Published Online
on August 4, 2003

A more recent version of this article appeared on October 1, 2003

This Article Full Text (PDF) All Versions of this Article: 42/4/787    most recent 01.HYP.0000085334.34963.4Ev1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zeng, C. Articles by Jose, P. A. Search for Related Content PubMed PubMed Citation Articles by Zeng, C. Articles by Jose, P. A. Related Collections Hypertension - basic studies

Submitted on May 12, 2003
Revised on May 27, 2003

Perturbation of D₁ Dopamine and AT₁ Receptor Interaction in Spontaneously Hypertensive Rats

Chunyu Zeng^*; Yingjin Luo; Laureano D. Asico; Ulrich Hopfer; Gilbert M. Eisner; Robin A. Felder; and Pedro A. Jose

From the Departments of Pediatrics (C.Z., Y.L., L.D.A., G.M.E., P.A.J.), Physiology and Biophysics (P.A.J.), and Internal Medicine (G.M.E.), Georgetown University Medical Center, Washington, DC; the Department of Physiology (U.H.), Case Western Reserve School of Medicine, Cleveland, Ohio; the Department of Pathology (R.A.F.), Virginia University for the Health Sciences, Charlottesville, Va; and the Department of Cardiology (C.Z.), Daping Hospital, Third Military Medical University, Chongqing, Peoples Republic of China.

^* To whom correspondence should be addressed. E-mail: cyzeng1{at}hotmail.com.

Abstract--The dopaminergic and renin-angiotensin systems interact to regulate blood pressure. Because this interaction may be perturbed in genetic hypertension, we studied D₁ dopamine and AT₁ angiotensin receptors in immortalized renal proximal tubule (RPT) and A10 aortic vascular smooth muscle cells. In normotensive Wistar-Kyoto (WKY) rats, the D₁-like agonist fenoldopam increased D₁ receptors but decreased AT₁ receptors. These effects were blocked by the D₁-like antagonist SCH 23390 (10^-7 mol/L per 24 hours). In spontaneously hypertensive rat (SHR) RPT cells, fenoldopam also decreased AT₁ receptors but no longer stimulated D₁ receptor expression. Basal levels of AT₁/D₁ receptor coimmunoprecipitation were greater in WKY RPT cells (29±2 density units, DU) than in SHR RPT cells (21±2 DU, n=7 per group, P<0.05). The coimmunoprecipitation of D₁ and AT₁ receptors was increased by fenoldopam (10^-7 mol/L per 24 hours) in WKY RPT cells but decreased in SHR RPT cells. The effects of fenoldopam in RPT cells from WKY rats were similar in aortic vascular smooth muscle cells from normotensive BD IX rats, that is, fenoldopam decreased AT₁ receptors and increased D₁ receptors. Our studies show differential regulation of the expression of D₁ and AT₁ receptors in RPT cells from WKY and SHR. This regulation and D₁/AT₁ receptor interaction are different in RPT cells of WKY and SHR. An altered interaction of D₁ and AT₁ receptors may play a role in the impaired sodium excretion and enhanced vasoconstriction in hypertension.

Key words: hypertension, essential • receptors, angiotensin II • dopamine • kidney • muscle, smooth, vascular

This article has been cited by other articles:

				J. J. Gildea, J. A. Israel, A. K. Johnson, J. Zhang, P. A. Jose, and R. A. Felder Caveolin-1 and Dopamine-Mediated Internalization of NaKATPase in Human Renal Proximal Tubule Cells Hypertension, November 1, 2009; 54(5): 1070 - 1076. [Abstract] [Full Text] [PDF]

				V. A. M. Villar, J. E. Jones, I. Armando, C. Palmes-Saloma, P. Yu, A. M. Pascua, L. Keever, F. B. Arnaldo, Z. Wang, Y. Luo, et al. G Protein-coupled Receptor Kinase 4 (GRK4) Regulates the Phosphorylation and Function of the Dopamine D3 Receptor J. Biol. Chem., August 7, 2009; 284(32): 21425 - 21434. [Abstract] [Full Text] [PDF]

				J. L. Hansen, J. T. Hansen, T. Speerschneider, C. Lyngso, N. Erikstrup, E. S. Burstein, D. M. Weiner, T. Walther, N. Makita, T. Iiri, et al. Lack of Evidence for AT1R/B2R Heterodimerization in COS-7, HEK293, and NIH3T3 Cells: HOW COMMON IS THE AT1R/B2R HETERODIMER? J. Biol. Chem., January 16, 2009; 284(3): 1831 - 1839. [Abstract] [Full Text] [PDF]

				M. Yamada, M. Kushibiki, T. Osanai, H. Tomita, and K. Okumura Vasoconstrictor effect of aldosterone via angiotensin II type 1 (AT1) receptor: possible role of AT1 receptor dimerization Cardiovasc Res, July 1, 2008; 79(1): 169 - 178. [Abstract] [Full Text] [PDF]

				C. Zeng, V. A. M. Villar, G. M. Eisner, S. M. Williams, R. A. Felder, and P. A. Jose G Protein-Coupled Receptor Kinase 4: Role in Blood Pressure Regulation Hypertension, June 1, 2008; 51(6): 1449 - 1455. [Full Text] [PDF]

				C. Zeng, I. Armando, Y. Luo, G. M. Eisner, R. A. Felder, and P. A. Jose Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H551 - H569. [Abstract] [Full Text] [PDF]

				J. J. Gildea, X. Wang, P. A. Jose, and R. A. Felder Differential D1 and D5 Receptor Regulation and Degradation of the Angiotensin Type 1 Receptor Hypertension, February 1, 2008; 51(2): 360 - 366. [Abstract] [Full Text] [PDF]

				M. Mogi, M. Iwai, and M. Horiuchi Emerging Concepts of Regulation of Angiotensin II Receptors: New Players and Targets for Traditional Receptors Arterioscler Thromb Vasc Biol, December 1, 2007; 27(12): 2532 - 2539. [Abstract] [Full Text] [PDF]

				J. L. Cook, S. J. Mills, R. T. Naquin, J. Alam, and R. N. Re Cleavage of the angiotensin II type 1 receptor and nuclear accumulation of the cytoplasmic carboxy-terminal fragment Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1313 - C1322. [Abstract] [Full Text] [PDF]

				P. K. Mehta and K. K. Griendling Angiotensin II cell signaling: physiological and pathological effects in the cardiovascular system Am J Physiol Cell Physiol, January 1, 2007; 292(1): C82 - C97. [Abstract] [Full Text] [PDF]

				H. Sanada, J. Yatabe, S. Midorikawa, T. Katoh, S. Hashimoto, T. Watanabe, J. Xu, Y. Luo, X. Wang, C. Zeng, et al. Amelioration of Genetic Hypertension by Suppression of Renal G Protein-Coupled Receptor Kinase Type 4 Expression Hypertension, June 1, 2006; 47(6): 1131 - 1139. [Abstract] [Full Text] [PDF]

				C. Zeng, Z. Wang, H. Li, P. Yu, S. Zheng, L. Wu, L. D. Asico, U. Hopfer, G. M. Eisner, R. A. Felder, et al. D3 Dopamine Receptor Directly Interacts With D1 Dopamine Receptor in Immortalized Renal Proximal Tubule Cells Hypertension, March 1, 2006; 47(3): 573 - 579. [Abstract] [Full Text] [PDF]

				M. J. Bek, X. Wang, L. D. Asico, J. E. Jones, S. Zheng, X. Li, G. M. Eisner, D. K. Grandy, R. M. Carey, P. Soares-da-Silva, et al. Angiotensin-II Type 1 Receptor-Mediated Hypertension in D4 Dopamine Receptor-Deficient Mice Hypertension, February 1, 2006; 47(2): 288 - 295. [Abstract] [Full Text] [PDF]

				C. Zeng, U. Hopfer, L. D. Asico, G. M. Eisner, R. A. Felder, and P. A. Jose Altered AT1 Receptor Regulation of ETB Receptors in Renal Proximal Tubule Cells of Spontaneously Hypertensive Rats Hypertension, October 1, 2005; 46(4): 926 - 931. [Abstract] [Full Text] [PDF]

				C. Zeng, Z. Wang, U. Hopfer, L. D. Asico, G. M. Eisner, R. A. Felder, and P. A. Jose Rat Strain Effects of AT1 Receptor Activation on D1 Dopamine Receptors in Immortalized Renal Proximal Tubule Cells Hypertension, October 1, 2005; 46(4): 799 - 805. [Abstract] [Full Text] [PDF]

				Y.-H. Feng, Y. Ding, S. Ren, L. Zhou, C. Xu, and S. S. Karnik Unconventional Homologous Internalization of the Angiotensin II Type-1 Receptor Induced by G-Protein-Independent Signals Hypertension, August 1, 2005; 46(2): 419 - 425. [Abstract] [Full Text] [PDF]

				M.-Z. Zhang, B. Yao, J. A. McKanna, and R. C. Harris Cross talk between the intrarenal dopaminergic and cyclooxygenase-2 systems Am J Physiol Renal Physiol, April 1, 2005; 288(4): F840 - F845. [Abstract] [Full Text] [PDF]

				C. Zeng, Z. Yang, Z. Wang, J. Jones, X. Wang, J. Altea, A. J. Mangrum, U. Hopfer, D. R. Sibley, G. M. Eisner, et al. Interaction of Angiotensin II Type 1 and D5 Dopamine Receptors in Renal Proximal Tubule Cells Hypertension, April 1, 2005; 45(4): 804 - 810. [Abstract] [Full Text] [PDF]

				Z. Yang, L. D. Asico, P. Yu, Z. Wang, J. E. Jones, R.-k. Bai, D. R. Sibley, R. A. Felder, and P. A. Jose D5 dopamine receptor regulation of phospholipase D Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H55 - H61. [Abstract] [Full Text] [PDF]

				C. Zeng, H. Sanada, H. Watanabe, G. M. Eisner, R. A. Felder, and P. A. Jose Functional genomics of the dopaminergic system in hypertension Physiol Genomics, November 17, 2004; 19(3): 233 - 246. [Abstract] [Full Text] [PDF]

				J. M. Stern, J. Chen, R. B. Silver, D. P. Poppas, E. D. Vaughan Jr., and D. Felsen Effect of UUO on D1aR expression reveals a link among dopamine, transforming growth factor-{beta}, and nitric oxide Am J Physiol Renal Physiol, March 1, 2004; 286(3): F509 - F515. [Abstract] [Full Text]

				C. Zeng, D. Wang, L. D. Asico, W. J. Welch, C. S. Wilcox, U. Hopfer, G. M. Eisner, R. A. Felder, and P. A. Jose Aberrant D1 and D3 Dopamine Receptor Transregulation in Hypertension Hypertension, March 1, 2004; 43(3): 654 - 660. [Abstract] [Full Text] [PDF]