Published Online
on July 5, 2005

A more recent version of this article appeared on August 1, 2005

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Submitted on February 8, 2005
Revised on February 26, 2005

Angiotensin II-Mediated Phenotypic Cardiomyocyte Remodeling Leads to Age-Dependent Cardiac Dysfunction and Failure

Andrea A. Domenighetti; Qing Wang; Marcel Egger; Stephen M. Richards; Thierry Pedrazzini; and Lea M.D. Delbridge^*

From the Department of Physiology (A.A.D., L.M.D.D.), University of Melbourne, Australia; the Department of Medicine (Q.W., T.P.), University of Lausanne Medical School, Switzerland; the Department of Physiology (M.E.), University of Bern, Switzerland; and the Division of Biochemistry (S.M.R.), University of Tasmania, Australia.

^* To whom correspondence should be addressed. E-mail: lmd{at}unimelb.edu.au.

Abstract--Chronic elevation of plasma angiotensin II (Ang II) is detrimental to the heart. In addition to its hemodynamic effects, Ang II exerts cardiotrophic actions that contribute to cardiomyocyte remodeling. However, it remains to be clarified whether these direct actions of Ang II are sufficient to cause contractile dysfunction and heart failure in the absence of altered hemodynamic conditions. In this study, we used TG1306/1R (TG) mice that develop Ang II-mediated cardiac hypertrophy in absence of elevated blood pressure to investigate the phenotypic changes in cardiomyocytes during the adaptive response to chronic cardiac-specific endogenous Ang II stimulation. A 94-week longitudinal study demonstrated that TG mice develop dilated cardiomyopathy with aging and exhibit a significant increase in mortality compared with wild-type (WT) mice. Cardiac hypertrophy in TG mice is associated with cardiomyocyte hypertrophy (15 to 20 weeks: length +20%; 35 to 40 weeks: length +10%, width +15%) but not collagen deposition. In vivo analysis of cardiac function revealed age-dependent systolic and diastolic dysfunction in TG mice (45% reduction in dP/dt_max and dP/dt_min at 50 to 60 weeks of age compared with WT). Analysis of isolated cardiomyocyte isotonic shortening showed impaired contractility in TG cardiomyocytes (30% to 40% decrease in rates of shortening and lengthening). In TG hearts, chronic Ang II exposure induced downregulation of the sarcoplasmic reticulum calcium pump (SERCA2) and diminution of Ca²⁺ transients, indicative of an underlying disturbance in calcium homeostasis. In conclusion, chronic Ang II myocardial stimulation without hemodynamic overload is sufficient to produce cardiomyocyte and cardiac dysfunction culminating in heart failure.

Key words: aging • cardiac function • heart failure • hypertrophy • myocytes • renin-angiotensin system

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