Hypertension, Vol 14, 413-420, Copyright © 1989 by American Heart Association
AA Geisterfer and GK Owens
Recently we reported that the contractile agonist angiotensin II induces
hypertrophy, not hyperplasia, in cultured rat aortic smooth muscle cells
(Geisterfer AAT, Peach MJ, Owens GK: Angiotensin II induces hypertrophy,
not hyperplasia, of cultured rat aortic smooth muscle cells. Circ Res
1988;62:749-756). We have further explored the hypothesis that contractile
agonists are important regulators of smooth muscle cell growth by examining
the effects of another contractile agonist, arginine vasopressin, on growth
of cultured rat aortic smooth muscle cells. Autoradiographic analysis as
well as cell number determinations showed that arginine vasopressin (1
microM) did not stimulate proliferation in cells made quiescent in a
defined serum-free media nor did it augment proliferation in 0.4% fetal
bovine serum. However, flow cytometric analysis of cellular protein content
demonstrated that arginine vasopressin (1 microM) did induce cellular
hypertrophy in quiescent cultures after 4 days of treatment, increasing
smooth muscle cell protein content by 35% as compared with vehicle- treated
controls. The increase in protein content showed a concentration
dependence. Cellular hypertrophy was accompanied by an increase in
[35S]methionine incorporation, which was elevated 45% by 24 hours. Both the
increase in [35S]methionine incorporation and the increase in protein
content could be prevented by the specific arginine vasopressin receptor
antagonist. [1-beta-mercapto-beta,beta- cyclopentamethylene propionic
acid), 2-(O-methyl)tyrosine] arginine vasopressin. An increase in
[35S]methionine incorporation was observed between 12 and 24 hours after
treatment of quiescent smooth muscle cells for only 5 minutes with arginine
vasopressin (1 microM). Arginine vasopressin-induced increases in
[35S]methionine incorporation was increased within 6 hours after treatment.
These studies show that arginine vasopressin, like angiotensin II, induces
hypertrophy but not hyperplasia of cultured rat aortic smooth muscle cells.
ARTICLES
Arginine vasopressin-induced hypertrophy of cultured rat aortic smooth muscle cells
Department of Physiology University of Virginia, School of Medicine, Charlottesville 22908.
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