on January 20, 2003
Published online before print January 20, 2003, doi: 10.1161/01.HYP.0000052945.84627.8F
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on September 19, 2002
From the Division of Nephrology and Hypertension and Center for Hypertension and Renal Disease Research, Georgetown University Medical Center, Washington, DC. * To whom correspondence should be addressed. E-mail: welchw{at}georgetown.edu.
Abstract--The spontaneously hypertensive rat (SHR) exhibits angiotensin II (Ang II)-dependent oxidative stress and reduced efficiency of renal oxygen usage (QO2) for tubular sodium transport (TNa). We tested the hypothesis that oxidative stress determines the reduced TNa:QO2 ratio in the clipped kidney of the early 2-kidney, 1-clip (2K,1C) Ang II-dependent model. One week after sham operation (Sham) or clip placement, 2K,1C rats received for 2 weeks either a vehicle, the superoxide dismutase mimetic tempol (Temp), or candesartan (Cand). Oxidative stress was assessed from excretion of 8-isoprostaglandin F2
Revised on October 21, 2002
Roles of Oxidative Stress and AT1 Receptors in Renal Hemodynamics and Oxygenation in the Postclipped 2K,1C Kidney
William J. Welch*;
(PGF2) and malondialdehyde (MDA) and renal oxygenation from pO2 in the renal cortex and from the ratio of calculated TNa and QO2 values. The mean arterial pressure (MAP) of Sham (113±6 mm Hg) was increased in 2K,1C vehicle-treated rats (148±4 mm Hg), but both Temp and Cand restored MAP to Sham levels. The excretions of 8-iso-PGF2 and MDA were higher in 2K,1C vehicle-treated rats compared with Sham and were normalized by Temp. The pO2 of Sham (42±2 mm Hg) was lower in 2K,1C vehicle-treated animals (28±2 mm Hg). This was restored to Sham values by Temp (36±3 mm Hg) but not by Cand (28±2 mm Hg). The TNa:QO2 of Sham (12.9±1.6) was reduced in 2K,1C vehicle-treated rats (9.7±2.8) and was restored to Sham values by Temp (13.7±2.5) but not by Cand (7.5±1.6). We conclude that the correction of oxidative stress in the 2K,1C model partially corrects renal cortical hypoxia and inefficient utilization of O2 for Na+ transport, independent of the fall in blood pressure.
Key words: hypertension, renovascular • Goldblatt hypertension • renal artery • nitric oxide
This article has been cited by other articles:
 |
|
 |
|
  S. Michaels, G. A. Eppel, S. L. Burke, G. A. Head, J. Armitage, J. F. Carroll, S. C. Malpas, and R. G. Evans Altered responsiveness of the kidney to activation of the renal nerves in fat-fed rabbits Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2009; 296(6): R1889 - R1896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Wilcox and A. Pearlman Chemistry and Antihypertensive Effects of Tempol and Other Nitroxides Pharmacol. Rev., December 1, 2008; 60(4): 418 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Evans, B. S. Gardiner, D. W. Smith, and P. M. O'Connor Intrarenal oxygenation: unique challenges and the biophysical basis of homeostasis Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1259 - F1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Cowley Jr Renal Medullary Oxidative Stress, Pressure-Natriuresis, and Hypertension Hypertension, November 1, 2008; 52(5): 777 - 786. [Full Text] [PDF]
|
|
|
|
|
|
L. M. Bivol, R. K. Berge, and B. M. Iversen Tetradecylthioacetic acid prevents the inflammatory response in two-kidney, one-clip hypertension Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R438 - R447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Palm, S. G. Connors, M. Mendonca, W. J. Welch, and C. S. Wilcox Angiotensin II Type 2 Receptors and Nitric Oxide Sustain Oxygenation in the Clipped Kidney of Early Goldblatt Hypertensive Rats Hypertension, February 1, 2008; 51(2): 345 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Welch, K. Patel, P. Modlinger, M. Mendonca, N. Kawada, K. Dennehy, S. Aslam, and C. S. Wilcox Roles of vasoconstrictor prostaglandins, COX-1 and -2, and AT1, AT2, and TP receptors in a rat model of early 2K,1C hypertension Am J Physiol Heart Circ Physiol, November 1, 2007; 293(5): H2644 - H2649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Meier, J. Rossert, P.-F. Plouin, and M. Burnier Atherosclerotic renovascular disease: beyond the renal artery stenosis Nephrol. Dial. Transplant., April 1, 2007; 22(4): 1002 - 1006. [Full Text] [PDF]
|
|
|
|
|
|
R. Olszanecki, R. Rezzani, S. Omura, D. E. Stec, L. Rodella, F. T. Botros, A. I. Goodman, G. Drummond, and N. G. Abraham Genetic suppression of HO-1 exacerbates renal damage: reversed by an increase in the antiapoptotic signaling pathway Am J Physiol Renal Physiol, January 1, 2007; 292(1): F148 - F157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-M. Lee, M.-S. Lin, C.-H. Tsai, and N.-C. Chang Effect of pravastatin on left ventricular mass in the two-kidney, one-clip hypertensive rats Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H2705 - H2713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kondo, H. Kiyomoto, T. Yamamoto, A. Miyatake, G.-P. Sun, M. Rahman, H. Hitomi, K. Moriwaki, T. Hara, S. Kimura, et al. Effects of Calcium Channel Blockade on Angiotensin II-Induced Peritubular Ischemia in Rats J. Pharmacol. Exp. Ther., March 1, 2006; 316(3): 1047 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wilcox Oxidative stress and nitric oxide deficiency in the kidney: a critical link to hypertension? Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R913 - R935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wesseling, D. A. Ishola Jr., J. A. Joles, H. A. Bluyssen, H. A. Koomans, and B. Braam Resistance to oxidative stress by chronic infusion of angiotensin II in mouse kidney is not mediated by the AT2 receptor Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1191 - F1200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sharma, A. Cook, M. Smith, C. Valancius, and E. W. Inscho TGF-{beta} impairs renal autoregulation via generation of ROS Am J Physiol Renal Physiol, May 1, 2005; 288(5): F1069 - F1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Welch, J. Blau, H. Xie, T. Chabrashvili, and C. S. Wilcox Angiotensin-induced defects in renal oxygenation: role of oxidative stress Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H22 - H28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Williams, J. S. Pollock, and D. M. Pollock Arterial Pressure Response to the Antioxidant Tempol and ETB Receptor Blockade in Rats on a High-Salt Diet Hypertension, November 1, 2004; 44(5): 770 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kawada, K. Dennehy, G. Solis, P. Modlinger, R. Hamel, J. T. Kawada, S. Aslam, T. Moriyama, E. Imai, W. J. Welch, et al. TP receptors regulate renal hemodynamics during angiotensin II slow pressor response Am J Physiol Renal Physiol, October 1, 2004; 287(4): F753 - F759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Chade, J. D. Krier, M. Rodriguez-Porcel, J. F. Breen, M. A. McKusick, A. Lerman, and L. O. Lerman Comparison of acute and chronic antioxidant interventions in experimental renovascular disease Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1079 - F1086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Chade, M. Rodriguez-Porcel, J. Herrmann, X. Zhu, J. P. Grande, C. Napoli, A. Lerman, and L. O. Lerman Antioxidant Intervention Blunts Renal Injury in Experimental Renovascular Disease J. Am. Soc. Nephrol., April 1, 2004; 15(4): 958 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Chade, M. Rodriguez-Porcel, J. Herrmann, J. D. Krier, X. Zhu, A. Lerman, and L. O. Lerman Beneficial Effects of Antioxidant Vitamins on the Stenotic Kidney Hypertension, October 1, 2003; 42(4): 605 - 612. [Abstract] [Full Text] [PDF]
|
|