskowitz MA, Bevan JA, Fishman MC. Hypertension in mice lacking the gene for endothelial nitric oxide synthase. Nature. 1995;377:239-242.[Medline] [Order article via Infotrieve]
4.
Grunfeld S, Hamilton CA, Mesaros S, McClain SW,
Dominiczak AF, Bohr DF, Malinski T. Role of superoxide in the
depressed nitric oxide production by the
endothelium of genetically hypertensive rats.
Hypertension. 1995;26:854-857.
5.
Tschudi M, Mesaros S, Lüscher TF, Malinski
T. Direct in situ measurement of nitric oxide in mesenteric
arteries. Hypertension. 1996;27:32-35.
6. Rajagopalan S, Kurz S, Münzel T, Tarpey M, Freeman BA, Griendling KK, Harrison DG. Angiotensin II-mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation. J Clin Invest. 1996;97:1916-1923.[Medline] [Order article via Infotrieve]
7.
Linder L, Kiowski W, Bühler FR, Lüscher
TF. Indirect evidence for release of
endothelium-derived relaxing factor in human forearm
circulation in vivo; blunted response in essential
hypertension. Circulation. 1990;81:1762-1767.
8.
Dominiczak AF, Bohr DF. Nitric oxide and its
putative role in hypertension. Hypertension. 1995;25:1202-1211.
9. Piper HM, Spahr R, Mertens S, Krüzfeld A, Watanabe H. Cell culture techniques in heart and vessel research. In: Piper HM, ed. Microvascular Endothelial Cells From the Heart. New York, NY: Springer-Verlag; 1990:159-177.
10. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156-159.[Medline] [Order article via Infotrieve]
11. Marsden PA, Schappert KT, Chen HS, Flowers M, Sundell CL, Wilcox JN, Lamas S, Michel T. Molecular cloning and characterization of human endothelial nitric oxide synthase. FEBS Lett. 1992;307:287-293.[Medline] [Order article via Infotrieve]
12.
Janssens SP, Shimouchi A, Quertermous T, Bloch DB,
Bloch KD. Cloning and expression of a cDNA encoding human
endothelium-derived relaxing factor/nitric oxide
synthase. J Biol Chem. 1992;267:14519-14522.
13.
Tso JY, Sun XH, Kao TH, Reece KS, Wu R.
Isolation and characterization of rat and human
glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic
complexity and molecular evaluation of the gene. Nucleic
Acids Res. 1985;13:2485-2502.
14.
Fleming I, Fisslthaler B, Busse R. Calcium
signalling in endothelial cells involves activation of
tyrosine kinases and leads to activation of mitogen-activated
protein kinases. Circ Res. 1995;76:522-529.
15. Hecker M, Preiss C, Schini-Kerth VB, Busse R. Antioxidants differentially affect nuclear factor kappa B-mediated nitric oxide synthase expression in vascular smooth muscle cells. FEBS Lett. 1996;380:224-228.[Medline] [Order article via Infotrieve]
16. Fleming I, Busse R. Endothelial dysfunction in atherosclerosis. J Vasc Res. 1996;33:181-194.[Medline] [Order article via Infotrieve]
17. Ohara Y, Peterson TE, Harrison DG. Hypercholesterolemia increases endothelial superoxide anion production. J Clin Invest. 1993;91:2546-2551.
18. Vega GW, Roson MI, Bellver A, Celentano MM, de la Riva IJ. Nitric oxide and superoxide anions in vascular reactivity of renovascular hypertensive rats. Clin Exp Hypertens. 1995;17:817-835.
19. Ito H, Torii M, Suzuki T. Decreased superoxide dismutase activity and increased superoxide anion production in cardiac hypertrophy of spontaneously hypertensive rats. Clin Exp Hypertens. 1995;17:803-816.
20.
Sharma RC, Crawford DW, Kramsch DM, Sevanian A, Jiao
Q. Immunolocalization of native antioxidant scavenger enzymes in
early hypertensive and atherosclerotic arteries: role of oxygen free
radicals. Arterioscler Thromb. 1992;12:403-415.
21. Linz W, Wiemer G, Schaper J, Zimmermann R, Nagasawa K, Gohlke P, Unger T, Schölkens BA. Angiotensin converting enzyme inhibitors, left ventricular hypertrophy and fibrosis. Mol Cell Biochem. 1995;147:89-97.[Medline] [Order article via Infotrieve]
22. Linz W, Schölkens BA, Ganten D. Converting enzyme inhibition specifically prevents the development and induces regression of cardiac hypertrophy in rats. Clin Exp Hypertens A. 1996;11:1325-1350.
23.
Kelm M, Feelisch M, Krebber T, Deussen A, Motz W,
Strauer BE. Role of nitric oxide in the regulation of
coronary vascular tone in hearts from hypertensive
rats. Hypertension. 1995;25:186-193.
24.
Nava E, Noll G, Lüscher TF. Increased
activity of constitutive nitric oxide synthase in cardiac
endothelium in spontaneous hypertension.
Circulation. 1995;91:2310-2313.
25.
Inoue N, Venema RC, Sayegh HS, Ohara Y, Murphy TJ,
Harrison DG. Molecular regulation of the bovine
endothelial cell nitric oxide synthase by transforming
growth factor beta1. Arterioscler Thromb. 1995;15:1255-1261.
26.
Kostyk SK, Kourembanas S, Wheeler EL, Medeiros D,
McQuillan LP, D'Amore PA, Braunhut SJ. Basic fibroblast growth
factor increases nitric oxide synthase production in bovine
endothelial cells. Am J
Physiol. 1995;269:H1583-H1589.
27. Sarzani R, Brecher P, Chobanian AV. Growth factor expression in aorta of normotensive and hypertensive rats. J Clin Invest. 1989;83:1404-1408.
28. Malek AM, Izumo S. Molecular aspects of signal transduction of shear stress in the endothelial cell. J Hypertens. 1994;12:989-999.[Medline] [Order article via Infotrieve]
29.
Pou S, Pou WS, Bredt DS, Snyder SH, Rosen GM.
Generation of superoxide by purified brain nitric oxide
synthase. J Biol Chem. 1992;267:24173-24176.
30. Heinzel B, John M, Klatt P, Bohme E, Mayer B. Ca2+/calmodulin-dependent formation of hydrogen peroxide by brain nitric oxide synthase. Biochem J. 1992;281:627-630.
31.
Pritchard KA, Groszek L, Smalley DM, Sessa WC, Wu M,
Villalon P, Wolin MS, Stemerman MB. Native low-density
lipoprotein increases endothelial cell nitric oxide
synthase generation of superoxide anion. Circ Res. 1995;77:510-518.
32.
Radi R, Beckman JS, Bush KM, Freeman BA.
Peroxynitrite oxidation of sulphydryls. The cytotoxic potential of
superoxide and nitric oxide. J Biol Chem. 1991;266:4244-4250.
33.
Rubbo H, Radi R, Trujillo M, Telleri R, Kalyanaraman B,
Barnes S, Kirk M, Freeman BA. Nitric oxide regulation of
superoxide and peroxynitrite-dependent lipid peroxidation: formation of
novel nitrogen-containing oxidized lipid derivatives.
J Biol Chem. 1994;269:26066-26075.
34.
Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman
BA. Apparent hydroxyl radical production by
peroxynitrite: implications for endothelial injury from
nitric oxide and superoxide. Proc Natl Acad Sci
U S A. 1990;87:1620-1624.
35.
Beckman JS, Koppenol WH. Nitric oxide,
superoxide, and peroxynitrite: the good, the bad, and the ugly.
Am J Physiol. 1996;271:C1424-C1437.
36.
Moro MA, Darley-Usmar VM, Goodwin DA, Read NG,
Zamora-Pinto R, Feelisch M, Radomski MW, Moncada S. Paradoxical
fate and biological action of peroxynitrite on human
platelets. Proc Natl Acad Sci U S A. 1994;91:6702-6706.
37.
Villa LM, Salas E, Darley-Usmar VM, Radomski MW,
Moncada S. Peroxynitrite induces both vasodilatation and
impaired vascular relaxation in the isolated perfused rat
heart. Proc Natl Acad Sci U S A. 1994;91:12383-12387.
38. Gow AJ, Duran D, Malcom S, Ischiropoulos H. Effects of peroxynitrite-induced protein modifications on tyrosine phosphorylation and degradation. FEBS Lett. 1996;385:63-66.[Medline] [Order article via Infotrieve]
39.
Fleming I, Fisslthaler B, Busse R.
Interdependence of calcium signaling and protein tyrosine
phosphorylation in human endothelial
cells. J Biol Chem. 1996;271:11009-11015.
40.
Elliott SJ. Peroxynitrite modulates
receptor-activated Ca2+ signaling in vascular
endothelial cells. Am J
Physiol. 1996;270:L954-L961.
This article has been cited by other articles:
 |
|
 |
|
  H. Duplain, C. Sartori, P. Dessen, P.-Y. Jayet, M. Schwab, J. Bloch, P. Nicod, and U. Scherrer Stimulation of peroxynitrite catalysis improves insulin sensitivity in high fat diet-fed mice J. Physiol., August 15, 2008; 586(16): 4011 - 4016. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Haurani and P. J. Pagano Adventitial fibroblast reactive oxygen species as autacrine and paracrine mediators of remodeling: Bellwether for vascular disease? Cardiovasc Res, September 1, 2007; 75(4): 679 - 689. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Takaya, K.-i. Hirata, T. Yamashita, M. Shinohara, N. Sasaki, N. Inoue, T. Yada, M. Goto, A. Fukatsu, T. Hayashi, et al. A Specific Role for eNOS-Derived Reactive Oxygen Species in Atherosclerosis Progression Arterioscler. Thromb. Vasc. Biol., July 1, 2007; 27(7): 1632 - 1637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Pacher, J. S. Beckman, and L. Liaudet Nitric Oxide and Peroxynitrite in Health and Disease Physiol Rev, January 1, 2007; 87(1): 315 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Searles Transcriptional and posttranscriptional regulation of endothelial nitric oxide synthase expression Am J Physiol Cell Physiol, November 1, 2006; 291(5): C803 - C816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-S. Tan, C.-Y. Chai, J.-R. Wu, J.-L. Yeh, I.-J. Chen, A.-L. Kwan, A. Y. Jeng, H.-Y. Yang, M.-H. Lee, and Z.-K. Dai Differential Change in Expression of Pulmonary ET-1 and eNOS in Rats After Chronic Left Ventricular Pressure Overload. Experimental Biology and Medicine, June 1, 2006; 231(6): 948 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Chou, C.-Y. Chai, J.-R. Wu, M.-S. Tan, C.-C. Chiu, I.-J. Chen, A. Y. Jeng, C.-I Chang, A.-L. Kwan, and Z.-K. Dai The Effects of Debanding on the Lung Expression of ET-1, eNOS, and cGMP in Rats with Left Ventricular Pressure Overload. Experimental Biology and Medicine, June 1, 2006; 231(6): 954 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-A. Li, L. Guo, R. Asmis, M. Nikolova-Karakashian, and E. J. Smart Scavenger Receptor BI Prevents Nitric Oxide-Induced Cytotoxicity and Endotoxin-Induced Death Circ. Res., April 14, 2006; 98(7): e60 - e65. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ardanaz and P. J. Pagano Hydrogen peroxide as a paracrine vascular mediator: regulation and signaling leading to dysfunction. Experimental Biology and Medicine, March 1, 2006; 231(3): 237 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. E. Lau, J. J. Galligan, D. L. Kreulen, and G. D. Fink Activation of ETB receptors increases superoxide levels in sympathetic ganglia in vivo Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R90 - R95. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Taylor and A. W. Cowley Jr. Effect of renal medullary H2O2 on salt-induced hypertension and renal injury Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1573 - R1579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Y. Kim, G. H. Seol, G. H. Liang, J. A. Kim, and S. H. Suh Na+-K+ pump activation inhibits endothelium-dependent relaxation by activating the forward mode of Na+/Ca2+ exchanger in mouse aorta Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2020 - H2029. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Xiao, M. Chen, Z. Zsengeller, H. Li, L. Kiss, M. Kollai, and C. Szabo Poly(ADP-Ribose) Polymerase Promotes Cardiac Remodeling, Contractile Failure, and Translocation of Apoptosis-Inducing Factor in a Murine Experimental Model of Aortic Banding and Heart Failure J. Pharmacol. Exp. Ther., March 1, 2005; 312(3): 891 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bauersachs and A. Schafer Tetrahydrobiopterin and eNOS dimer/monomer ratio-a clue to eNOS uncoupling in diabetes? Cardiovasc Res, March 1, 2005; 65(4): 768 - 769. [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kevelaitis, A. A. Qureshi, C. Mouas, F. Marotte, S. Kevelaitiene, M. Avkiran, and P. Menasche Na+/H+ exchange inhibition in hypertrophied myocardium subjected to cardioplegic arrest: an effective cardioprotective approach Eur. J. Cardiothorac. Surg., January 1, 2005; 27(1): 111 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Laude, H. Cai, B. Fink, N. Hoch, D. S. Weber, L. McCann, G. Kojda, T. Fukai, H. H. H. W. Schmidt, S. Dikalov, et al. Hemodynamic and biochemical adaptations to vascular smooth muscle overexpression of p22phox in mice Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H7 - H12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. N. Bratz and N. L. Kanagy Nitric oxide synthase-inhibition hypertension is associated with altered endothelial cyclooxygenase function Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2394 - H2401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Zhang, T. W. Hein, W. Wang, M. W. Miller, T. W. Fossum, M. M. McDonald, J. D. Humphrey, and L. Kuo Upregulation of Vascular Arginase in Hypertension Decreases Nitric Oxide-Mediated Dilation of Coronary Arterioles Hypertension, December 1, 2004; 44(6): 935 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Therade-Matharan, E. Laemmel, J. Duranteau, and E. Vicaut Reoxygenation after hypoxia and glucose depletion causes reactive oxygen species production by mitochondria in HUVEC Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1037 - R1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Egi, N. E. Conrad, J. Kwan, C. Schulze, R. Schulz, and S. M. Wildhirt Inhibition of inducible nitric oxide synthase and superoxide production reduces matrix metalloproteinase-9 activity and restores coronary vasomotor function in rat cardiac allografts Eur. J. Cardiothorac. Surg., August 1, 2004; 26(2): 262 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Svatikova, R. Wolk, H. H. Wang, M. E. Otto, K. A. Bybee, R. J. Singh, and V. K. Somers Circulating free nitrotyrosine in obstructive sleep apnea Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2004; 287(2): R284 - R287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Berka, G. Wu, H.-C. Yeh, G. Palmer, and A.-l. Tsai Three Different Oxygen-induced Radical Species in Endothelial Nitric-oxide Synthase Oxygenase Domain under Regulation by L-Arginine and Tetrahydrobiopterin J. Biol. Chem., July 30, 2004; 279(31): 32243 - 32251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Ungvari, A. Csiszar, P. M. Kaminski, M. S. Wolin, and A. Koller Chronic High Pressure-Induced Arterial Oxidative Stress: Involvement of Protein Kinase C-Dependent NAD(P)H Oxidase and Local Renin-Angiotensin System Am. J. Pathol., July 1, 2004; 165(1): 219 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hiyoshi, K. Yayama, M. Takano, and H. Okamoto Stimulation of Cyclic GMP Production via AT2 and B2 Receptors in the Pressure-Overloaded Aorta After Banding Hypertension, June 1, 2004; 43(6): 1258 - 1263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schafer, D. Fraccarollo, P. Tas, I. Schmidt, G. Ertl, and J. Bauersachs Endothelial dysfunction in congestive heart failure: ACE inhibition vs. angiotensin II antagonism Eur J Heart Fail, March 1, 2004; 6(2): 151 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Alp and K. M. Channon Regulation of Endothelial Nitric Oxide Synthase by Tetrahydrobiopterin in Vascular Disease Arterioscler. Thromb. Vasc. Biol., March 1, 2004; 24(3): 413 - 420. [Abstract] [Full Text]
|
|
|
|
|
|
K. Yayama, M. Horii, H. Hiyoshi, M. Takano, H. Okamoto, S. Kagota, and M. Kunitomo Up-Regulation of Angiotensin II Type 2 Receptor in Rat Thoracic Aorta by Pressure-Overload J. Pharmacol. Exp. Ther., February 1, 2004; 308(2): 736 - 743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Seol, S. C. Ahn, J. A. Kim, B. Nilius, and S. H. Suh Inhibition of endothelium-dependent vasorelaxation by extracellular K+: a novel controlling signal for vascular contractility Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H329 - H339. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schafer, D. Fraccarollo, S. K Hildemann, P. Tas, G. Ertl, and J. Bauersachs Addition of the selective aldosterone receptor antagonist eplerenone to ACE inhibition in heart failure: effect on endothelial dysfunction Cardiovasc Res, June 1, 2003; 58(3): 655 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. V. Turko and F. Murad Protein Nitration in Cardiovascular Diseases Pharmacol. Rev., December 1, 2002; 54(4): 619 - 634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Munzel, I. B. Afanas'ev, A. L. Kleschyov, and D. G. Harrison Detection of Superoxide in Vascular Tissue Arterioscler. Thromb. Vasc. Biol., November 1, 2002; 22(11): 1761 - 1768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Guzik, N. E.J. West, R. Pillai, D. P. Taggart, and K. M. Channon Nitric Oxide Modulates Superoxide Release and Peroxynitrite Formation in Human Blood Vessels Hypertension, June 1, 2002; 39(6): 1088 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bauersachs, M. Heck, D. Fraccarollo, S. K. Hildemann, G. Ertl, M. Wehling, and M. Christ Addition of spironolactone to angiotensin-converting enzyme inhibition in heart failure improves endothelial vasomotor dysfunction: Role of vascular superoxide anion formation and endothelial nitric oxide synthase expression J. Am. Coll. Cardiol., January 16, 2002; 39(2): 351 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Wiemer, G. Itter, T. Malinski, and W. Linz Decreased Nitric Oxide Availability in Normotensive and Hypertensive Rats With Failing Hearts After Myocardial Infarction Hypertension, December 1, 2001; 38(6): 1367 - 1371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gori, S. S. Mak, S. Kelly, and J. D. Parker Evidence supporting abnormalities in nitric oxide synthase function induced by nitroglycerin in humans J. Am. Coll. Cardiol., October 1, 2001; 38(4): 1096 - 1101. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Wildhirt, M. Weis, C. Schulze, N. Conrad, S. Pehlivanli, G. Rieder, G. Enders, W. von Scheidt, and B. Reichart Expression of Endomyocardial Nitric Oxide Synthase and Coronary Endothelial Function in Human Cardiac Allografts Circulation, September 18, 2001; 104 (2009): I-336 - I-343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, S. Wang, E. V. Nishanian, A. Del Pilar Cintron, R. A. Wesley, and R. L. Danner Signaling by eNOS through a superoxide-dependent p42/44 mitogen-activated protein kinase pathway Am J Physiol Cell Physiol, August 1, 2001; 281(2): C544 - C554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Katoh, Y. Kurosawa, K. Tanaka, A. Watanabe, H. Doi, and H. Narita Fluvastatin inhibits O2- and ICAM-1 levels in a rat model with aortic remodeling induced by pressure overload Am J Physiol Heart Circ Physiol, August 1, 2001; 281(2): H655 - H660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Grieve, P. A. MacCarthy, N. P. Gall, A. C. Cave, and A. M. Shah Divergent Biological Actions of Coronary Endothelial Nitric Oxide During Progression of Cardiac Hypertrophy Hypertension, August 1, 2001; 38(2): 267 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bauersachs, I. Fleming, D. Fraccarollo, R. Busse, and G. Ertl Prevention of endothelial dysfunction in heart failure by vitamin E: Attenuation of vascular superoxide anion formation and increase in soluble guanylyl cyclase expression Cardiovasc Res, August 1, 2001; 51(2): 344 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Wildhirt, M. Weis, C. Schulze, N. Conrad, S. Pehlivanli, G. Rieder, G. Enders, W. von Scheidt, and B. Reichart Coronary flow reserve and nitric oxide synthases after cardiac transplantation in humans Eur. J. Cardiothorac. Surg., June 1, 2001; 19(6): 840 - 847. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zalba, G. S. Jose, F. J. Beaumont, M. A. Fortuno, A. Fortuno, and J. Diez Polymorphisms and Promoter Overactivity of the p22phox Gene in Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats Circ. Res., February 2, 2001; 88(2): 217 - 222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Linke, N. Schoene, S. Gielen, J.u. Hofer, S. Erbs, G. Schuler, and R. Hambrecht Endothelial dysfunction in patients with chronic heart failure: systemic effects of lower-limb exercise training J. Am. Coll. Cardiol., February 1, 2001; 37(2): 392 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Hamilton, M. J. Brosnan, M. McIntyre, D. Graham, and A. F. Dominiczak Superoxide Excess in Hypertension and Aging : A Common Cause of Endothelial Dysfunction Hypertension, February 1, 2001; 37(2): 529 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. B. O'Donnell and B. A. Freeman Interactions Between Nitric Oxide and Lipid Oxidation Pathways : Implications for Vascular Disease Circ. Res., January 19, 2001; 88(1): 12 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ruijtenbeek, F. A. C. le Noble, G. M. J. Janssen, C. G. A. Kessels, G. E. Fazzi, C. E. Blanco, and J. G. R. De Mey Chronic Hypoxia Stimulates Periarterial Sympathetic Nerve Development in Chicken Embryo Circulation, December 5, 2000; 102(23): 2892 - 2897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Roberts, N. D. Vaziri, X. Q. Wang, and R. J. Barnard Enhanced NO Inactivation and Hypertension Induced by a High-Fat, Refined-Carbohydrate Diet Hypertension, September 1, 2000; 36(3): 423 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.Y. Alexander, M.J. Brosnan, C. A. Hamilton, J. P. Fennell, E. C. Beattie, E. Jardine, D. D. Heistad, and A. F. Dominiczak Gene transfer of endothelial nitric oxide synthase but not Cu/Zn superoxide dismutase restores nitric oxide availability in the SHRSP Cardiovasc Res, August 18, 2000; 47(3): 609 - 617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Brandes, F.-H. Schmitz-Winnenthal, M. Feletou, A. Godecke, P. L. Huang, P. M. Vanhoutte, I. Fleming, and R. Busse An endothelium-derived hyperpolarizing factor distinct from NO and prostacyclin is a major endothelium-dependent vasodilator in resistance vessels of wild-type and endothelial NO synthase knockout mice PNAS, August 15, 2000; 97(17): 9747 - 9752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gorlach, R. P. Brandes, K. Nguyen, M. Amidi, F. Dehghani, and R. Busse A gp91phox Containing NADPH Oxidase Selectively Expressed in Endothelial Cells Is a Major Source of Oxygen Radical Generation in the Arterial Wall Circ. Res., July 7, 2000; 87(1): 26 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zalba, F. J. Beaumont, G. S. Jose, A. Fortuno, M. A. Fortuno, J. C. Etayo, and J. Diez Vascular NADH/NADPH Oxidase Is Involved in Enhanced Superoxide Production in Spontaneously Hypertensive Rats Hypertension, May 1, 2000; 35(5): 1055 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Huang, D. Sun, and A. Koller Shear Stress-Induced Release of Prostaglandin H2 in Arterioles of Hypertensive Rats Hypertension, April 1, 2000; 35(4): 925 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Drummond, H. Cai, M. E. Davis, S. Ramasamy, and D. G. Harrison Transcriptional and Posttranscriptional Regulation of Endothelial Nitric Oxide Synthase Expression by Hydrogen Peroxide Circ. Res., February 18, 2000; 86(3): 347 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-E. Massart, J. Donckier, J. Kyselovic, T. Godfraind, G. R. Heyndrickx, and M. Wibo Carvedilol and Lacidipine Prevent Cardiac Hypertrophy and Endothelin-1 Gene Overexpression After Aortic Banding Hypertension, December 1, 1999; 34(6): 1197 - 1201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Busse and I. Fleming A critical look at cardiovascular translational research Am J Physiol Heart Circ Physiol, November 1, 1999; 277(5): H1655 - H1660. [Full Text] [PDF]
|
|
|
|
|
|
H. Ruetten, U. Zabel, W. Linz, and H. H. H. W. Schmidt Downregulation of Soluble Guanylyl Cyclase in Young and Aging Spontaneously Hypertensive Rats Circ. Res., September 17, 1999; 85(6): 534 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Linz, P. Wohlfart, B. A Scholkens, T. Malinski, and G. Wiemer Interactions among ACE, kinins and NO Cardiovasc Res, August 15, 1999; 43(3): 549 - 561. [Full Text] [PDF]
|
|
|
|
|
|
J. Bauersachs, A. Bouloumie, D. Fraccarollo, K. Hu, R. Busse, and G. Ertl Endothelial Dysfunction in Chronic Myocardial Infarction Despite Increased Vascular Endothelial Nitric Oxide Synthase and Soluble Guanylate Cyclase Expression : Role of Enhanced Vascular Superoxide Production Circulation, July 20, 1999; 100(3): 292 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Welch, A. Tojo, J.-U. Lee, D. G. Kang, C. G. Schnackenberg, and C. S. Wilcox Nitric oxide synthase in the JGA of the SHR: expression and role in tubuloglomerular feedback Am J Physiol Renal Physiol, July 1, 1999; 277(1): F130 - F138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kerr, M. J. Brosnan, M. McIntyre, J. L. Reid, A. F. Dominiczak, and C. A. Hamilton Superoxide Anion Production Is Increased in a Model of Genetic Hypertension : Role of the Endothelium Hypertension, June 1, 1999; 33(6): 1353 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bkaily and P. D'orleans-Juste Cytokine-induced free radicals and their roles in myocardial dysfunctions Cardiovasc Res, June 1, 1999; 42(3): 576 - 577. [Full Text] [PDF]
|
|
|
|
|
|
K. Kusterer, T. Pohl, H.-P. Fortmeyer, W. Marz, H. Scharnagl, A. Oldenburg, S. Angermuller, I. Fleming, K. H. Usadel, and R. Busse Chronic selective hypertriglyceridemia impairs endothelium-dependent vasodilatation in rats Cardiovasc Res, June 1, 1999; 42(3): 783 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Brandes, G. Koddenberg, W. Gwinner, D.-y. Kim, H.-J. Kruse, R. Busse, and A. Mugge Role of Increased Production of Superoxide Anions by NAD(P)H Oxidase and Xanthine Oxidase in Prolonged Endotoxemia Hypertension, May 1, 1999; 33(5): 1243 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Roggensack, Y. Zhang, and S. T. Davidge Evidence for Peroxynitrite Formation in the Vasculature of Women With Preeclampsia Hypertension, January 1, 1999; 33(1): 83 - 89. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Marumo, V. B. Schini-Kerth, R. P. Brandes, and R. Busse Glucocorticoids Inhibit Superoxide Anion Production and p22 Phox mRNA Expression in Human Aortic Smooth Muscle Cells Hypertension, December 1, 1998; 32(6): 1083 - 1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, S. Wang, L. Yan, P. Madara, A. Del Pilar Cintron, R. A. Wesley, and R. L. Danner Superoxide Production and Reactive Oxygen Species Signaling by Endothelial Nitric-oxide Synthase J. Biol. Chem., May 26, 2000; 275(22): 16899 - 16903. [Abstract] [Full Text] [PDF]
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||