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Hypertension. 2003;42:664-668
Published online before print July 21, 2003, doi: 10.1161/01.HYP.0000084370.74777.B6
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(Hypertension. 2003;42:664.)
© 2003 American Heart Association, Inc.


State of the Art Lectures

Peroxisome Proliferator-Activated Receptors

Vascular and Cardiac Effects in Hypertension

Ernesto L. Schiffrin; Farhad Amiri; Karim Benkirane; Marc Iglarz; Quy N. Diep

From CIHR Multidisciplinary Research Group on Hypertension, Clinical Research Institute of Montreal, Montreal, Quebec, Canada.

Correspondence to Ernesto L. Schiffrin, MD, Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal, Quebec, Canada H2W 1R7. E-mail ernesto.schiffrin{at}ircm.qc.ca


*    Abstract
up arrowTop
*Abstract
down arrowIntroduction
down arrowVascular Effects of PPARs
down arrowCardiac Effects of PPARs
down arrowPerspectives
down arrowReferences
 
Peroxisome proliferator-activated receptors (PPAR) are nuclear receptors acting as transcription factors on numerous target genes after heterodimerization with the retinoid X receptor. PPAR-{alpha} and PPAR-{gamma} may be activated by different agonists, although the endogenous ligands are unknown. Although PPAR-{alpha} is mainly involved in fatty acid oxidation and expressed in liver, kidney, and skeletal muscle, and PPAR-{gamma} is mainly involved in fat cell differentiation and insulin sensitivity, both are expressed in smooth muscle cells and myocardium, although PPAR-{gamma} are scarce in the latter. Activators of PPAR-{alpha} such as fatty acids and fibrates, and PPAR-{gamma} such as thiazolidinediones have been shown to exert antiproliferative effects, antagonize angiotensin II actions in vivo and in vitro, and exert antioxidant actions inhibiting generation of reactive oxygen species and activation of inflammatory mediators on blood vessels and the heart. These agents lowered blood pressure in several models of hypertension and corrected endothelial dysfunction. They exerted anti-inflammatory and antifibrotic actions on blood vessels and the heart. With the development of dual {alpha}/{gamma}-PPAR activators, these newer agents may become interesting therapeutic agents for prevention of vascular and cardiac complications of hypertension as well as for preventative therapy in other forms of cardiovascular disease.


Key Words: cardiovascular diseases • transcription • arteries • vasculature • remodeling • heart • fibrosis


*    Introduction
up arrowTop
up arrowAbstract
*Introduction
down arrowVascular Effects of PPARs
down arrowCardiac Effects of PPARs
down arrowPerspectives
down arrowReferences
 
Peroxisome proliferator-activated receptors (PPARs)1 are nuclear factors discovered for their ability to respond to xenobiotics with peroxisomal proliferation in the liver of rodents. They are encoded by 3 distinct genes: {alpha}, ß/{delta}, and {gamma}. Initially believed to regulate genes involved only in lipid and glucose metabolism, the role of PPARs has more recently been increasingly associated with regulation of cell growth and migration2 and inflammation.3 PPAR-{alpha} may be activated by fatty acids, fibrates, and leukotriene B4 to induce transcription of genes involved in {omega}- and ß-oxidation of fatty acids. PPAR-{alpha} is mainly expressed in tissues in which fatty acid catabolism is important, such as liver, kidney, heart, and muscles. Shortly after the discovery of PPAR-{alpha}, PPAR-ß/{delta}, and PPAR-{gamma} were identified.4 PPAR-ß/{delta} is expressed ubiquitously,5,6 and its function remains unclear, although recent evidence suggests a role on fatty acid and lipid metabolism,7 particularly in the heart.8 PPAR-{gamma} is highly expressed in adipose tissue, where it controls adipocyte differentiation and lipid storage9 and modulates the action of insulin. The Figure shows schematically the activation of PPARs and their transcriptional role.



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Scheme shows activation of PPARs and their binding to PPAR response elements (PPRE) as a heterodimer with the retinoid X receptor (RXR). Classic metabolic effects attributable to PPAR-{alpha} and PPAR-{gamma}, as well as the cardiovascular actions such as the anti-inflammatory, antifibrotic actions and antihypertrophic effects more recently demonstrated, are shown. Effects of PPAR-ß/{delta} remain unclear, although action on lipid metabolism and the heart similar to PPAR-{alpha} has recently been reported.7,8

PPAR structure includes an N-terminal domain that regulates PPAR activity, a DNA-binding domain that binds to the PPAR response element (PPRE) in the promoter region of target genes, a domain for a cofactor, and a C-terminal ligand-binding domain. Ligand specificity is determined by the latter.10 When activators bind to PPARs, they heterodimerize with retinoid X receptors (RXR-{alpha}) and then may bind to PPRE in target genes to modulate gene transcription (Figure).11 In the inactivated state, PPARs are bound to corepressor proteins. Under the effect of PPAR activators, PPARs dissociate from corepressors and recruit coactivators, including a PPAR-binding protein and the steroid receptor coactivator-1.12

PPAR-{alpha} is activated by natural ligands such as fatty acids and eicosanoids and by synthetic ligands, the lipid-lowering fibrates.13 Selective activators of PPAR-{gamma} are the insulin sensitizers thiazolidinedione glitazones, such as troglitazone, pioglitazone, and rosiglitazone (Figure).


*    Vascular Effects of PPARs
up arrowTop
up arrowAbstract
up arrowIntroduction
*Vascular Effects of PPARs
down arrowCardiac Effects of PPARs
down arrowPerspectives
down arrowReferences
 
Since both PPAR-{alpha} and PPAR-{gamma} are expressed in the cardiovascular system,14 such as in endothelial cells15,16 vascular smooth muscle cells (VSMC)17 and monocytes/macrophages,18,19 a number of studies have been carried out to elucidate the cellular and molecular mechanisms underlying PPAR actions on the vasculature. We first identified that the PPAR-{alpha} ligand docosahexanoic acid (DHA) had proapoptotic effects on cultured VSMCs.20 This proapoptotic action was mediated by activation of p38 mitogen–activated protein kinase.21 PPAR-{alpha} ligands inhibited IL-1ß–induced production of interleukin (IL)-6 and prostaglandin and expression of cyclooxygenase-2, as a result of PPAR-{alpha} repression of transcription factor nuclear factor-{kappa}B (NF-{kappa}B) signaling.18 The PPAR-{alpha} activator fenofibrate significantly reduces plasma interferon-{gamma} and tumor necrosis factor-{alpha} (TNF-{alpha}) in patients with hyperlipoproteinemia IIb,22 demonstrating its anti-inflammatory activity. PPAR-{alpha} activators also downregulate cytokine-induced genes, such as expression of vascular cell adhesion molecule (VCAM)-1 and tissue factor in endothelial cells.23 PPAR-{alpha}–deficient mice had exaggerated inflammatory response to lipopolysaccharide (LPS) stimulation, and fibrates failed to affect LPS-induced IL-6 transcription in these mice.24 The molecular mechanisms of the anti-inflammatory action of PPAR-{alpha} activators could involve antagonism of the NF-{kappa}B signaling pathway.23–25 Accordingly, we investigated the effect of the PPAR-{alpha} activator DHA in angiotensin (Ang) II–infused rats and demonstrated that the PPAR-{alpha} activator reduced Ang II–induced oxidative stress and inflammatory mediators in blood vessels.26 Systolic blood pressure elevated in Ang II–infused rats was reduced by DHA from 172±3 to 112±4 mm Hg (P<0.01). In mesenteric small arteries studied in a pressurized myograph, media/lumen ratio was increased and acetylcholine-induced relaxation impaired in Ang II–infused rats; both were normalized by DHA. NADPH oxidase activity measured by chemiluminescence and expression of adhesion molecules intercellular adhesion molecule (ICAM) and VCAM-1 were significantly increased in blood vessels of Ang II–infused rats, changes that were abrogated by DHA. Thus, DHA attenuated the development of hypertension, corrected structural abnormalities, and improved endothelial dysfunction induced by Ang II. These effects were associated with decreased oxidative stress and inflammation in the vascular wall.

PPAR-{gamma} as mentioned is involved in adipocyte differentiation and insulin sensitivity. However, it has been shown to be expressed in smooth muscle27,28 and in monocytes/macrophages.19 PPAR-{gamma} inhibits proliferation and migration of VSMCs.2,27 PPAR-{gamma} is upregulated in activated macrophages and inhibits the expression of inducible nitric oxide synthase (iNOS), matrix metalloproteinase (MMP)-9, and scavenger receptor A genes in response to 15-deoxy-({delta}-12-14)-prostaglandin J2 (15d-PGJ2) and synthetic PPAR-{gamma} ligands. PPAR-{gamma} activation inhibits gene expression in part by antagonizing the activities of these transcription factors: activator protein 1 (AP-1), signal transduction-activated transcription factors (STAT), and NF-{kappa}B. In monocytes, PPAR-{gamma} activators inhibit the expression of TNF-{alpha}, IL-6, IL-1ß,29 iNOS, MMP-9, and scavenger receptor A in monocytes.30 PPAR-{gamma} expression has been demonstrated in atherosclerotic plaques31 and in endothelial cells15,16 whose function is altered in atherosclerosis, where PPAR-{gamma} could play an antiatheroslcerotic role. The PPAR-{gamma} activators, troglitazone and 15d-PGJ2, attenuated TNF-induced VCAM-1 and ICAM-1 expression in endothelial cells, and troglitazone reduced monocyte/macrophage homing to atherosclerotic plaques in apoE-deficient mice.32 However, 15d-PGJ2 may stimulate the synthesis of IL-8 in endothelial cells in a PPAR-{gamma}–independent manner.33 The mechanism of the anti-inflammatory effect may depend on interactions with different signaling pathways. One recently demonstrated is interaction with CCAAT/enhancer-binding protein (C/EBP)-{delta}, which is present in tandem repeats in the PPAR-{gamma} gene promoter and upregulates transcription of inflammatory cytokines. The latter are negatively autoregulated by PPAR-{gamma} in the vasculature.34 PPAR-{gamma} ligands troglitazone, pioglitazone, and 15d-PGJ2 transcriptionally inhibited IL-1ß–induced IL-6 expression in VSMCs. Thus C/EBP-{delta} may be negatively autoregulated through transactivation of PPAR-{gamma}, downregulating inflammatory responses. PPAR-{gamma} may also play an anti-inflammatory role in hypertensive models, such as Ang II–induced hypertension.

We recently demonstrated that the PPAR-{gamma} activators rosiglitazone and pioglitazone prevented hypertension in Ang II–infused rats and abrogated the structural, functional, and molecular changes induced by Ang II in blood vessels by exerting direct effects on the vascular wall, leading to inhibition of cell growth and inflammation.35 In mesenteric small arteries studied in a pressurized myograph, media/lumen ratio was increased and acetylcholine-induced relaxation impaired in Ang II–infused rats; both were normalized by the thiazolidinediones. In Ang II–infused rats, vascular DNA synthesis (by 3H-thymidine incorporation); expression of cell cycle proteins cyclin D1 and cdk4, Ang II type 1 (AT1) receptors, vascular VCAM-1, and platelet and endothelial cell adhesion molecule (PECAM); and NF-{kappa}B activity were increased. These changes were abrogated by pioglitazone or rosiglitazone.

PPARs may also modulate in vitro the vascular production of vasoactive peptides such as endothelin-1 (ET-1). We investigated the in vivo interaction between PPARs and ET-1 in DOCA-salt rats, which overexpress vascular ET-1.36 Blood pressure increase was partially prevented in the DOCA-salt hypertensive rats by coadministration of the PPAR-{gamma} activator rosiglitazone but not by the PPAR-{alpha} activator fenofibrate. Both PPAR activators abrogated the increase of preproET-1 mRNA content in the mesenteric vasculature of DOCA-salt rats. Rosiglitazone and fenofibrate prevented the hypertrophic remodeling in DOCA-salt rats but did not affect vessel mechanics. Rosiglitazone but not fenofibrate prevented endothelial dysfunction. Furthermore, both rosiglitazone and fenofibrate prevented the vascular increase of superoxide anion production found in DOCA-salt animals.

Spontaneously hypertensive rats (SHR) have insulin resistance that has been associated with a mutation of cd36, which encodes for a fatty acid translocase, and results in decreased fatty acid translocation.37 cd36 is a target of PPAR-{gamma}. We therefore hypothesized that there could be changes in expression of PPARs in blood vessels of SHR that could result in decreased inhibition of proliferation, migration, inflammation, and fibrosis in this hypertensive model. However, when this hypothesis was tested, we found the opposite, that is, increased rather than decreased expression of PPAR-{alpha} and PPAR-{gamma} in blood vessels and in cultured VSMC from SHR28 We interpret this as a possible compensatory (feedback?) response to the decreased activity of the mutant cd36 of SHR.


*    Cardiac Effects of PPARs
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowVascular Effects of PPARs
*Cardiac Effects of PPARs
down arrowPerspectives
down arrowReferences
 
PPAR-{alpha} plays an important role in regulation of energy and lipid metabolism and accordingly in the pathophysiology of heart disease. PPAR-{alpha} is involved in mitochondrial fatty acid ß-oxidation, a critical fuel-generating mechanism of the heart.38 PPAR-{alpha} controls myocardial lipid metabolism through the activation of transcription of the muscle carnitine palmitoyltransferase I (CPT I) gene.38 It serves as a molecular "lipostat" by inducing the expression of target genes involved in cardiac fatty acid metabolism.39 The capacity for constitutive myocardial ß-oxidation of the medium and long chain fatty acids octanoic acid and palmitic acid was markedly reduced in the PPAR-{alpha}–null mice compared with wild-type mice,40 indicating that mitochondrial fatty acid catabolism is impaired in the absence of PPAR-{alpha}. In contrast, constitutive ß-oxidation of the very long chain fatty acid lignoceric acid did not differ between the PPAR-{alpha}–deficient mice and the wild type, which suggests that the constitutive expression of enzymes involved in peroxisomal ß-oxidation is independent of PPAR-{alpha}.

PPAR-{alpha} is deactivated during cardiac hypertrophy.41 Hypertrophied myocytes accumulate fat intracellularly in response to oleate loading, which indicates that PPAR-{alpha} deactivation reduces the capacity for myocardial lipid and energy homeostasis. PPAR-{alpha} also exerts anti-inflammatory action on the heart. PPAR-{alpha} activators inhibit cardiac expression of TNF-{alpha} and NF-{kappa}B induced by lipopolysaccharide.42 The PPAR-{alpha} activator fenofibrate reduced preproET-1 mRNA expression and collagen type I and type III mRNA, associated with decrease in interstitial and perivascular cardiac fibrosis after pressure overload induced by abdominal aortic banding,43 probably through suppression of AP-1–mediated preproET-1 gene activation. Additionally, fenofibrate reduced cardiac hypertrophy and inflammation associated with an increase in the anti-inflammatory cytokine IL-10.44 We recently observed that fenofibrate had beneficial effects on inflammation and collagen deposition in the heart of Ang II–infused rats.45 This was associated with a decrease in NF-{kappa}B activity, VCAM-1, PECAM, ICAM-1- and ED-1 (macrophage antigen) expression and downregulation of AT1 and upregulation of AT2 receptors.

The role of PPAR-{gamma} in the heart remains unclear. This is complicated by the fact that expression of PPAR-{gamma} in the heart is very low.46 PPAR-{gamma} may act as an inhibitor of cardiac hypertrophy. Both troglitazone and the endogenous PPAR-{gamma} ligand 15d-PGJ2 blocked hypertrophy and brain natriuretic peptide expression in cultured cardiomyocytes.47 PPAR-{gamma} may function as a transducer of antihypertrophic signaling in the heart. In heterozygous PPAR-{gamma}–deficient mice, an exaggerated hypertrophic response to pressure overload induced by aortic banding was noted.48 In contrast, pioglitazone significantly blunted myocardial hypertrophy in both wild-type and PPAR-{gamma} -/- mice, although to varying degrees. Ang II–induced hypertrophic gene expression, as well increased cardiomyocyte size, may be attenuated in vitro by thiazolidinediones. Taken together, these data suggest that PPAR-{gamma} negatively influences cardiac hypertrophy. In addition, PPAR-{gamma} improved left ventricular diastolic function and decreased collagen accumulation in diabetic rats49,50 and protected myocardium from ischemic injury.51,52 However, clinical reports have recently warned that PPAR-{gamma} activator glitazones may lead to development or exacerbate congestive heart failure.53 Among molecular adaptations of the hypertrophic heart is an increase in glucose utilization and decreased fatty acid oxidation. Whether or not PPAR-{gamma} has similar regulating effects on fatty acid metabolism as PPAR-{alpha} is unclear. Since both PPAR-{alpha} and PPAR-{gamma} have a partially overlapping ligand binding profile, PPAR-{gamma} could mediate to some degree similar signals as PPAR-{alpha} in cardiomyocytes. PPAR-{gamma} signaling could attenuate cardiac remodeling through pathways not directly involved in controlling lipid and energy metabolism, such as inflammation. Inflammation is an important mechanism in the progression of cardiac remodeling and dysfunction. In macrophages, PPAR-{gamma} is involved in regulation of inflammatory responses by antagonism of transcription factors NF-{kappa}B and AP-1.19 NF-{kappa}B is required for the hypertrophic response of neonatal rat cardiomyocytes in vitro.54 Indeed, we recently observed that the PPAR-{gamma} activator pioglitazone had beneficial long-term effects on cardiac hypertrophy and cardiac inflammation without affecting cardiac function in stroke-prone SHR.55 However, whether PPAR-{gamma} effects on the heart are directly exerted on cardiomyocytes or occur through infiltrating macrophages and other blood-borne cells or are the result of endocrine actions mediated indirectly by other organs through hormonal effects remains unclear.46


*    Perspectives
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowVascular Effects of PPARs
up arrowCardiac Effects of PPARs
*Perspectives
down arrowReferences
 
Based on in vivo and in vitro studies with different cell types, it is clear that PPAR-{alpha} and PPAR-{gamma} play an important role in the modulation of inflammatory, fibrotic and hypertrophic responses (Figure). However, our knowledge of the regulatory mechanisms and signaling cascades underlying the anti-inflammatory effect of PPARs, particularly in the heart, is still limited. More research in this area is required. In addition, the discrepancy between the beneficial effect of PPAR-{gamma} activators on the heart in experimental models and clinical reports of heart failure in a few diabetic patients treated with PPAR-{gamma} activators needs to be further clarified. It is likely that in those patients, water and salt retention induced by the insulin-sensitizing action of PPAR-{gamma} activators unmasks a latent left ventricular dysfunction and may thus precipitate cardiac failure that is not induced directly by PPAR-{gamma} activators.56

Both PPAR-{alpha} and PPAR-{gamma} activators may interfere with signaling pathways, leading to cardiovascular damage, inflammation, fibrosis, and growth. Use of selective PPAR-{alpha} or PPAR-{gamma} activators or dual {alpha}/{gamma} activators may exert cardiovascular protective effects in hypertension or other forms of cardiovascular disease.


*    Acknowledgments
 
Work from our laboratory was supported by grants 13570 and 37917 and a group grant to the Multidisciplinary Research Group on Hypertension (E.L.S.) and a postdoctoral fellowship (Q.N.D.), all from Canadian Institutes of Health Research (CIHR).

Received May 5, 2003; first decision May 27, 2003; accepted June 19, 2003.


*    References
up arrowTop
up arrowAbstract
up arrowIntroduction
up arrowVascular Effects of PPARs
up arrowCardiac Effects of PPARs
up arrowPerspectives
*References
 
1. Issemann I, Green S. Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators. Nature. 1990; 347: 645–650.[CrossRef][Medline] [Order article via Infotrieve]

2. Law RE, Hsueh WA. PPAR{gamma} and atherosclerosis: effects on cell growth and movement. Arterioscler Thromb Vasc Biol. 2001; 21: 1891–1895.[Abstract/Free Full Text]

3. Delerive P, Fruchart C, Staels B. Peroxisome proliferator-activated receptors in inflammation control. J Endocrinol. 2001; 169: 453–459.[Abstract]

4. Dreyer C, Krey G, Keller H, Givel F, Helftenbein G, Wahli W. Control of the peroxisomal ß-oxidation pathway by a novel family of nuclear hormone receptors. Cell. 1992; 68: 879–887.[CrossRef][Medline] [Order article via Infotrieve]

5. Kliewer SA, Forman BM, Blumberg B, Ong ES, Borgmeyer U, Mangelsdorf DJ, Umesono K, Evans RM. Differential expression and activation of a family of murine peroxisome proliferator-activated receptors. Proc Natl Acad Sci U S A. 1994; 91: 7355–7359.[Abstract/Free Full Text]

6. Braissant O, Foufelle F, Scotto C, Dauca M, Wahli W. Differential expression of peroxisome proliferator-activated receptors (PPARs): tissue distribution of PPAR-{alpha}, -ß, and -{gamma} in the adult rat. Endocrinology. 1996; 137: 354–366.[Abstract]

7. Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblasts by PPAR{gamma}2, a lipid-activated transcription factor. Cell. 1994; 79: 1147–1156.[CrossRef][Medline] [Order article via Infotrieve]

8. Gilde AJ, van der Lee KAJM, Willemsen PHM, Chinetti G, van der Leij FR, van der Vusse GJ, Staels B, van Bilsen M. Peroxisome proliferators activated receptor (PPAR) {alpha} and PPARß/{delta}, but not PPAR{gamma}, modulate the expression of genes involved in cardiac lipid metabolism. Circ Res. 2003; 92: 518–524.[Abstract/Free Full Text]

9. Chawla A, Lee CH, Barak Y, He W, Rosenfeld J, Liao D, Han J, Kang H, Evans RM. PPAR{delta} is a very low-density lipoprotein sensor in macrophages. Proc Natl Acad Sci U S A. 2003; 100: 1268–1273.[Abstract/Free Full Text]

10. Tugwood JD, Issemann I, Anderson RG, Bundell KR, McPheat WL, Green S. The mouse peroxisome proliferator activated receptor recognizes a response element in the 5' flanking sequence of the rat acyl CoA oxidase gene. EMBO J. 1992; 11: 433–439.[Medline] [Order article via Infotrieve]

11. Ijpenberg A, Jeannin E, Wahli W, Desvergne B. Polarity and specific sequence requirements of peroxisome proliferator-activated receptor (PPAR)/retinoid X receptor heterodimer binding to DNA: a functional analysis of the malic enzyme gene PPAR response element. J Biol Chem. 1997; 272: 20108–20117.[Abstract/Free Full Text]

12. Llopis J, Westin S, Ricote M, Wang Z, Cho CY, Kurokawa R, Mullen TM, Rose DW, Rosenfeld MG, Tsien RY, Glass CK, Wang J. Ligand-dependent interactions of coactivators steroid receptor coactivator-1 and peroxisome proliferator-activated receptor binding protein with nuclear hormone receptors can be imaged in live cells and are required for transcription. Proc Natl Acad Sci U S A. 2000; 97: 4363–4368.[Abstract/Free Full Text]

13. Desvergne B, Wahli W. Peroxisome proliferator-activated receptors: nuclear control of metabolism. Endocr Rev. 1999; 20: 649–688.[Abstract/Free Full Text]

14. Bishop-Bailey D. Peroxisome proliferator-activated receptors in the cardiovascular system. Br J Pharmacol. 2000; 129: 823–834.[CrossRef][Medline] [Order article via Infotrieve]

15. Inoue I, Shino K, Noji S, Awata T, Katayama S. Expression of peroxisome proliferator-activated receptor {alpha} (PPAR{alpha}) in primary cultures of human vascular endothelial cells. Biochem Biophys Res Commun. 1998; 246: 370–374.[CrossRef][Medline] [Order article via Infotrieve]

16. Satoh H, Tsukamoto K, Hashimoto Y, Hashimoto N, Togo M, Hara M, Maekawa H, Isoo N, Kimura S, Watanabe T. Thiazolidinediones suppress endothelin-1 secretion from bovine vascular endothelial cells: a new possible role of PPAR{gamma} on vascular endothelial function. Biochem Biophys Res Commun. 1999; 254: 757–763.[CrossRef][Medline] [Order article via Infotrieve]

17. Staels B, Koenig W, Habib A, Merval R, Lebret M, Torra IP, Delerive P, Fadel A, Chinetti G, Fruchart JC, Najib J, Maclouf J, Tedgui A. Activation of human aortic smooth-muscle cells is inhibited by PPAR{alpha} but not by PPAR{gamma} activators. Nature. 1998; 393: 790–793.[CrossRef][Medline] [Order article via Infotrieve]

18. Chinetti G, Griglio S, Antonucci M, Torra IP, Delerive P, Majd Z, Fruchart JC, Chapman J, Najib J, Staels B. Activation of proliferator-activated receptors {alpha} and {gamma} induces apoptosis of human monocyte-derived macrophages. J Biol Chem. 1998; 273: 25573–25580.[Abstract/Free Full Text]

19. Ricote M, Li AC, Willson TM, Kelly CJ, Glass CK. The peroxisome proliferator-activated receptor-{gamma} is a negative regulator of macrophage activation. Nature. 1998; 391: 79–82.[CrossRef][Medline] [Order article via Infotrieve]

20. Diep QN, Intengan HD, Schiffrin EL. Endothelin-1 attenuates {omega}-3 fatty acid-induced apoptosis by inhibition of caspase 3. Hypertension. 2000; 35: 287–291.[Abstract/Free Full Text]

21. Diep QN, Touyz RM, Schiffrin EL. Docosahexaenoic acid, a peroxisome proliferator-activated receptor-{alpha} activator, induces apoptosis in vascular smooth muscle cells by activation of p38 mitogen-activated protein kinase. Hypertension. 2000; 36: 851–855.[Abstract/Free Full Text]

22. Madej A, Okopien B, Kowalski J, Zielinski M, Wysocki J, Szygula B, Kalina Z, Herman ZS. Effects of fenofibrate on plasma cytokine concentrations in patients with atherosclerosis and hyperlipoproteinemia IIb. Int J Clin Pharmacol Ther. 1998; 36: 345–349.[Medline] [Order article via Infotrieve]

23. Marx N, Sukhova GK, Collins T, Libby P, Plutzky J. PPAR{alpha} activators inhibit cytokine-induced vascular cell adhesion molecule-1 expression in human endothelial cells. Circulation. 1999; 99: 3125–3131.[Abstract/Free Full Text]

24. Delerive P, De Bosscher K, Besnard S, Vanden Berghe W, Peters JM, Gonzalez FJ, Fruchart JC, Tedgui A, Haegeman G, Staels B. Peroxisome proliferator-activated receptor {alpha} negatively regulates the vascular inflammatory gene response by negative cross-talk with transcription factors NF-{kappa}B and AP-1. J Biol Chem. 1999; 274: 32048–32054.[Abstract/Free Full Text]

25. Poynter ME, Daynes RA. Peroxisome proliferator-activated receptor {alpha} activation modulates cellular redox status, represses nuclear factor-{kappa}B signaling, and reduces inflammatory cytokine production in aging. J Biol Chem. 1998; 273: 32833–32841.[Abstract/Free Full Text]

26. Diep QN, Amiri F, Touyz RM, Cohn JS. Endemann D, Schiffrin EL. PPAR{alpha} activator effects on Ang II-induced vascular oxidative stress and inflammation. Hypertension. 2002; 40: 866–871.[Abstract/Free Full Text]

27. Law RE, Goetze S, Xi X-P, Jackson S, Kawano Y, Demer L, Fishbein MC, Meehan WP, Hsueh WA. Expression and function of PPAR{gamma} in rat and human vascular smooth muscle cells. Circulation. 2000; 101: 1311–1318.[Abstract/Free Full Text]

28. Diep QN, Schiffrin EL. Increased expression of peroxisome proliferator-activated receptor-{alpha} and -{gamma} in blood vessels of spontaneously hypertensive rats. Hypertension. 2001; 38: 249–254.[Abstract/Free Full Text]

29. Jiang C, Ting AT, Seed B. PPAR-{gamma} agonists inhibit production of monocyte inflammatory cytokines. Nature. 1998; 391: 82–86.[CrossRef][Medline] [Order article via Infotrieve]

30. Ricote M, Huang JT, Welch JS, Glass CK. The peroxisome proliferator-activated receptor{gamma} (PPAR{gamma}) as a regulator of monocyte/macrophage function. J Leukoc Biol. 1999; 66: 733–739.[Abstract]

31. Ricote M, Huang J, Fajas L, Li A, Welch J, Najib J, Witztum JL, Auwerx J, Palinski W, Glass CK. Expression of the peroxisome proliferator-activated receptor{gamma} (PPAR{gamma}) in human atherosclerosis and regulation in macrophages by colony stimulating factors and oxidized low density lipoprotein. Proc Natl Acad Sci U S A. 1998; 95: 7614–7619.[Abstract/Free Full Text]

32. Pasceri V, Wu HD, Willerson JT, Yeh ET. Modulation of vascular inflammation in vitro and in vivo by peroxisome proliferator-activated receptor-{gamma} activators. Circulation. 2000; 101: 235–238.[Abstract/Free Full Text]

33. Jozkowicz A, Dulak J, Prager M, Nanobashvili J, Nigisch A, Winter B, Weigel G, Huk I. Prostaglandin-J2 induces synthesis of interleukin-8 by endothelial cells in a PPAR-{gamma}-independent manner. Prostaglandins Other Lipid Mediat. 2001; 66: 165–177.[CrossRef][Medline] [Order article via Infotrieve]

34. Takata Y, Kitami Y, Yang ZH, Nakamura M, Okura T, Hiwada K. Vascular inflammation is negatively autoregulated by interaction between CCAAT/enhancer-binding protein-{delta} and peroxisome proliferator-activated receptor-{gamma}. Circ Res. 2002; 91: 427–433.[Abstract/Free Full Text]

35. Diep QN, El Mabrouk M, Cohn JS, Endemann D, Amiri F, Virdis A, Neves MF, Schiffrin EL. Structure, endothelial function, cell growth, and inflammation in blood vessels of angiotensin II-infused rats: role of peroxisome proliferator-activated receptor-{gamma}. Circulation. 2002; 105: 2296–2302.[Abstract/Free Full Text]

36. Iglarz M, Touyz RM, Amiri F, Lavoie MF, Diep QN, Schiffrin EL. Effect of peroxisome proliferator-activated receptor-{alpha} and -{gamma} activators on vascular remodeling in endothelin-dependent hypertension. Arterioscler Thromb Vasc Biol. 2003; 23: 45–51.[Abstract/Free Full Text]

37. Aitman TJ, Glazier AM, Wallace CA, Cooper LD, Norsworthy PJ, Wahid FN, Al-Majali KM, Trembling PM, Mann CJ, Shoulders CC, Graf D, St Lezin E, Kurtz TW, Kren V, Pravenec M, Ibrahimi A, Abumrad NA, Stanton LW, Scott J. Identification of Cd36 (Fat) as an insulin-resistance gene causing defective fatty acid and glucose metabolism in hypertensive rats. Nat Genet. 1999; 21: 76–83.[CrossRef][Medline] [Order article via Infotrieve]

38. Brandt JM, Djouadi F, Kelly DP. Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor {alpha}. J Biol Chem. 1998; 273: 23786–23792.[Abstract/Free Full Text]

39. Djouadi F, Brandt JM, Weinheimer CJ, Leone TC, Gonzalez FJ, Kelly DP. The role of the peroxisome proliferator-activated receptor {alpha} (PPAR {alpha}) in the control of cardiac lipid metabolism. Prostaglandins Leukot Essent Fatty Acids. 1999; 60: 339–343.[CrossRef][Medline] [Order article via Infotrieve]

40. Watanabe K, Fujii H, Takahashi T, Kodama M, Aizawa Y, Ohta Y, Ono T, Hasegawa G, Naito M, Nakajima T, Kamijo Y, Gonzalez FJ, Aoyama T. Constitutive regulation of cardiac fatty acid metabolism through peroxisome proliferator-activated receptor {alpha} associated with age-dependent cardiac toxicity. J Biol Chem. 2000; 275: 22293–22299.[Abstract/Free Full Text]

41. Barger PM, Brandt JM, Leone TC, Weinheimer CJ, Kelly DP. Deactivation of peroxisome proliferator-activated receptor-{alpha} during cardiac hypertrophic growth. J Clin Invest. 2000; 105: 1723–1730.[Medline] [Order article via Infotrieve]

42. Takano H, Nagai T, Asakawa M, Toyozaki T, Oka T, Komuro I, Saito T, Masuda Y. Peroxisome proliferator-activated receptor activators inhibit lipopolysaccharide-induced tumor necrosis factor-alpha expression in neonatal rat cardiac myocytes. Circ Res. 2000; 87: 596–602.[Abstract/Free Full Text]

43. Ogata T, Miyauchi T, Sakai S, Irukayama-Tomobe Y, Goto K, Yamaguchi I. Stimulation of peroxisome-proliferator-activated receptor {alpha} (PPAR{alpha}) attenuates cardiac fibrosis and endothelin-1 production in pressure-overloaded rat hearts. Clin Sci. 2002; 103 (suppl 1): 284S–288S[Medline] [Order article via Infotrieve]

44. Maruyama S, Kato K, Kodama M, Hirono S, Fuse K, Nakagawa O, Nakazawa M, Miida T, Yamamoto T, Watanabe K, Aizawa Y. Fenofibrate, a peroxisome proliferator-activated receptor alpha activator, suppresses experimental autoimmune myocarditis by stimulating the interleukin-10 pathway in rats. J Atheroscler Thromb. 2002; 9: 87–92.[Medline] [Order article via Infotrieve]

45. Diep QN, Benkirane K, Schiffrin EL. PPAR{alpha} activator fenofibrate inhibits myocardial fibrosis and inflammation in angiotensin II-infused rats. Hypertension. 2002; 40: 388. Abstract.

46. Kelly DP. PPARs of the heart. Circ Res. 2003; 92: 482–484.[Free Full Text]

47. Yamamoto K, Ohki R, Lee RT, Ikeda U, Shimada K. Peroxisome proliferator-activated receptor {gamma} activators inhibit cardiac hypertrophy in cardiac myocytes. Circulation. 2001; 104: 1670–1675.[Abstract/Free Full Text]

48. Asakawa M, Takano H, Nagai T, Uozumi H, Hasegawa H, Kubota N, Saito T, Masuda Y, Kadowaki T, Komuro IF. Peroxisome proliferator-activated receptor {gamma} plays a critical role in inhibition of cardiac hypertrophy in vitro and in vivo. Circulation. 2002; 105: 1240–1246.[Abstract/Free Full Text]

49. Tsuji T, Mizushige K, Noma T, Murakami K, Ohmori K, Miyatake A, Kohno M. Pioglitazone improves left ventricular diastolic function and decreases collagen accumulation in prediabetic stage of a type II diabetic rat. J Cardiovasc Pharmacol. 2001; 38: 868–874.[CrossRef][Medline] [Order article via Infotrieve]

50. Zhu P, Lu L, Xu Y, Schwartz GG. Troglitazone improves recovery of left ventricular function after regional ischemia in pigs. Circulation. 2000; 101: 1165–1171.[Abstract/Free Full Text]

51. Sidell RJ, Cole MA, Draper NJ, Desrois M, Buckingham RE, Clarke K. Thiazolidinedione treatment normalizes insulin resistance and ischemic injury in the Zucker fatty rat heart. Diabetes. 2002; 51: 1110–1117.[Abstract/Free Full Text]

52. Yue TL, Chen J, Bao W, Narayanan PK, Bril A, Jiang W, Lysko PG, Gu JL, Boyce R, Zimmerman DM, Hart TK, Buckingham RE, Ohlstein EH. In vivo myocardial protection from ischemia/reperfusion injury by the peroxisome proliferator-activated receptor-{gamma} agonist rosiglitazone. Circulation. 2001; 104: 2588–2594.[Abstract/Free Full Text]

53. Wooltorton E. Rosiglitazone (Avandia) and pioglitazone (Actos) and heart failure. Can Med Assoc J. 2002; 166: 219.[Free Full Text]

54. Purcell NH, Tang G, Yu C, Mercurio F, DiDonato JA, Lin A. Activation of NF-{kappa}B is required for hypertrophic growth of primary rat neonatal ventricular cardiomyocytes. Proc Natl Acad Sci U S A. 2001; 98: 6668–6673.[Abstract/Free Full Text]

55. Diep QN, Benkirane K, Schiffrin EL. Long-term effects of pioglitazone on cardiac hypertrophy, inflammation and fibrosis in stroke-prone spontaneously hypertensive rats. Hypertension. 2002; 40: 412.Abstract.

56. Wang C-H, Weisel RD, Liu PP, Fedak PWM, Verma S. Glitazones and heart failure: critical appraisal for the clinician. Circulation. 2003; 107: 1350–1354.[Free Full Text]




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