Published Online
on December 29, 2003

A more recent version of this article appeared on February 1, 2004

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Submitted on September 30, 2003
Revised on October 27, 2003

Nitric Oxide Synthesis Inhibition Promotes Renal Production of Carbon Monoxide

Francisca Rodriguez^*; Brian D. Lamon; Weiying Gong; Rowena Kemp; and Alberto Nasjletti

From the Department of Pharmacology, New York Medical College, Valhalla.

^* To whom correspondence should be addressed. E-mail: francisca_rodrigues{at}nymc.edu.

Abstract--We tested the hypothesis that the status of NO synthesis influences the renal heme-heme oxygenase system. Studies were conducted in untreated rats and rats treated with the NO synthesis inhibitor N^G-nitro-L-arginine methyl ester for 2 days. Treated and untreated rats were contrasted in terms of renal expression of heme oxygenase-1 and -2, renal carbon monoxide (CO)-generating activity, and urinary CO concentration and excretion rate. Heme oxygenase-1 and -2 proteins were similarly expressed in the kidneys of untreated and treated rats. In contrast, the NADPH-dependent component of the CO-generating activity of renal homogenates incubated with heme (a measure of heme oxygenase activity) was higher (P<0.05) in kidneys from rats treated with the NO synthesis inhibitor relative to corresponding data in untreated rats (1015±95 versus 379±111 pmol CO/mg per hour). Similarly, relative to corresponding data in untreated rats, rats treated with the NO synthesis inhibitor displayed increased (P<0.05) urinary CO concentration (920±174 versus 2286±472 pmol/mL) and urinary CO excretion (4.7±0.4 versus 14.3±2.7 pmol/min). This study demonstrates that NO synthesis inhibition upregulates the urinary concentration and excretion rate of CO, and the HO-dependent generation of CO by renal homogenates, without affecting the expression of renal heme oxygenase isoforms. Our findings imply that endogenous NO is an inhibitory regulator of renal CO generation by HO.

Key words: kidney • carbon monoxide • nitric oxide • heme oxygenase

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