on February 16, 2004
Published online before print February 16, 2004, doi: 10.1161/01.HYP.0000120123.44945.47
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Submitted on November 3, 2003
From Medical College of Wisconsin (K.M.H., A.K.F., A.J.D.-V., E.A.d.S., R.J.R.), Department of Physiology, Milwaukee; Laboratory of Kidney and Electrolyte Metabolism (M.A.K.), National Heart, Lung and Blood Institute, Bethesda, Md. * To whom correspondence should be addressed. E-mail: rroman{at}mcw.edu.
Abstract--This study compared the expression of enzymes and transport and channel proteins involved in the regulation of sodium reabsorption in the kidney of Dahl salt-sensitive (DS) and salt-resistant Brown-Norway (BN) and consomic rats (SS.BN13), in which chromosome 13 from the BN rat has been introgressed into the DS genetic background. The expression of the Na+/K+/2Cl- (BSC-1) cotransporter, Na+/H+ exchanger (NHE3), and Na+-K+-ATPase proteins were similar in the renal cortex of DS, BN, and SS.BN13 rats fed either a low-salt (0.1% NaCl) or a high-salt (8% NaCl) diet. The expression of the BSC-1 and the renal outer medullary K+ channel (ROMK) were higher, whereas the expression of the cytochrome P4504A proteins responsible for the formation of 20-hydroxyeicosatetraenoic (20-HETE) was lower in the outer medulla of the kidney of DS than in BN or SS.BN13 rats fed either a low-salt or a high-salt diet. In addition, the renal formation and excretion of 20-HETE was lower in DS than in BN and SS.BN13 rats. These results suggest that overexpression of ROMK and BSC-1 in the thick ascending limb combined with a deficiency in renal formation of 20-HETE may predispose Dahl S rats fed a high-salt diet to Na+ retention and hypertension.
Revised on November 24, 2003
Elevated BSC-1 and ROMK Expression in Dahl Salt-Sensitive Rat Kidneys
Kimberly M. Hoagland;
Key words: rats, Dahl • Na+, K+-transporting ATPase • sodium • hypertension
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