Published Online
on June 17, 2002

A more recent version of this article appeared on August 1, 2002

This Article Full Text (PDF) All Versions of this Article: 40/2/190    most recent 01.HYP.0000024267.71656.0Dv1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nishimoto, Y. Articles by Okumura, K. Search for Related Content PubMed Articles by Nishimoto, Y. Articles by Okumura, K. Related Collections Hypertension - basic studies

Submitted on January 10, 2002
Revised on January 29, 2002

Decrease in Renal Medullary Endothelial Nitric Oxide Synthase of Fructose-Fed, Salt-Sensitive Hypertensive Rats

Yasuhiro Nishimoto^*; Takahito Tomida; Hideo Matsui; Takayuki Ito; and Kenji Okumura

From the Internal Medicine II (Y.N., T.T., H.M., K.O.) and Health Sciences (T.I.), Nagoya University School of Medicine, Nagoya, Japan.

^* To whom correspondence should be addressed. E-mail: nisimoto{at}med.nagoya-u.ac.jp.

Abstract—We investigated the expression of endothelial NO synthase (eNOS) in the kidneys of fructose-fed insulin-resistant rats (FFR) with a low- or high-sodium diet. Male Sprague-Dawley rats were fed a control (C) or high-fructose (40% fructose; F) diet, with each coming in low-sodium (0.024% NaCl; LS-C or LS-F) or high-sodium (3% NaCl; HS-C or HS-F) varieties, for 2 weeks. Half of the FFR were orally administered pioglitazone (10 mg · kg^-1 · day^-1), an insulin-sensitizing agent (LS-FP or HS-FP). The systolic blood pressure was significantly higher in the HS-F rats than in the LS-F rats or the HS-C rats (HS-F rats, 129±4 mm Hg, versus LS-F rats, 115±3 mm Hg, P<0.05; or versus HS-C rats, 116±5 mm Hg, P<0.05), which indicated the salt dependence of hypertension in FFR. The protein expression of eNOS in the renal medulla of FFR was significantly lower than that in control rats during a high sodium load. The administration of pioglitazone prevented the hypertension (HS-F rats, 129±4 mm Hg, versus HS-FP rats, 113±3 mm Hg, P<0.05) and the reduction of medullary eNOS protein expression in HS-F rats. There was no significant difference in eNOS expression in the renal cortex or aorta between FFR and control rats, regardless of sodium load. These results suggest that the decrease in renal medullary NO production by eNOS during a high sodium load may play a role in fructose-fed, salt-sensitive hypertension.

Key words: insulin resistance • fructose • hypertension, sodium-dependent • nitric oxide synthase • kidney

This article has been cited by other articles:

				C.-C. Szeto and K.-M. Chow THIAZOLIDINEDIONES IN PERITONEAL DIALYSIS PATIENTS Perit. Dial. Int., May 1, 2009; 29(3): 248 - 251. [Full Text] [PDF]

				M. W. Brands, T. D. Bell, N. A. Rodriquez, P. Polavarapu, and D. Panteleyev Chronic glucose infusion causes sustained increases in tubular sodium reabsorption and renal blood flow in dogs Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2009; 296(2): R265 - R271. [Abstract] [Full Text] [PDF]

				W. E. Rodriguez, I. G. Joshua, J. C. Falcone, and S. C. Tyagi Pioglitazone prevents cardiac remodeling in high-fat, high-calorie-induced Type 2 diabetes mellitus Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H81 - H87. [Abstract] [Full Text] [PDF]

				P. Iglesias and J. J Diez Peroxisome proliferator-activated receptor gamma agonists in renal disease. Eur. J. Endocrinol., May 1, 2006; 154(5): 613 - 621. [Abstract] [Full Text] [PDF]

				D. Y. Huang, K. M. Boini, B. Friedrich, M. Metzger, L. Just, H. Osswald, P. Wulff, D. Kuhl, V. Vallon, and F. Lang Blunted hypertensive effect of combined fructose and high-salt diet in gene-targeted mice lacking functional serum- and glucocorticoid-inducible kinase SGK1 Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R935 - R944. [Abstract] [Full Text] [PDF]

				M. Herrera and J. L. Garvin Recent Advances in the Regulation of Nitric Oxide in the Kidney Hypertension, June 1, 2005; 45(6): 1062 - 1067. [Abstract] [Full Text] [PDF]

				J. Song, X. Hu, M. Shi, M. A. Knepper, and C. A. Ecelbarger Effects of dietary fat, NaCl, and fructose on renal sodium and water transporter abundances and systemic blood pressure Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1204 - F1212. [Abstract] [Full Text] [PDF]

				K. Sato, M. Kihara, T. Hashimoto, K. Matsushita, Y.-I. Koide, K. Tamura, N. Hirawa, Y. Toya, A. Fukamizu, and S. Umemura Alterations in Renal Endothelial Nitric Oxide Synthase Expression by Salt Diet in Angiotensin Type-1a Receptor Gene Knockout Mice J. Am. Soc. Nephrol., July 1, 2004; 15(7): 1756 - 1763. [Abstract] [Full Text] [PDF]

				Q. Ye, S. Chen, and D. G. Gardner Endothelin Inhibits NPR-A and Stimulates eNOS Gene Expression in Rat IMCD Cells Hypertension, March 1, 2003; 41(3): 675 - 681. [Abstract] [Full Text] [PDF]