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(Hypertension. 2001;38:367.)
© 2001 American Heart Association, Inc.

Scientific Contributions

Angiotensin II Type 2 Receptor Inhibits Epidermal Growth Factor Receptor Transactivation by Increasing Association of SHP-1 Tyrosine Phosphatase

Yasunobu Shibasaki; Hiroaki Matsubara; Yoshihisa Nozawa; Yasukiyo Mori; Hiroya Masaki; Atsushi Kosaki; Yoshiaki Tsutsumi; Yoko Uchiyama; Soichiro Fujiyama; Atsuko Nose; Osamu Iba; Eriko Tateishi; Takamasa Hasegawa; Masatsugu Horiuchi; Clara Nahmias; Toshiji Iwasaka

From the Department of Medicine II, Kansai Medical University (Y.S., H. Matsubara, Y.M., H. Masaki, A.K., Y.T., Y.U., S.F., A.N., O I., E.T., T.H., T.I.), Moriguchi, Osaka, Japan; Pharmacological Laboratory, Taiho Pharmaceutical Co Ltd (Y.N.), Tokushima, Japan; Department of Medical Biochemistry, Ehime University School of Medicine (M.H.), Ehime, Japan; and Institut Cochin de Genetique Moleculaire, Centre Nattionale de Recherche Scientifique (C.N.), Paris, France.

Correspondence to Hiroaki Matsubara, MD, PhD, Department of Medicine II, Kansai Medical University, Fumizonocho 10-15, Moriguchi, Osaka 570-8507, Japan. E-mail matsubah{at}takii.kmu.ac.jp

	Abstract

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Abstract—— Angiotensin (Ang) II has 2 major receptor isoforms, Ang type 1 (AT₁) and Ang type (AT₂). AT₁ transphosphorylates epidermal growth factor receptor (EGFR) to activate extracellular signal–regulated kinase (ERK). Although AT₂ was shown to inactivate ERK, the action of AT₂ on EGFR activation remains undefined. Using AT₂-overexpressing vascular smooth muscle cells from AT₂ transgenic mice, we studied these undefined actions of AT₂. Maximal ERK activity induced by Ang II was increased 1.9- and 2.2-fold by AT₂ inhibition, which was abolished by orthovanadate but not okadaic acid or pertussis toxin. AT₂ inhibited AT₁-mediated EGFR tyrosine phosphorylation by 63%. The activity of SHP-1 tyrosine phosphatase was significantly upregulated 1 minute after AT₂ stimulation and association of SHP-1 with EGFR was increased, whereas AT₂ failed to tyrosine phosphorylate SHP-1. Stable overexpression of SHP-1–dominant negative mutant completely abolished AT₂-mediated inhibition of EGFR and ERK activation. AT₁-mediated c-fos mRNA accumulation was attenuated by 48% by AT₂ stimulation. Induction of fibronectin gene containing an AP-1 responsive element in its 5'-flanking region was decreased by 37% after AT₂ stimulation, corresponding to the results of gel mobility assay with the AP-1 sequence of fibronectin as a probe. These findings suggested that AT₂ inhibits ERK activity by inducing SHP-1 activity, leading to decreases in AP-1 activity and AP-1–regulated gene expression, in which EGFR dephosphorylation plays an important role via association of SHP-1.

Key Words: angiotensin II • angiotensin II receptors • angiotensin II type 2 receptor • tyrosinephosphatase • SHP-1 • epidermal growth factor receptor

	Introduction

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Angiotensin (Ang) II plays a critical role in regulation of the cardiovascular system via 2 main Ang II receptor subtypes, Ang type 1(AT₁) and Ang type 2(AT₂).^1,2 Most of the Ang II–mediated vasoconstrictive actions are mediated by AT₁, although little information is available regarding signal transduction by AT₂.³ AT₂ is abundantly and widely expressed in fetal tissues and is reexpressed in myocardial infarction and vascular injury.⁴ Although the level of expression of AT₂ is low in large vessels, AT₂ is present in high levels in vascular smooth muscle cells (VSMCs) of microvessels.⁵ AT₂ antagonizes the in vivo effects of AT₁ on blood pressure⁶ and renal natriuresis⁷ and mediates growth inhibition,^8,9 differentiation,¹⁰ and/or apoptosis¹¹ in VSMCs and in endothelial, neuronal, and fibroblast R3T3 cells.

These effects of AT₂ are mediated mainly by the activation of protein tyrosine phosphatases (PTP), resulting in the inactivation of AT₁-activated extracellular signal–regulated kinase (ERK).^12–14 Recently, Ang II–induced ERK activation was shown to be mediated by epidermal growth factor receptor (EGFR) transactivated via AT₁ in a Ca²⁺ signal–dependent manner in cardiac fibroblasts,¹⁵ VSMCs,¹⁶ and liver epithelial cells.¹⁷ In contrast, activation of c-Jun NH₂-terminal kinase (JNK) by AT₁ is dependent on Ca²⁺-sensitive tyrosine kinase Pyk2 activity.^18,19 AT₂ was shown to inactivate ERK via mitogen-activated protein (MAP) kinase phosphatase-1 (MKP-1),¹¹ src homology 2 domain–containing PTP (SHP-1),^13,20 or serine-threonine phosphatase PP2A¹⁴ in NIE-115 and PC12W cells. Although AT₂ has also been shown to inhibit tyrosine phosphorylation of signal transducers and activators of transcription²¹ and insulin receptor,²² it remained unclear whether AT₂ signaling dephosphorylates EGFR to affect ERK activity.

We established AT₂-overexpressing VSMCs (AT₂-VSMCs) from VSMC-targeted AT₂ transgenic (TG) mice⁶ and attempted to define the AT₂ signaling in VSMCs of microvessels endogenously expressing AT₂. In the present study, we showed that (1) AT₂ dephosphorylates EGFR by inducing SHP-1 activation and its association with EGFR, leading to ERK inactivation, and (2) inhibition of ERK activity causes a decrease in c-fos expression and AP-1 activity, leading to a decrease in the expression of AP-1–responsive genes such as fibronectin.

	Methods

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Materials
Antibodies were purchased from the following sources: Santa Cruz (c-fos, EGFR), Transduction Laboratories (SHP-1), New England BioLabs (phospho-specific ERK), and Upstate Biotechnology (4G10-HRP, ERK). AT₁ antagonist RNH-6270 (active form of olmesartan) was provided by Sankyo Pharmaceutical Co Ltd (Tokyo, Japan).

Cell Culture
AT₂-VSMCs isolated from the aorta of AT₂ TG mice were reported to overexpress AT₂ without affecting AT₁ numbers, and no AT₂ was detectable in wild VSMCs.⁶ We used AT₂-VSMCs at passage 4 (AT₂/AT₁ ratio, 48%; AT₁, 46±3 fmol/mg protein). Subconfluent cells were starved of serum for 24 hours and used for the experiments.

Assays for ERK and SHP-1 PTP Activities
ERK activities were determined by the immune-complex kinase assay with the use of anti-ERK antibodies as described previously;^15,19 immunoprecipitates were reacted with synthetic peptide (KRELVEPLTPAGEAPNQALLR) for ERK as a substrate. PTP activity in SHP-1 immunoprecipitates was determined by use of abl-tyrosine–phosphorylated myelin basic protein (MBP) as a substrate as described previously.^13,20 The immunoblotting signals were visualized by chemiluminescence and quantified by densitometry.

Transfection of DNA, Northern Blotting, and Calcium Analysis
Because the efficiency for DNA transfection was low in VSMCs, we established stably transfected cell lines as reported previously.^15,19 Briefly, AT₂-VSMCs were cotransfected with wild-type mouse AT₂ in pBC-SF (10 µg) and SHP-1 (C453/S) mutant in pcDNA3 (20 µg)²⁰ using LIPOFECTAMINE PLUS (GIBCO BRL). After selected with G418, 2 stable clones that expressed the highest levels of AT₂ (determined by the binding assay) were selected (the Table). Total RNA was analyzed with c-fos or GAPDH cDNA probes.^15,19 Ca²⁺ levels were measured with fura 2-AM as reported.²³

View this table: [in this window] [in a new window]   Table 1. AT1 and AT2 Numbers in Stable Cell Lines Overexpressing SHP-1–Dominant Negative Mutant

Gel Retardation Assay
The gel retardation assay using oligonucleotides corresponding to a DNA fragment containing an AP-1 sequence of rat fibronectin gene (between nt-438 and -473) as a probe was performed as previously reported.²⁴ The core AP-1 sequence (TGACGCA at -453TGGATAA) was mutated by site-directed mutagenesis, and supershift assay was performed by adding anti–c-fos or anti–c-jun antibodies (1 µg each) to the samples, followed by incubation for 1 hour at room temperature.²³

Statistical Analysis
The results are expressed as mean±SE. Statistical analyses were performed by 1-way ANOVA followed by pairwise contrast (control versus condition) with Dunnett’s multiple comparison test. Data were considered statistically significant at P<0.05.

	Results

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AT₂ Induces ERK Inactivation and Increases PTP Activity
Addition of Ang II (100 nmol/L) to wild VSMCs stimulated ERK activity with a maximal increase (10.4-fold) at 7 minutes, whereas in AT₂-VSMCs isolated from AT₂ TG mice, the maximal increase in ERK was only 6.7-fold (Figure 1A). Ang II dose dependently activated ERK with a maximal peak at 100 nmol/L in both AT₂ and wild VSMCs (data not shown). AT₁ and AT₂ stimulation by addition of Ang II induced a moderate increase in ERK activation, which was further increased by 1.9-fold after AT₂ inhibition (Ang II+PD 123,319) (Figure 1B). ERK activation by Ang II was abolished by the specific AT₁ antagonist olmesartan. ERK levels activated by Ang II through AT₁ and AT₂ were increased by nonselective tyrosine-phosphatase vanadate but not by the serine-threonine phosphatase inhibitor okadaic acid or inhibition of G_i by pertussis toxin (PTX) (Figure 1B). These findings suggest that the mechanism for Ang II–mediated ERK activation is not sensitive to inhibition of serine-threonine phosphatases or inhibition of G_i by PTX. Because the data with vanadate should be carefully interpreted, we next examined whether PTP activities are increased after AT₂ stimulation. PTP activities were measured with tyrosine-phosphorylated MBP used as a substrate. PTP activities were significantly increased 1 minute after AT₂ stimulation and reached a maximum at 5 minutes (Figure 1C), similar to the time course of ERK activation.

View larger version (36K): [in this window] [in a new window]   Figure 1. Effects of AT2 on activity and phosphorylation of ERK and tyrosine phosphatase activity. ERK activity and phosphorylation were measured by the immune-complex kinase assay and Western blots using anti–phospho-ERK antibody, respectively. A, VSMCs were challenged with Ang II (100 nmol/L) for the indicated periods. B, To test the effects of PD 123,319 (1 µmol/L), AT1 antagonist olmesartan (1 µmol/L), orthovanadate (100 nmol/L), or okadaic acid (100 nmol/L), AT2-VSMCs were pretreated with these compounds for 1 hour and then exposed to Ang II (100 nmol/L) for 7 minutes. PTX (1 µg/mL) was preincubated for 24 hours. Exposure to Ang II+PD 123,319 and Ang II+olmesartan caused selective activation of AT1 and AT2, respectively. C, Tyrosine-phosphatase activities in AT2-VSMCs exposed to Ang II+olmesartan (AT2 stimulation) were determined with abl-tyrosine–phosphorylated MBP as a substrate. Results shown are mean±SE (n=4). *P<0.01 vs time 0 control or unstimulated cells.

AT₂ Inhibits Tyrosine Phosphorylation of EGFR
Because we reported that AT₁ transactivated EGFR to mediate EGFR-Ras-ERK signals,²⁴ we next studied the effect of AT₂ on EGFR phosphorylation. Ang II moderately stimulated tyrosine phosphorylation of EGFR compared with unstimulated levels (Figure 2A). Inhibition of AT₂ function by PD 123,319 or addition of vanadate further increased Ang II–induced EGFR phosphorylation by 63% or 58% (P<0.01 versus Ang II alone), respectively. Although EGFR activation by AT₁ was reported to be mediated mainly by intracellular Ca²⁺ level,^15,16 PD 123,319 treatment did not affect Ang II–mediated Ca²⁺ mobilization, and AT₁ inhibition by olmesartan abolished this Ca²⁺ mobilization (Figure 2B), suggesting no involvement of AT₂ signals in the intracellular Ca²⁺ level.

View larger version (28K): [in this window] [in a new window]   Figure 2. AT2-mediated effect on EGFR phosphorylation and intracellular Ca2+ levels. A, AT2-VSMCs treated with Ang II (100 nmol/L) for 2 minutes were immunoprecipitated with anti-EGFR antibody and then blotted with anti-phosphotyrosine antibody (PY). The same membranes were stripped and reprobed with anti-EGFR antibody. Data analyses were performed as described in Figure 1, and results shown are mean±SE (n=4). B, AT2-VSMCs were incubated in the presence of fura 2-AM and stimulated with Ang II (100 nmol/L). Traces are typical of those from 3 separate experiments. *P<0.05, **P<0.01 vs control.

SHP-1 Is Involved in AT₂-Mediated Tyrosine Dephosphorylation of EGFR
SHP-1 was shown to mediate ERK inactivation by AT₂ in neuronal cells.^13,20 SHP-1 activity with tyrosine-phosphorylated MBP as a substrate was significantly stimulated as rapidly as 1 minute after AT₂ stimulation (Ang II+olmesartan), reaching a maximum peak after 5 minutes (Figure 3A). AT₁ stimulation (Ang II+PD 123,319) did not induce any increase in SHP-1 activity (data not shown).

View larger version (43K): [in this window] [in a new window]   Figure 3. AT2-mediated SHP-1 activation and association of SHP-1 with EGFR. A, AT2-VSMCs were stimulated with Ang II (100 nmol/L) in the presence of olmesartan (1 µmol/L) for the indicated periods and then immunoprecipitated with anti–SHP-1 antibody. Tyrosine-phosphatase activities in the immunoprecipitates were determined with abl-tyrosine–phosphorylated MBP (n=4). B, AT2-VSMCs exposed to Ang II (100 nmol/L) for 2 or 5 minutes were immunoprecipitated with anti-EGFR or anti–SHP-1 antibodies, respectively, followed by blotting with anti–SHP-1 or anti-phosphotyrosine antibody (PY). FCS indicates 20% of fetal calf serum. C, In AT2-VSMCs stably overexpressing SHP-1 C453/S mutant (SHP-1–DN1 and SHP-1–DN2), EGFR and ERK phosphorylation were determined 2 and 7 minutes after Ang II treatment, respectively. ERK phosphorylation was measured as described in Figure 1. Each experiment was repeated 3 times, and representative data for SHP-1–DN1 are shown. *P<0.05, **P<0.01 vs control.

AT₂ stimulation (Ang II+olmesartan) but not AT₁ stimulation (Ang II+PD 123,319) increased the association of SHP-1 with EGFR (Figure 3B). In contrast, SHP-1 itself was not tyrosine phosphorylated by AT₂ or AT₁ stimulation, although fetal calf serum induced its tyrosine phosphorylation (FigureB).

We next tested the involvement of SHP-1 in EGFR and ERK activation using a dominant negative mutant (SHP-1–DN). Figure 3C shows that AT₂-VSMCs endogenously express SHP-1 and that stable clones (SHP-1–DN) overexpress SHP-1–DN. Furthermore, the Table indicates that the expression levels of AT₁ and AT₂ are similar between AT₂-VSMC and SHP-1–DN clones. We showed that PD 123,319 pretreatment significantly enhanced Ang II–mediated EGFR phosphorylation (Figure 2A) and ERK activation (Figure 1B), indicating the inhibitory effect of AT₂ on EGFR and ERK activity. Interestingly, in SHP-1–DN clones, EGFR and ERK activation by Ang II was not affected by PD 123,319 pretreatment (only SHP-1–DN1 data shown in Figure 3C), suggesting the involvement of SHP-1 in AT₂-mediated inactivation of EGFR and ERK.

AT₂-Mediated Inhibition of c-fos Expression
We have shown that induction of c-fos gene by Ang II is regulated mainly by EGFR-mediated signal.^15,19 We therefore tested whether AT₂ affects c-fos gene expression. Ang II moderately induced c-fos mRNA expression, whereas inhibition of AT₂ by PD 123,319 further increased Ang II–induced mRNA expression (c-fos, 81% versus Ang II alone; P<0.01) (Figure 4). AT₁ inhibition by olmesartan abolished induction of c-fos mRNA by Ang II.

View larger version (42K): [in this window] [in a new window]   Figure 4. AT2-mediated effect on c-fos expression. Total RNA (20 µg) was extracted from AT2-VSMCs exposed to Ang II (100 nmol/L) for 1 hour, and mRNA levels were analyzed by Northern blotting. Signal intensities were measured and arbitrarily normalized to GAPDH mRNA levels. Results shown are mean±SE (n=4). *P<0.01 vs control.

AT₂ Decreases Fibronectin Gene Expression by Inhibiting AP-1 Complex Formation
Induction of fibronectin transcription by Ang II was shown to be regulated by binding of AP-1 complex to the AP-1 site in the 5'-flanking region,²³ suggesting that AT₂ may downregulate fibronectin gene expression. As shown in Figure 5A, induction of fibronectin mRNA by Ang II was further increased by inhibition of AT₂ (Ang II+PD 123,319, 48%; P<0.05 versus Ang II alone) or orthovanadate treatment (51%, P<0.05 versus Ang II alone), whereas okadaic acid did not affect the mRNA levels.

View larger version (39K): [in this window] [in a new window]   Figure 5. AT2-mediated effects on fibronectin expression and AP-1 activities. A, Total RNA (20 µg) was extracted from AT2-VSMCs exposed to Ang II (100 nmol/L) for 12 hours, and mRNA levels were analyzed by Northern blotting as described in Figure 4. B, An oligonucleotide containing the AP-1 site of the fibronectin gene (nt-438 to -473) was used as a probe in the gel-shift assay. Nuclear extract was obtained from AT2-VSMCs exposed to Ang II (100 nmol/L) for 1 hour. Unlabeled oligonucleotide was used as the competitor at 25x and 100x molar excess. Mutated AP-1 oligonucleotide was used at 200x molar excess. Supershift assays were performed with anti–c-fos IgG. Densities of signals were determined and are shown relative to the control arbitrarily normalized to 1. Results shown are mean±SE (n=4). *P<0.05, **P<0.01 vs control.

We also studied the effects of AT₂ on binding of AP-1 complex to the AP-1 sequence in the fibronectin gene (Figure 5B). Ang II stimulated the binding of nuclear extract to oligonucleotides containing the AP-1 sequence of the fibronectin gene (nt-438 to -473), whereas this binding was inhibited by an excess of cold probe but not by mutation of the AP-1 core sequence. Addition of anti–c-fos antibody supershifted the binding of nuclear extract. Pretreatment with PD 123,319 significantly (P<0.05) enhanced Ang II–induced AP-1 activity by 44%, suggesting that AT₂ attenuates Ang II–induced fibronectin expression by reducing AP-1 complex formation.

	Discussion

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Ang II exerts positive or negative effects on cell growth and survival, depending on which subtype of receptor (AT₁ or AT₂) is activated. ERK activation by Ang II was mediated mainly by downstream signals of EGFR transactivated by AT₁-mediated Ca²⁺ signals in VSMCs¹⁶ or cardiac fibroblasts.¹⁵ Although recent evidence has suggested that ERK inactivation and proapoptotic action of AT₂ are mediated via SHP-1 or MKP-1 in neuronal cells such as NIE¹³ or PC12^11,20 cells, it remained unknown whether AT₂ inhibits EGFR transactivation, leading to ERK inactivation. Furthermore, AT₂-mediated association of SHP-1 with EGFR has not yet been defined. This study using VSMCs overexpressing AT₂ clearly demonstrated that (1) AT₂ dephosphorylates EGFR by inducing SHP-1 activation and its association with EGFR, leading to ERK inactivation, and (2) inhibition of ERK activity causes a decrease in AP-1 activity, leading to a decrease in the expression of AP-1–responsive genes such as fibronectin.

SHP-1 is a soluble PTP containing 2 SH2 domains that allow binding to phosphotyrosines and that participate in the negative regulation of receptor tyrosine kinase pathways.²⁵ SHP-1 physically interacts with EGFR in a cell type–specific manner, whereas it appears to have little activity toward the bound EGFR.^26,27 When SHP-1 was transiently overexpressed or exogenous autophosphorylated EGFR was added, the SHP-1 could be activated to use the bound receptor as a substrate.^26,28 In this study using VSMCs, we showed for the first time that AT₂ induced the association of SHP-1 with EGFR without affecting the phosphorylation level of SHP-1 and that overexpression of SHP-1–DN completely abolished AT₂-mediated dephosphorylation of EGFR followed by ERK activation. The observation that AT₂ stimulation inhibited EGFR phosphorylation as rapidly as 1 minute after addition was in agreement with the time course of SHP-1 activation. AT₂ receptors have been shown to activate SHP-1 in neuronal cells such as NIE-115¹³ or PC12W cells,²⁰ in which SHP-1 was shown to be involved in ERK inactivation or induction of apoptosis. However, the molecules interacting with SHP-1 were not defined in these earlier studies. Li et al²⁹ reported that platelet thrombin receptor causes SHP-1 tyrosine phosphorylation in a PTX-dependent manner and suggested the role of tyrosine kinases linked to the thrombin receptor by G_i protein. However, the present study demonstrated that AT₂ inactivated ERK via a mechanism not sensitive to inhibition of G_i by PTX, consistent with the failure of AT₂ to induce SHP-1 tyrosine phosphorylation. Recently, a structural model for SHP-1 was proposed³⁰ in which SH2 domains of SHP-1 were shown to be capable of interacting with its C terminus in a phosphotyrosine-dependent manner and thereby drive the PTPase domain in an inactive conformation. It is possible that the conformational change of SHP-1 induced by AT₂ leads to the increased association of SHP-1 with EGFR and forms the basis for activation toward the receptor as observed in our study. Thus, AT₂ may have the capacity to disrupt this intramolecular interaction.

SHP-1 is predominantly expressed in hematopoietic cells and plays a key role in hematopoiesis.³¹ Although the role of SHP-1 in VSMCs has not been defined in detail, the present study suggested a novel function of SHP-1 in AT₂-mediated ERGFR inactivation followed by a growth inhibitory action. Tang et al³² reported that SHP-2 association with Ca²⁺-sensitive tyrosine kinase Pyk2 negatively regulated AT₁-mediated Pyk2 activation in endothelial cells. Chemokines stimulate the association of SHP-1 and SHP-2 with Pyk2 in T cells and positively affect Pyk2-mediating signals.³³ AT₂ also inactivates insulin receptor by decreasing SHP-2 association with insulin receptors.²² Because SHP-1 was reported to interact with SHP-2,³⁴ further studies are required to define the relationship between SHP-1 and SHP-2 in the mechanism of AT₂ activation.

Marrero et al³⁵ reported that AT₁ stimulation in VSMC tyrosine phosphorylated Janus kinase-2 (JAK2) and that electroporation of neutralizing anti–SHP-1 antibody resulted in termination of JAK2 phosphorylation. Although we tested whether SHP-1 activity is increased after AT₁ stimulation, neither SHP-1 activity nor SHP-1 phosphorylation was induced after AT₁ stimulation, suggesting that SHP-1 activity is not modulated by AT₁ stimulation in VSMCs. Although our finding contrasts with the result of Marrero et al,³⁵ the AT₁-mediated increase in SHP-1 activity is too small to be detectable in our assay (MBP dephosphorylation), or the nonselective effect resulting from electroporation of neutralizing anti–SHP-1 antibody³⁵ might be involved in the discrepant result.

This study demonstrated that AT₁-mediated c-fos expression was decreased by AT₂ stimulation. We reported that AT₂ downregulates c-fos expression by decreasing the binding activity of signal transducers and activators of transcription to the sis-inducible element of c-fos promoter.²¹ The c-fos expression is regulated by the net interaction with different transcriptional factors.³⁶ Eguchi et al¹⁶ and we¹⁵ have shown that the inhibition of EGFR function completely abolished AT₁-mediated c-fos expression, suggesting a major role of ERK-activated serum response factor. Thus, the AT₂-mediated decrease in AP-1 activity, followed by induction of AP-1–responsive genes such as fibronectin, is likely regulated by the inactivation of ERK.

Yamada et al³⁷ reported the involvement of MKP-1 but not PP2A in AT₂-mediated ERK inactivation in PC12W cells, whereas Huang et al¹⁴ showed the participation of PP2A in neurons, and Bedecs et al¹³ reported no involvement of MKP-1 or PP2A. Although we could not define the involvement of MKP-1 in AT₂-mediated effects because of low transfection efficiency of antisense oligonucleotide for MKP-1 into VSMCs, okadaic acid was found to have no significant effect on AT₂ signals. These differences might be due to a variation of cell types or might reflect the complexity of the network involved in negative regulation of ERK activity. Thus, further dissection of the AT₂ signaling pathway and identification of the cross-point with EGFR cascades may provide new perspectives for pharmacological targeting of proliferative diseases and a unique example of negative cross-talk in growth signals.

	Acknowledgments

 
This study was supported in part by research grants from the Ministry of Education, Science and Culture, Japan; Study Group of Molecular Cardiology, Japan Medical Association; Japan Smoking Foundation; and Japan Heart Foundation.

Received November 10, 2000; first decision December 5, 2000; accepted February 22, 2001.

	References

Top Abstract Introduction Methods Results Discussion References

 

1. Baker KM, Booz GW, Dostal DE. Cardiac actions of angiotensin II: role of an intracardiac renin-angiotensin system. Annu Rev Physiol. . 1992; 54: 227–241.[Medline] [Order article via Infotrieve]
2. Inagami T, Kitami Y. Angiotensin II receptor: molecular cloning, functions and regulation. Hypertens Res. . 1994; 17: 87–97.
3. Matsubara H, Inada M. Molecular insights into angiotensin II type 1 and type 2 receptors: expression, signaling and physiological function and clinical application of its antagonists. Endocr J. . 1998; 45: 137–150.[Medline] [Order article via Infotrieve]
4. Matsubara H. Pathophysiological roles of angiotensin II type 2 receptor in cardiovascular and renal diseases. Circ Res. . 1998; 83: 1182–1191.[Abstract/Free Full Text]
5. Nora EH, Munzenmaier DH, Hansen-Smith FM, Lombard JH, Greene AS. Localization of the ANG II type 2 receptor in the microcirculation of skeletal muscle. Am J Physiol. . 1998; 275: H1395–H1403.
6. Tsutusmi Y, Matsubara H, Masaki H, et al. Angiotensin II type 2 receptor overexpression activates the vascular kinin system and causes vasodilation. J Clin Invest. . 1999; 104: 925–935.[Medline] [Order article via Infotrieve]
7. Siragy HM, Carey RM. The subtype 2 (AT₂) angiotensin receptor mediates renal production of nitric oxide in conscious rats. J Clin Invest. . 1997; 100: 264–269.[Medline] [Order article via Infotrieve]
8. Stoll M, Steckelings UM, Paul M, Bottari , SP, Metzger R, Unger T. The angiotensin AT₂-receptor mediates inhibition of cell proliferation in coronary endothelial cells. J Clin Invest. . 1995; 95: 651–657.
9. Booz GW, Baker KM. Role of type 1 and type 2 angiotensin receptors in angiotensin II–induced cardiomyocyte hypertrophy. Hypertension. . 1996; 28: 635–640.[Abstract/Free Full Text]
10. Meffert S, Stoll M, Steckelings UM, Bottari SP, Unger T. The angiotensin II type 2 receptor inhibits proliferation and promotes differentiation in PC12W cells. Mol Cell Endocrinol. . 1996; 122: 59–67.[Medline] [Order article via Infotrieve]
11. Horiuchi M, Hayashida W, Kambe T, et al. Angiotensin type 2 receptor dephosphorylates Bcl-2 by activating mitogen-activated protein-kinase phosphatase-1 and induces apoptosis. J Biol Chem. . 1997; 272: 19022–19026.[Abstract/Free Full Text]
12. Tsutsumi Y, Ohkubo N, Iwasaka T, et al. Angiotensin II type 2 receptor is upregulated in human heart with interstitial fibrosis and cardiac fibroblasts are the major cell type for its expression. Circ Res. . 1998; 83: 1035–1046.[Abstract/Free Full Text]
13. Bedecs K, Elbaz N, Sutren M, et al. Angiotensin II type 2 receptors mediate inhibition of mitogen-activated protein kinase cascade and functional activation of SHP-1 tyrosine phosphatase. Biochem J. . 1997; 325: 449–454.
14. Huang XC, Richards EM, Sumners C. Mitogen-activated protein kinases in rat brain neuronal cultures are activated by angiotensin II type1 receptors and inhibited by angiotensin II type 2 receptors. J Biol Chem. . 1996; 271: 15635–15641.[Abstract/Free Full Text]
15. Murasawa S, Matsubara H, Mori Y, et al. Angiotensin II type 1 receptor–induced extracellular signal-regulated protein kinase activation is mediated by Ca²⁺/calmodulin-dependent transactivation of epidermal growth factor receptor. Circ Res. . 1998; 82: 1338–1348.[Abstract/Free Full Text]
16. Eguchi S, Numaguchi K, Iwasaki H, et al. Calcium-dependent epidermal growth factor receptor transactivation mediates the angiotensin II–induced mitogen-activated protein kinase activation in vascular smooth muscle cells. J Biol Chem. . 1998; 273: 8890–8896.[Abstract/Free Full Text]
17. Li X, Lee JW, Graves LM, Earp HS. Angiotensin II stimulates ERK via two pathways in epithelial cells: protein kinase C suppresses a G-protein– coupled receptor-EGF receptor transactivation pathway. EMBO J. . 1998; 17: 2574–2583.[Medline] [Order article via Infotrieve]
18. Yu H, Li X, Marchetto GS, et al. Activation of a novel calcium-dependent protein-tyrosine kinase: correlation with c-Jun N-terminal kinase but not mitogen-activated protein kinase activation. J Biol Chem. . 1996; 271: 29993–29998.[Abstract/Free Full Text]
19. Murasawa S, Matsubara H, Mori Y, et al. Angiotensin II initiates tyrosine kinase Pyk2-dependent signalings leading to activation of Rac1-mediated c-Jun NH₂-terminal kinase. J Biol Chem. . 2000; 275: 26856–26863.[Abstract/Free Full Text]
20. Lehtonen JYA, Daviet L, Nahmias C, Horinchi M, Dzau VJ. Analysis of functional domains of angiotensin II type 2 receptor involved in apoptosis. Mol Endocrinol. . 1999; 13: 1051–1060.[Abstract/Free Full Text]
21. Horiuchi M, Hayashida W, Akishita M, et al. Stimulation of different subtypes of angiotensin II receptors, AT₁ and AT₂ receptors, regulates STAT activation by negative crosstalk. Circ Res. . 1999; 84: 876–882.[Abstract/Free Full Text]
22. Elbaz N, Bedecs K, Masson M, Sutren M, Strosberg AD, Nahmias C. Functional trans-inactivation of insulin receptor kinase by growth-inhibitory angiotensin II AT₂ receptor. Mol Endocrinol. . 2000; 14: 795–804.[Abstract/Free Full Text]
23. Murasawa S, Shibasaki Y, Iwasaka T, et al. Role of calcium-sensitive tyrosine kinase Pyk2/CAKss/RAFTK in angiotensin II–induced Ras/ERK signaling. Hypertension. 1998; 32: 668–675.[Abstract/Free Full Text]
24. Moriguchi Y, Matsubara H, Mori M, et al. Angiotensin II–induced transactivation of EGF receptor regulates fibronectin and TGF-ß synthesis via transcription and post-transcriptional mechanisms. Circ Res. . 1999; 84: 1073–1084.[Abstract/Free Full Text]
25. Klingmuller U, Lorenz U, Cantlet LC, et al. Specific recruitment of SH-PTP1 to the erythropoietin receptor causes inactivation of JAK2 and termination of proliferative signals. Cell. . 1995; 80: 729–738.[Medline] [Order article via Infotrieve]
26. Vogel W, Lammers R, Huang J, et al. Activation of a phosphotyrosine phosphatase by tyrosine phosphorylation. Science. 1993; 259: 1611–1614.[Abstract/Free Full Text]
27. Tomic S, Greisert U, Lammers R, et al. Association of SH2 domain protein tyrosine phosphatase with the epidermal growth factor receptor in human tumor cells. J Biol Chem. . 1995; 270: 21277–21284.[Abstract/Free Full Text]
28. Su L, Zhao Z, Bouchard P, et al. Positive effect of overexpressed protein-tyrosine phosphatase PTP1C on mitogen-activated signaling in 293 cells. J Biol Chem. . 1996; 271: 10385–10390.[Abstract/Free Full Text]
29. Li RY, Gaits F, Ragab A, et al. Tyrosine phosphorylation of an SH2-containing protein tyrosine phosphatase is coupled to thrombin receptor via pertussis toxin-sensitive heterotrimeric G-protein. EMBO J. . 1995; 14: 2519–2526.[Medline] [Order article via Infotrieve]
30. Pei DH, Lorenz U, Neel BG, Walsh CT. Intramolecular regulation of protein tyrosine phosphatase SH-PTP1: a new function for Src homology 2 domains. Biochemistry. . 1994; 33: 15483–15493.[Medline] [Order article via Infotrieve]
31. Shultz LD, Schweitzer PA, Rajan TV, et al. Mutations at the murine motheaten locus are within the hematopoietic cell protein-tyrosine phosphatase (Hcph) gene. Cell. . 1993; 73: 1445–1454.[Medline] [Order article via Infotrieve]
32. Tang H, Zhao ZJ, Landon EJ, Inagami T. Regulation of calcium-sensitive tyrosine kinase Pyk2 by angiotensin II in endothelial cells. J Biol Chem. . 2000; 275: 8389–8396.[Abstract/Free Full Text]
33. Ganju RK, Brubaker SA, Chernock RD, Avraham S, Groopman JE. Beta-chemokine receptor CCR5 signals through SHP-1, SHP-2 and Syk. J Biol Chem. . 2000; 275: 17263–17268.[Abstract/Free Full Text]
34. Ochi F, Matozaki T, Okabayashi Y, et al. Epidermal growth factor stimulates tyrosine phosphorylation of SHPS-1 and association of SHPS-1 with SHPS-2, a SH2 domain-containing protein tyrosine phosphatase. Biochem Biophys Res Commun. . 1997; 239: 483–487.[Medline] [Order article via Infotrieve]
35. Marrero MB, Venema VJ, Ju H, Eaton DC, Venema RC. Regulation of angiotensin II–induced JAK2 tyrosine phosphorylation: roles of SHP-1 and SHP-2. Am J Physiol. . 1998; 275: C1216–C1223.
36. Berk BC, Corson MA. Angiotensin II signal transduction in vascular smooth muscle: role of tyrosine kinases. Circ Res. . 1997; 80: 607–616.[Abstract/Free Full Text]
37. Yamada T, Horiuchi M, Dzau VJ. The novel angiotensin TT type 2 receptor mediates programmed cell death. Proc Natl Acad Sci U S A. . 1996; 93: 156–160.[Abstract/Free Full Text]

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