on February 24, 2003
Published online before print February 24, 2003, doi: 10.1161/01.HYP.0000057573.32425.95
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on September 25, 2002
From INSERM U586, Faculté de Médecine, Laboratoire de Pharmacologie Médicale et Clinique, Toulouse Cedex, France. * To whom correspondence should be addressed. E-mail: Philippe.rouet{at}toulouse.inserm.fr.
Abstract--Obesity is associated with volumetric arterial hypertension and with early increase in heart rate and decreased heart rate variability. The consequences of obesity-related hypertension on heart gene regulation are poorly known and were investigated in a model of obesity-related hypertension induced by high fat diet in dogs. When compared with control animals (n=6), a 9-week high fat diet (n=6) provoked significant weight gain and increased blood pressure load and heart rate but failed to significantly change left ventricular mass assessed by echocardiography. Subtractive hybridization of dog heart cDNA libraries were used to generate sublibraries containing differentially expressed cDNAs that were in turn spotted onto membranes to create custom microarrays. Hybridizations of these microarrays with complex probes representing mRNAs expressed in right atria and left ventricles from obese hypertensive and control dogs were performed. Thirty-eight differentially expressed genes were identified; altered expression was confirmed by Northern blot analysis in 15. In addition, real-time quantitative polymerase chain reaction confirmed differential expression for 80% of the randomly chosen tested genes. Once identified, transcripts were categorized into groups involved in metabolism, cell signaling, ionic regulation, cell proliferation, protein synthesis, and tissue remodeling. In addition, we found a set of 11 cDNAs encoding proteins with unknown functions. This study clearly shows that obesity-related hypertension, lasting for only 9 weeks, causes marked changes in gene expression in right atrium as well as the left ventricle that may contribute to early functional changes in heart function and to long-term structural changes such as left ventricular hypertrophy and remodeling.
Revised on October 16, 2002
Cardiac Transcriptome Analysis in Obesity-Related Hypertension
Pierre Philip-Couderc;
Key words: obesity • hypertension, arterial • heart rate • heart • dogs
This article has been cited by other articles:
 |
|
 |
|
  S. Kenchaiah, H. D. Sesso, and J. M. Gaziano Body Mass Index and Vigorous Physical Activity and the Risk of Heart Failure Among Men Circulation, January 6, 2009; 119(1): 44 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lamant, F. Smih, R. Harmancey, P. Philip-Couderc, A. Pathak, J. Roncalli, M. Galinier, X. Collet, P. Massabuau, J.-M. Senard, et al. ApoO, a Novel Apolipoprotein, Is an Original Glycoprotein Up-regulated by Diabetes in Human Heart J. Biol. Chem., November 24, 2006; 281(47): 36289 - 36302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hlaing, P. Spitz, K. Padmanabhan, B. Cabezas, C. S. Barker, and H. S. Bernstein E2F-1 Regulates the Expression of a Subset of Target Genes during Skeletal Myoblast Hypertrophy J. Biol. Chem., October 15, 2004; 279(42): 43625 - 43633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Philip-Couderc, F. Smih, J. E. Hall, A. Pathak, J. Roncalli, R. Harmancey, P. Massabuau, M. Galinier, P. Verwaerde, J.-M. Senard, et al. Kinetic analysis of cardiac transcriptome regulation during chronic high-fat diet in dogs Physiol Genomics, September 16, 2004; 19(1): 32 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R S Vasan Cardiac function and obesity Heart, October 1, 2003; 89(10): 1127 - 1129. [Full Text] [PDF]
|
|