(Hypertension. 2000;35:25.)
© 2000 American Heart Association, Inc.
Scientific Contributions |
Kallikrein Gene Delivery Attenuates Myocardial Infarction and Apoptosis After Myocardial Ischemia and Reperfusion
From the Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC.
Correspondence to Julie Chao, PhD, Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425-2211. E-mail chaoj{at}musc.edu
 |
Abstract |
|---|
 Top Abstract IntroductionMethodsResultsDiscussionReferences |
|---|
Abstract—The tissue kallikrein-kinin system is present in the heart, and kinin has been shown to have cardioprotective effects. In this study, we investigated the potential role of tissue kallikrein in myocardial ischemia/reperfusion injury through adenovirus-mediated human kallikrein gene delivery. One week after gene delivery, the rats were subjected to a 30-minute coronary occlusion followed by a 2-hour reperfusion. Kallikrein gene delivery caused significant decreases in the ratio of infarct size to ischemic area at risk (from 69.6% to 44.5%, n=10 and 8, P<0.01) and in the incidence of ventricular fibrillation (from 64.3% to 16.7%, n=14 and 24, P<0.01) compared with the group injected with control adenovirus. Kallikrein gene delivery also attenuated programmed cell death in the ischemic area compared with the control area as assessed with the terminal deoxynucleotidyl transferase–mediated nick end labeling assay (n=6, P<0.01). Icatibant, a specific bradykinin B2 receptor antagonist, abolished these kallikrein-mediated beneficial effects. The expression of human tissue kallikrein mRNA was identified in rat heart, kidney, lung, liver, and adrenal gland. After kallikrein gene delivery, cardiac kinin and cGMP levels were significantly elevated compared with the control (29.6±12.7 versus 6.1±2.1 pg/mg protein, n=7, P<0.01; 1.30±0.06 versus 0.86±0.09 pmol/mg protein, n=5, P<0.05). These results indicate that kallikrein gene delivery protects against myocardial infarction, ventricular arrhythmias, and apoptosis in ischemia/reperfusion injury via kinin-cGMP signal pathway. The successful application of this technology may have potential therapeutic value in the treatment of coronary artery diseases.
Key Words: kallikrein • genes • myocardial infarction • arrhythmia • guanosine • kinins
|
Introduction |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Occlusion of the coronary artery causes cardiomyocyte dysfunction and eventual tissue necrosis. Reperfusion relieves ischemia by providing cells with metabolites and oxygen, thereby preventing extensive tissue damage. Although reperfusion salvages the myocardium, it also initiates a series of events that result in ventricular arrhythmia and accelerate myocardial apoptosis and necrosis.1 This consequence greatly limits the benefits of thrombolytic or angioplastic therapy. It is well known that the inhibition of ACE has cardioprotective effects, such as improvement in cardiac function under the condition of heart failure, attenuation of hypertensive cardiac hypertrophy, reduction in myocardial infarct size, and prevention of reperfusion injury.2 3 4 5 6 ACE is the same enzyme as kininase II, a kinin-degrading enzyme. Because icatibant (Hoe 140), a bradykinin B2 receptor antagonist, abolishes the protective effects of ACE inhibition on cardiac function, these beneficial effects act in part via the kallikrein-kinin system.7 8 9 This notion is further supported by the results of a study that show the administration of bradykinin reduces infarct size.8 Ischemic occlusion/reperfusion can induce apoptosis in the myocardium, whereas inhibition of caspase activity has been shown to attenuate both ischemia/reperfusion injury and apoptosis in cardiomyocytes.10 11 However, the potential role of the tissue kallikrein-kinin system in myocardiocyte apoptosis has not been investigated.
The tissue kallikrein-kinin system components, including tissue kallikrein, kininogen, kinin, and bradykinin B2 receptor, have been identified in the heart.12 13 14 Tissue kallikrein cleaves kininogen substrate to release vasoactive kinin peptide via limited proteolysis.15 16 Kinin is then degraded by kininase I or II. Intact kinins bind to B2 receptors, whereas the metabolites of kinin, such as Des-Arg9-bradykinin and Des-Arg10-Lys-bradykinin, bind to B1 receptors.16 The binding of kinins to their respective receptors activates second messengers in target tissues and triggers biological effects such as vasodilation and vasoconstriction. Through the use of somatic gene delivery approaches, we showed that the expression of recombinant human tissue kallikrein resulted in blood pressure reduction, attenuation of renal damage, and cardiac hypertrophy in genetic and experimentally induced hypertensive rat models.17 18 In this study, we examined the potential roles of the tissue kallikrein-kinin system in ischemia/reperfusion injury through the delivery of the human tissue kallikrein gene and show that adenovirus-mediated kallikrein gene delivery significantly attenuated myocardial infarction and apoptosis after ischemia/reperfusion injury in rats.
|
Methods |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Preparation of Replication-Deficient Adenoviral Vector Ad.CMV-cHK
The adenoviral vector containing human tissue kallikrein cDNA under the control of the cytomegalovirus (CMV) enhancer/promoter was prepared as previously described.17 Adenovirus harboring the ß-galactosidase gene under the control of the CMV enhancer/promoter (Ad.CMV-LacZ) was also prepared as previously described.17
Animal Treatment
Wistar male rats weighing 250 to 280 g (Sprague-Dawley;
Harlan) were used in this study. Rats were housed in an air-conditioned
room with a 12-hour light/dark cycle, received a standard rat chow
(0.4% sodium chloride), and drank tap water. All procedures complied
with the standards for the care and use of animal subjects as stated in
the "Guide for the Care and Use of Laboratory Animals" (Institute
of Laboratory Resources, National Academy of Sciences). One week
before surgery, the rats were randomly divided into 5 groups. The first
group was injected with saline via the jugular vein (control, n=23);
the second group was injected with Ad.CMV-LacZ (n=14), and the
third group was injected with Ad.CMV-cHK (n=24) at a dose of
1x1010 plaque-forming units/rat. The fourth and
fifth groups were injected with Ad.CMV-cHK (n=10) or saline (n=10),
respectively, together with icatibant administration (2 µg/kg) via
the jugular vein at 15 minutes before coronary occlusion.
Surgical Preparation
Rats were anesthetized with sodium pentobarbital (50
mg/kg IP) and then incubated and ventilated with room air through the
use of a respirator (model 683; Harvard Apparatus). A
thoracotomy was performed via the fourth intercostal space, and the
heart was exposed. A 6-0 polypropylene suture (Prolene; Ethicon) was
passed loosely around the left anterior descending coronary
artery near its origin. Once the hemodynamics were
stabilized, coronary occlusion was performed by tightening the
suture loop for 30 minutes. Acute myocardial ischemia was
deemed successful on the basis of regional cyanosis of the myocardial
surface distal to the suture, accompanied by elevation of the ST
segment on ECG. The loop was then loosened and reperfused as identified
on the basis of return of the original color, accompanied by an obvious
ST-segment change. The chest was closed in layers, and the animals were
placed on a heating pad throughout the experimental period.
Hemodynamics and ECG
A microtransducer catheter (SPL-320; Millar Instruments) was
inserted into the femoral artery. An ECG was obtained by subcutaneously
inserting needle electrodes into the limbs. Mean arterial
pressure (MAP) and heart rate (HR) were measured with the use of a
polygraph system (model 7E; Grass Instruments). MAP and ECG were
monitored throughout the experimental period. Ventricular
arrhythmia was quantified according to the Lambeth
Conventions.19 If ventricular fibrillation
(VF) occurred during ischemia and did not resolve spontaneously
within 3 seconds, manual cardioversion was attempted through gentle
palpation of the nonischemic region of the heart. We excluded
infarct-size analysis of rats in which VF persisted for >6
seconds or in which cardioversion was performed >4 times. The
incidences of ventricular tachycardia (VT) and
VF were evaluated as they occurred.
Measurement of Myocardial Infarct Size
After a 120-minute reperfusion period, the loop around the left
anterior descending coronary artery was retightened, and 5%
Evans blue was rapidly injected into the left ventricle to distinguish
the nonischemic area from the area at risk. The heart was then
excised, and the atria, great vessels, and right ventricle were
dissected. The left ventricle was cut into 4 slices transversely from
base to apex. The slices were incubated at 37°C with 4%
triphenyltetrazolium chloride for 30
minutes. Each slice was photographed, and information was downloaded
into the computer. The infarct area (unstained), area at risk
(brick-red stained), and noninfarct area (blue stained) were measured
with use of NIH Image program. The following parameters
were averaged for 4 slices from each heart: (1) infarct size expressed
as a percentage of the area at risk and (2) area at risk expressed as a
percentage of the total area of the slice.
Detection of Apoptosis With In Situ Nuclear
DNA Fragmentation
DNA fragmentation was determined through a terminal
deoxynucleotidyl transferase-mediated dUTP nick end
labeling (TUNEL) assay in deparaffinized 4-µm-thick sections from
tissue blocks.20 This procedure was performed by using an
in situ cell death detection kit according to the manufacturer’s
instructions (Boehringer Mannheim). The sections were then
counterstained with 0.05% light green. Cardiomyocytes in the left
ventricle were analyzed in 
10 separate fields for each tissue
section under a light microscope at a magnification of x400. More than
300 cardiomyocytes in the ischemic area per rat
were counted, and cell numbers from 6 rats per group were averaged.
TUNEL-positive cardiomyocytes in the ischemic
myocardium were scored by an individual who was unaware of
the experimental design and were carefully distinguished from
TUNEL-positive noncardiomyocytes, such as leukocytes,
macrophages, or endothelial cells. The ratio of
TUNEL-positive cardiomyocytes to the total number of
cardiomyocytes was calculated.
Tissue Preparation
At the end of the experiment, blood samples of 6 to 8 rats from
each group were collected through direct cardiac puncture and chilled
at 4°C. These samples were centrifuged at 1000g
for 20 minutes, and sera were collected and frozen at -20°C. At the
same time, rats were perfused with normal saline from the heart, and
the heart was rapidly excised and rinsed in cold normal saline. The
left ventricle was separated from the right ventricle and atria and
then stored at -80°C until further analysis.
Reverse-Transcription Polymerase Chain Reaction Southern
Blot Analysis of Human Tissue Kallikrein mRNA
Total RNA was extracted from fresh rat tissues with the
use of guanidine isothiocyanate.21 Reverse-transcription
polymerase chain reaction Southern blot analysis with specific
oligonucleotide probes for human tissue kallikrein
(5'-primer, 5'-AACACAGCCCAGTTTGT-3'; 3'-primer,
5'-CTTCACATAAGACAGCA-3'; internal probe, 5'-GACCTCAAAATCCTGCC-3') was
performed as previously described.22
ELISA for Human Tissue Kallikrein
Human tissue kallikrein levels in rat serum were determined with
the use of an ELISA specific for human tissue
kallikrein.23 Human tissue kallikrein standard ranges from
0.4 to 25 ng/mL. Because the antibody recognizes only the active
kallikrein, the immunoreactive kallikrein levels determined with ELISA
represent active kallikrein.
Radioimmunoassays for Kinin, cGMP, and cAMP
The heart was homogenized in 10 vol of 0.1 N HCl at
4°C. The homogenates were centrifuged at
15 000g for 30 minutes, and aliquots of the supernatants
were used for the assay. Protein concentrations were measured according
to the method of Lowry et al.24 Cardiac kinin, cGMP,
and cAMP levels were measured with the use of
radioimmunoassays.25 26 27 28
Statistical Analysis
Data are expressed as mean±SEM and were compared between
experimental groups with the use of 1-way ANOVA and Fisher’s PLSD. HR
and MAP were compared among groups with the use of repeated measures
ANOVA. Binomially distributed data (VT and VF incidence) were compared
with the use of the 
2 test and Fisher’s exact
probability test. Differences were considered statistically significant
at a value of P<0.05.
|
Results |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
Hemodynamic Parameters After Gene Delivery
Table 1 summarizes the hemodynamic variables throughout the experiment. The results show that at the basal level, these parameters are comparable in all groups. Two-way repeated measures ANOVA indicated no significant changes in HR but did indicate a significant time-related reduction in MAP during the experiment. No group-related effects were detected for either MAP or HR. These results suggest that the effects of Ad.CMV-cHK, Ad.CMV-LacZ, icatibant, and a combination of Ad.CMV-cHK and icatibant did not alter the time course of these hemodynamic variables in this model under the experimental conditions.
|
Effects of Kallikrein Gene Delivery on Myocardial Infarct
Size
Figure 1 shows the effect of
kallikrein gene delivery on infarct size after coronary artery
occlusion/reperfusion. The ratio of infarct size to area at risk was
significantly lower in rats receiving Ad.CMV-cHK than in control rats
receiving either saline or Ad.CMV-LacZ (44.5±2.0% versus 72.3±3.5%
or 69.2±2.7%, n=10 or 8, P<0.01, respectively; Figure 1A). Icatibant alone did not alter the infarct size after
myocardial ischemia/reperfusion compared with control rats
injected with either saline or Ad.CMV-LacZ groups (68.2±2.4% versus
72.3±3.5% or 69.2±2.7%). However, icatibant abolished the
beneficial effect of kallikrein gene delivery on myocardial
ischemia/reperfusion (72.8±2.4% versus 44.5±2.0%, n=8 and
10, P<0.01; Figure 1A). The ratio of the area at
risk to the left ventricle was similar among the experimental and
control groups, indicating that the ischemic area was created
at the same size in all groups (Figure 1B).
|
Effects of Kallikrein Gene Delivery on Left Ventricular
Arrhythmias
Figure 2 shows the effect of
kallikrein gene delivery on the incidence of ventricular
arrhythmias induced by myocardial ischemia/reperfusion.
Ventricular premature beats were observed in all rats after
coronary artery occlusion. Kallikrein gene delivery
significantly attenuated the incidence of VF from 64.3% to 16.7%
(n=14 and 24, P<0.01, Figure 2A) and reduced the
incidence of VT from 85.7% to 41.7% (n=14 and 24, P<0.01,
Figure 2B) compared with the group injected with control
adenovirus. Neither Ad.CMV-LacZ nor icatibant affected these incidence
rates. However, icatibant reversed the protective effect of kallikrein
gene delivery on ventricular arrhythmias (Figure 2, A and B).
|
Expression of Human Tissue Kallikrein After Gene Delivery
At 7 days after the intravenous injection of
adenovirus containing the human tissue kallikrein gene into the jugular
vein of rats, human tissue kallikrein mRNA was identified through
reverse-transcription polymerase chain reaction Southern blot
analysis. Human tissue kallikrein mRNA was detected in the
heart, liver, lung, kidney, and adrenal gland (Figure 3, top left). The expression of human
tissue kallikrein mRNA was not detected in control rats receiving
Ad.CMV-LacZ (Figure 3, top right). Similar levels of ß-actin
mRNA were detected in tissue of both experimental and control groups,
indicating the integrity of RNA in these samples (Figure 3, bottom). With the use of ELISA in a time-dependent manner, recombinant
human tissue kallikrein was also detected in rat serum from 1 to 7 days
after kallikrein gene delivery, with the highest level of 274.7±10.9
ng/mL (n=3) occurring at 3 days after gene transfer. The results are
consistent with our previous studies showing the time course of
human tissue kallikrein levels after gene delivery.17 18
Because of the high efficiency of adenovirus infection, the highest
level of human tissue kallikrein in rat serum was found at 3 to 4 days
after gene transfer and was detectable within 5 to 6 weeks.
|
Kallikrein Gene Delivery Increased Cardiac Kinin and cGMP
Levels
Figure 4 shows cardiac kinin and
cGMP levels after myocardial ischemia/reperfusion injury in
rats receiving gene transfer. Adenovirus-mediated kallikrein gene
delivery significantly increased cardiac kinin levels compared with
control rats receiving Ad.CMV-LacZ (29.6±12.7 versus 6.1±2.1 pg/mg
protein, n=5, P<0.05), whereas icatibant and Ad.CMV-cHK
administration did not change kinin levels (31.9±7.5 pg/mg protein,
n=5). Cardiac cGMP levels also increased in the kallikrein group
compared with the control group (1.30±0.06 versus 0.86±0.09 pmol/mg
protein, n=7, P<0.01), and icatibant treatment
significantly reduced cGMP levels to those of control rats receiving
Ad.CMV-LacZ. No significance difference was detected in cardiac cAMP
levels between experimental and control rats (0.56±0.06 and 0.47±0.02
nmol/mg protein, respectively; n=5).
|
Kallikrein Gene Delivery Attenuated Apoptotic
Myocardiocytes
Figure 5 shows
representative apoptotic figures in
myocardiocytes after reperfusion injury. TUNEL-positive
staining of cardiomyocytes was significantly reduced in the
group of rats receiving Ad.CMV-cHK compared with the group receiving
Ad.CMV-LacZ, whereas icatibant abolished the protective effect of
kallikrein gene delivery on programmed cell death in
myocardiocytes. Figure 5B shows that kallikrein gene
delivery significantly reduced the ratio of TUNEL-positive
cardiomycytes to total cardiomyocytes compared with the
control group (24.8±3.0% versus 40.8±2.6%, n=6,
P<0.01). Icatibant abolished the beneficial effect of
kallikrein on programmed cell death in myocardiocytes
(34.6±3.6% versus 24.8±3.0%, n=6, P<0.05).
|
|
Discussion |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
This study demonstrates that adenovirus-mediated kallikrein gene delivery protects cardiac function as demonstrated by the reduction in myocardial infarct size and the incidence of ventricular arrhythmias and apoptotic cardiomyocytes after acute coronary artery occlusion/reperfusion in vivo. These cardioprotective effects of kallikrein were blocked by icatibant, a specific bradykinin B2 receptor antagonist, indicating a kinin-mediated event. Human kallikrein mRNA was detected in rat heart and immunoreactive human kallikrein was secreted into the circulation after the kallikrein gene transfer. Kallikrein gene delivery resulted in increased cardiac kinin and cGMP levels. These results indicate that the protective effects of kallikrein gene delivery in myocardial ischemia/reperfusion injury are mediated via kinin through an NO-cGMP–dependent signal transduction pathway.
Ischemia/reperfusion injury is a multifactorial event that includes calcium overload, free radical production, metabolic abnormalities with associated acidosis, and inflammatory reactions. Many studies have demonstrated myocardial protection from ischemia/reperfusion injury. Although reports on this topic are controversial, it is generally accepted that myocardial protection during reperfusion is beneficial and necessary. A protective role of bradykinin B2 receptor in this regard has been implicated,7 8 but the mechanisms by which kinin-mediated protection is provided in ischemic damage are not clear. There are several possibilities regarding the cardioprotective effects of kinin. First, locally and systemically administered kinins can increase coronary and capillary nutritional flow.7 29 Second, kinins may change cardiac metabolism, such as the preservation of high-energy–enriched phosphates and increase in myocardial glucose uptake and use7 30 ; these 2 combined events improve cardiac function and reduce the risk of necrosis. Third, infusions of kinins reduce ischemia-induced norepinephrine overflow31 and reperfusion-induced arrhythmias.7 32 The protection may lead to decreases in cytosolic enzyme leakage, as well as in superoxides that induce cell damage. This in turn may break a vicious circle of cell injury and death. Therefore, these combined effects of kinins on the myocardium may provide protection against ischemia.
The inhibition of ACE has been shown to reduce infarct size and improve cardiac function after coronary artery occlusion/reperfusion injury.4 5 6 ACE inhibitors not only inhibit angiotensin II production but also augment kinin accumulation by decreasing kinin degradation. The finding that a specific bradykinin B2 receptor antagonist could reverse the cardioprotective effects of ACE inhibition in myocardial ischemia was thought to be mediated, at least in part, via activation at the B2 receptor.8 In this study, we observed that kallikrein gene delivery reduced not only the infarct size but also the incidence of ventricular arrhythmias (Figure 2). It is apparent that minimization of the infarct size leads to a reduced incidence of arrhythmias. Previous studies showed that low doses of bradykinin reduced ischemic arrhythmias and improved myocardial electrical stability while not having an effect on coronary blood flow.7 32 33 Bradykinin reduced the severity of ventricular arrhythmias induced by short-term occlusion/reperfusion, even before necrosis occurred.7 32 These findings indicate that the activation of bradykinin receptors may cause an alteration in myocardial electrophysiological activities against ventricular arrhythmias. Taken together, the results of this study indicate that increased cardiac kinin might directly reduce the incidence of VT and VF.
The cardioprotective effects of kinins could be mediated by the activation of bradykinin B2 receptor via the pathways through either phospholipase A2 or phospholipase C. The stimulation of phospholipase A2 results in increased prostacyclin formation and its metabolites, such as 6-keto-prostaglandin F1 and prostaglandin F2, were implicated as a consequence of elevated kallikrein activity and local kinin formation.34 The binding of prostacyclin to its receptor may result in the stimulation of adenylate cyclase and increased cAMP levels. Our results show that cardiac cAMP levels were not changed after kallikrein gene delivery compared with the control, indicating that cAMP may not serve as a second messenger in kinin-mediated cardioprotective effects. Alternatively, activation of bradykinin B2 receptor may stimulate phospholipase C and thus trigger NO formation. Increased NO formation may result in the stimulation of guanylate cyclase and increased cGMP levels.35 It has been shown in cultured porcine aortic endothelial cells that bradykinin stimulates the release of NO, which in turn induces the production of cGMP via activation of bradykinin B2 receptors.36 In this study, we detected increased cardiac cGMP levels in the group receiving kallikrein gene therapy (Figure 4). These results indicate that the NO-cGMP signal transduction pathway is more likely to serve as the second messenger after the binding of intact kinin to the bradykinin B2 receptor in this model.
In the present study, we show that kallikrein gene delivery
significantly reduced myocardiocyte apoptosis after
ischemia/reperfusion injury as demonstrated with the TUNEL
assay and that icatibant administration reversed the
kallikrein-mediated beneficial effect. Myocardial
ischemia/reperfusion induces myocardial infarction and
apoptosis. Most cardiomyocytes that yield to
myocardial infarction during the most damaging phase of the insult
undergo apoptosis within 6 hours, whereas necrosis becomes the
major contributor to cell death 6 hours after the onset of the
ischemic insult.37 Therefore, we mainly identified
apoptosis in cardiomyocytes after a 30-minute
coronary occlusion followed by a 2-hour reperfusion. Kinin and
cGMP levels in rat heart were significantly increased after kallikrein
gene delivery in this model, and icatibant treatment reduced cGMP
levels to those of control rats. These findings suggest that the
binding of cardiac kinins to bradykinin B2
receptors activates second messengers such as NO and cGMP and
thus leads to inhibition of apoptosis in the
ischemia/reperfusion injury model. This notion is
consistent with previous studies showing that exogenous NO
could inhibit caspase-3–like activity and prevent tumor necrosis
factor-
–induced apoptosis in endothelial
cells via a cGMP–dependent pathway.38 39 Furthermore,
inhibition of caspase activity by its specific inhibitor
has been shown to attenuate both apoptosis and
ischemia/reperfusion injury in rat
myocardium.11 Apoptosis that
contributes to the extension of the infarct is one of the major events
responsible for ventricular remodeling. Therefore, the
potential benefits of antiapoptotic effects after kallikrein
gene delivery may ascribe to a reduction in myocardial infarct size and
thus the development of congestive heart failure. Taken together, the
present results indicate that the cardiac kallikrein-kinin system
plays a protective role in the protection against apoptosis and
myocardial infarction.
In clinical situations, ischemia/reperfusion–induced myocardial damage commonly occurs during thrombolytic therapy for myocardial infarction, restoration of blood flow after cardioplegic arrest in cardiovascular surgery, and heart transplantation. The present study showed that the delivery of the kallikrein gene was effective in reducing myocardial ischemia/reperfusion injury in rats. The successful application of kallikrein gene therapy may have potential value in the treatment of individuals with coronary artery diseases.
|
Acknowledgments |
|---|
This work was supported by National Institutes of Health grants HL-29397 and HL-52196. We thank Dr Jo Anne Simson (Professor Emeritus in the Department of Cell Biology and Anatomy, Medical University of South Carolina) for critical evaluation of programmed cell death with the TUNEL assay in the ischemic myocardium.
Received June 18, 1999; first decision July 20, 1999; accepted August 27, 1999.
|
References |
|---|
|
TopAbstractIntroductionMethodsResultsDiscussionReferences |
|---|
- Braunwald E, Kloner RA. Myocardial reperfusion: a double-edged sword? J Clin Invest. 1985;76:1713–1719.
- Garg R, Yusuf S. Overview of randomized trials of angiotensin-converting enzyme inhibitors on mortality and morbidity in patients with heart failure. JAMA. 1995;273:1450–1456.
- Schmieder RE, Martus P, Klingbel A. Reversal of left ventricular hypertrophy in essential hypertension: a meta-analysis of randomized double-blind studies. JAMA. 1996;275:1507–1513.[Abstract]
-
Ertl G, Kloner RA, Alexander RW, Braunwald E.
Limitation of experimental infarct size by
angiotensin-converting enzyme inhibitor.
Circulation. 1982;65:40–48.
[Free Full Text] - Carl E, Hoch C, Riberio LGT, Lefer AM. Preservation of ischemic myocardium by a new converting enzyme inhibitor, enalaprilic acid, in acute myocardial infarction. Am Heart J. 1986;109:222–228.
- Linz W, Scholkens BA, Han YF. Beneficial effects of the converting enzyme inhibitor, ramipril, in ischemic rat hearts. J Cardiovasc Pharmacol. 1986;8(suppl 10):S91–S99.
- Linz W, Wiemer G, Scholkens BA. Role of kinins in the pathophysiology of myocardial ischemia: in vitro and in vivo studies. Diabetes. 1996;45(suppl 1):551–558.
- Martorana PA, Kettenbach B, Breipohl G, Linz W, Scholkens BA. Reduction of infarct size by local angiotensin-converting enzyme inhibition is abolished by a bradykinin antagonist. Eur J Pharmacol. 1990;182:395–396.[Medline] [Order article via Infotrieve]
-
Liu Y-H, Yang X-P, Sharov VG, Sigmon DH, Sabbah HN,
Carretero OA. Paracrine systems in the cardioprotective effect of
angiotensin-converting enzyme inhibitors on
myocardial ischemia/reperfusion injury in rats.
Hypertension. 1996;27:7–13.
[Abstract/Free Full Text] -
Fliss H, Gattinger D. Apoptosis in
ischemic and reperfused rat myocardium. Circ
Res. 1996;79:949–956.
[Abstract/Free Full Text] -
Yaoita H, Ogawa K, Maehara K, Maruyama Y. Attenuation
of ischemia/reperfusion injury in rats by a caspase
inhibitor. Circulation. 1998;97:276–281.
[Abstract/Free Full Text] - Xiong W, Chen LM, Woodley-Miller C, Simson JA, Chao J. Identification, purification, and localization of tissue kallikrein in rat heart. Biochem J. 1990;267:639–646.[Medline] [Order article via Infotrieve]
-
Nolly H, Carbini LA, Scicli G, Carretero OA, Scicli AG.
A local kallikrein-kinin system is present in rat heart.
Hypertension. 1994;23:919–923.
[Abstract/Free Full Text] - Hess JF, Borkowski JA, Young GS, Strader CD, Ransom RW. Cloning and pharmacological characterization of a human bradykinin (BK-2) receptor. Biochem Biophys Res Commun. 1992;184:260–268.[Medline] [Order article via Infotrieve]
- Clements JA. The glandular kallikrein family of enzymes: tissue-specific expression and hormonal regulation. Endocr Rev. 1989;10:393–419.[Medline] [Order article via Infotrieve]
- Bhoola KD, Figueroa CD, Worthy K. Bioregulation of kinins: kallikreins, kininogens, and kininases. Pharmacol Rev. 1992;44:1–8.[Medline] [Order article via Infotrieve]
- Chao J, Zhang JJ, Lin KF, Chao L. Human kallikrein gene delivery attenuates hypertension, cardiac hypertrophy and renal injury in Dahl salt-sensitive rats. Hum Gene Ther. 1998;9:21–31.[Medline] [Order article via Infotrieve]
-
Yayama K, Wang C, Chao L, Chao J. Kallikrein gene
delivery attenuates hypertension and cardiac hypertrophy
and enhances renal function in Goldblatt hypertensive rats.
Hypertension. 1998;31:1104–1110.
[Abstract/Free Full Text] - Walker MJA, Curtis MJ, Hearse DJ, Campbell RWF, Janse MJ, Yellon DM, Cobbe SM, Coker SJ, Harness JB, Harron DWG, Higgins AJ, Julian DG, Lab MJ, Manning AS, Northover BJ, Parratt JR, Riemersma RA, Riva E, Russell DC, Sheridan DJ, Winslow E, Woodward B. The Lambeth Conventions: guidelines for the study of arrhythmias in ischemia, infarction, and reperfusion. Cardiovasc Res. 1988;22:447–455.[Medline] [Order article via Infotrieve]
- Negoescu A, Guillermet C, Lorimier P, Robert C, Lantuejoul S, Brambilla E, Labat-Moleur C. TUNEL apoptotic cell detection in archived paraffin-embedded tissues. Biochemica. 1998;3:36–41.
- Davis LG, Dibner MD, Battey JF. Basic Methods in Molecular Biology. New York, NY: Elsevier Science Publishing Co Inc; 1986:130–135.
-
Xiong W, Chao L, Chao J. Muscle delivery of human
tissue kallikrein gene reduces blood pressure in spontaneously
hypertensive rats. Hypertension. 1995;25:715–719.
[Abstract/Free Full Text] - Wang C, Chao L, Chao J. Direct gene delivery of human tissue kallikrein reduces blood pressure in spontaneously hypertensive rats. J Clin Invest. 1995;95:1710–1716.
-
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein
measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265–275.
[Free Full Text] - Brooker G, Harper JF, Terasaki WL, Moylan RD. Radioimmunoassay of cyclic AMP and cyclic GMP. Adv Cycl Nucl Res. 1979;10:1–33.[Medline] [Order article via Infotrieve]
- Gettys TW, Okonogi K, Tarry WC, Johnston J, Horton C, Taylor IL. Examination of relative rates of cAMP synthesis and degradation in crude membranes of adipocytes treated with hormones. Second Messengers Phosphorprot. 1990;13:37–50.
- Harper JF, Brooker G. Femtomole sensitive radioimmunoassay for cyclic AMP and cyclic GMP after 2'O acetylation by acetic anhydride in aqueous solution. J Cyclic Nucleotide Res. 1975;1:207–218.[Medline] [Order article via Infotrieve]
- Shimamoto K, Ando T, Nakao T, Tanaka S, Sakuma M, Miyahara M. A sensitive radioimmunoassay method for urinary kinin in man. J Lab Clin Med. 1978;91:721–728.[Medline] [Order article via Infotrieve]
- Lochner W, Parratt JR. A comparison of the effects of locally and systemically administered kinins on coronary blood flow and myocardial metabolism. Br J Pharmacol. 1966;26:17–26.[Medline] [Order article via Infotrieve]
- Scholkens BA, Linz W. Bradykinin-mediated metabolic effects in isolated perfused rat hearts. Agents Actions. 1992;38(suppl II):36–42.
- Carlsson L, Abrahamsson T. Ramiprilat attenuates local ischemia-induced release of noradrenaline in the ischemic myocardium. Eur J Pharmacol. 1989;166:157–164.[Medline] [Order article via Infotrieve]
- Vegh A, Szekeres L, Parratt JR. Local intracoronary infusions of bradykinin profoundly reduce the severity of ischemia-induced arrhythmias in anaesthetized dogs. Br J Pharmacol. 1991;104:294–295.[Medline] [Order article via Infotrieve]
- Tobe TJM, de Langen CDJ, Tio RA, Bel KJ, Mook PH, Wesseling H. In vivo effect of bradykinin during ischemia and reperfusion: improved electrical stability two weeks after myocardial infarction in pig. J Cardiovasc Pharmacol. 1991;17:600–607.[Medline] [Order article via Infotrieve]
- Mullane KM, Moncada S. Prostacyclin release and the modulation of some vasoactive hormones. Prostaglandins. 1980;20:25–49.[Medline] [Order article via Infotrieve]
- Linz W, Wiemer G, Gohlke P, Unger T, Scholkens BA. Contribution of kinins to the cardiovascular actions of angiotensin-converting enzyme inhibitors. Pharmacol Rev. 1995;47:25–49.[Abstract]
-
Schini VB, Boulanger C, Regoli D, Vanhoutte PM.
Bradykinin stimulates the production of cGMP via activation of
B2 kinin receptors in cultured porcine aortic
endothelial cells. J Pharmacol Exp
Ther. 1990;252:581–585.
[Abstract/Free Full Text] - Kajstura J, Cheng W, Reiss K, Clark WA, Sonnenblick EH, Krajewski S, Reed JC, Olivetti G, Anversa P. Apoptotic and necrotic myocyte cell deaths are independent contributing variables of infarct size in rats. Lab Invest. 1996;74:86–107.[Medline] [Order article via Infotrieve]
-
Kim YM, Talanian RV, Billiar TR. Nitric oxide inhibits
apoptosis by preventing increases in caspase-3-like activity
via two distinct mechanisms. J Biol Chem. 1997;272:31138–31148.
[Abstract/Free Full Text] - Shen YH, Wang XL, Wilcken DE. Nitric oxide induces and inhibits apoptosis through different pathways. FEBS Lett. 1998;433:125–131.[Medline] [Order article via Infotrieve]
This article has been cited by other articles:
 |
|
 |
|
  Y.-Y. Yao, H. Yin, B. Shen, R. S. Smith Jr, Y. Liu, L. Gao, L. Chao, and J. Chao Tissue kallikrein promotes neovascularization and improves cardiac function by the Akt-glycogen synthase kinase-3{beta} pathway Cardiovasc Res, September 4, 2008; (2008) cvn223v2. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Yuan, J. Deng, T. Wang, C. Zhao, X. Xu, P. Wang, J. W. Voltz, M. L. Edin, X. Xiao, L. Chao, et al. Tissue Kallikrein Reverses Insulin Resistance and Attenuates Nephropathy in Diabetic Rats by Activation of Phosphatidylinositol 3-Kinase/Protein Kinase B and Adenosine 5'-Monophosphate-Activated Protein Kinase Signaling Pathways Endocrinology, May 1, 2007; 148(5): 2016 - 2026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chao, H.-J. Li, Y.-Y. Yao, B. Shen, L. Gao, G. Bledsoe, and L. Chao Kinin Infusion Prevents Renal Inflammation, Apoptosis, and Fibrosis via Inhibition of Oxidative Stress and Mitogen-Activated Protein Kinase Activity Hypertension, March 1, 2007; 49(3): 490 - 497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Fiedler, R. Feil, F. Hofmann, C. Willenbockel, H. Drexler, A. Smolenski, S. M. Lohmann, and K. C. Wollert cGMP-dependent Protein Kinase Type I Inhibits TAB1-p38 Mitogen-activated Protein Kinase Apoptosis Signaling in Cardiac Myocytes J. Biol. Chem., October 27, 2006; 281(43): 32831 - 32840. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Bak, I. Lekli, B. Juhasz, N. Nagy, E. Varga, J. Varadi, R. Gesztelyi, G. Szabo, L. Szendrei, I. Bacskay, et al. Cardioprotective mechanisms of Prunus cerasus (sour cherry) seed extract against ischemia-reperfusion-induced damage in isolated rat hearts Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1329 - H1336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-F. Xia, R. S. Smith Jr, B. Shen, Z.-R. Yang, C. V. Borlongan, L. Chao, and J. Chao Postischemic Brain Injury Is Exacerbated in Mice Lacking the Kinin B2 Receptor Hypertension, April 1, 2006; 47(4): 752 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Montanari, H. Yin, E. Dobrzynski, J. Agata, H. Yoshida, J. Chao, and L. Chao Kallikrein Gene Delivery Improves Serum Glucose and Lipid Profiles and Cardiac Function in Streptozotocin-Induced Diabetic Rats Diabetes, May 1, 2005; 54(5): 1573 - 1580. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chao and L. Chao Kallikrein-kinin in stroke, cardiovascular and renal disease Exp Physiol, May 1, 2005; 90(3): 291 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yin, L. Chao, and J. Chao Kallikrein/Kinin Protects against Myocardial Apoptosis after Ischemia/Reperfusion via Akt-Glycogen Synthase Kinase-3 and Akt-Bad{middle dot}14-3-3 Signaling Pathways J. Biol. Chem., March 4, 2005; 280(9): 8022 - 8030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-F. Xia, H. Yin, C. V. Borlongan, L. Chao, and J. Chao Kallikrein Gene Transfer Protects Against Ischemic Stroke by Promoting Glial Cell Migration and Inhibiting Apoptosis Hypertension, February 1, 2004; 43(2): 452 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Sharma Does the Kinin System Mediate in Cardiovascular Abnormalities? An Overview J. Clin. Pharmacol., November 1, 2003; 43(11): 1187 - 1195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bledsoe, L. Chao, and J. Chao Kallikrein gene delivery attenuates cardiac remodeling and promotes neovascularization in spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1479 - H1488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kato, H. Yin, J. Agata, H. Yoshida, L. Chao, and J. Chao Adrenomedullin gene delivery attenuates myocardial infarction and apoptosis after ischemia and reperfusion Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1506 - H1514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P A J Krijnen, R Nijmeijer, C J L M Meijer, C A Visser, C E Hack, and H W M Niessen Apoptosis in myocardial ischaemia and infarction J. Clin. Pathol., November 1, 2002; 55(11): 801 - 811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Agata, L. Chao, and J. Chao Kallikrein Gene Delivery Improves Cardiac Reserve and Attenuates Remodeling After Myocardial Infarction Hypertension, November 1, 2002; 40(5): 653 - 659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Emanueli, M. B. Salis, A. Pinna, T. Stacca, A. F. Milia, A. Spano, J. Chao, L. Chao, L. Sciola, and P. Madeddu Prevention of Diabetes-Induced Microangiopathy by Human Tissue Kallikrein Gene Transfer Circulation, August 20, 2002; 106(8): 993 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Porcu, C. Emanueli, M. Kapatsoris, J. Chao, L. Chao, and P. Madeddu Reversal of Angiogenic Growth Factor Upregulation by Revascularization of Lower Limb Ischemia Circulation, January 1, 2002; 105(1): 67 - 72. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. FRANCIS, M. K. RAIZADA, A. A. MANGI, L. G. MELO, V. J. DZAU, P. R. VALE, J. M. ISNER, D. W. LOSORDO, J. CHAO, M. J. KATOVICH, et al. Genetic targeting for cardiovascular therapeutics: are we near the summit or just beginning the climb? Physiol Genomics, December 21, 2001; 7(2): 79 - 94. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||