This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cachofeiro, V. Articles by Nasjletti, A. Search for Related Content PubMed PubMed Citation Articles by Cachofeiro, V. Articles by Nasjletti, A.

Hypertension, Vol 18, 683-688, Copyright © 1991 by American Heart Association

ARTICLES

Increased vascular responsiveness to bradykinin in kidneys of spontaneously hypertensive rats. Effect of N omega-nitro-L-arginine

V Cachofeiro and A Nasjletti
Department of Pharmacology, New York Medical College, Valhalla 10595.

We investigated the role of nitric oxide (NO)-dependent and NO- independent mechanisms in mediation of renal vasodilatory responses to bradykinin in spontaneously hypertensive rats (SHR), rats with angiotensin II-induced hypertension (200 ng/min i.p. for 6 days) and the corresponding normotensive control Wistar-Kyoto (WKY) rats and sham- infused rats. To this end, we contrasted the effects of arterial injections of bradykinin and other vasodilators, acetylcholine and sodium nitroprusside, on perfusion pressure and output of cyclic GMP in isolated kidneys perfused with Krebs bicarbonate buffer containing phenylephrine, both with and without N omega-nitro-L-arginine (L-NOARG) (50 microM), an inhibitor of NO synthetase. In kidneys perfused without L-NOARG, all agonists increased the output of cyclic GMP and reduced perfusion pressure, indicative of vasodilation. In kidneys perfused with L-NOARG, vasodilatory responses to bradykinin and acetylcholine were attenuated, and associated effects on output of cyclic GMP were abolished, suggesting dependency on NO synthesis. Irrespective of whether kidneys were perfused with or without L-NOARG, kidneys of SHR were more responsive than kidneys of WKY rats with regard to bradykinin- induced vasodilation. In contrast, vasodilatory responsiveness to bradykinin was nearly equal in perfused kidneys of rats with angiotensin II-induced hypertension and in normotensive controls. Also, vasodilatory responsiveness to acetylcholine and sodium nitroprusside was similar in kidneys of normotensive and hypertensive rats. These data suggest that the renal vasculature of SHR is uniquely and selectively hyperresponsive to bradykinin, with regard to both the NO- dependent and NO-independent vasodilatory actions.

This article has been cited by other articles:

				S. I. Pomposiello, J. Quilley, M. A. Carroll, J. R. Falck, and J. C. McGiff 5,6-Epoxyeicosatrienoic Acid Mediates the Enhanced Renal Vasodilation to Arachidonic Acid in the SHR Hypertension, October 1, 2003; 42(4): 548 - 554. [Abstract] [Full Text] [PDF]

				Y.-J. Chen, H. Jiang, and J. Quilley The Nitric Oxide- and Prostaglandin-Independent Component of the Renal Vasodilator Effect of Thimerosal Is Mediated by Epoxyeicosatrienoic Acids J. Pharmacol. Exp. Ther., March 1, 2003; 304(3): 1292 - 1298. [Abstract] [Full Text] [PDF]

				S. I. Pomposiello, M. A. Carroll, J. R. Falck, and J. C. McGiff Epoxyeicosatrienoic Acid-Mediated Renal Vasodilation to Arachidonic Acid Is Enhanced in SHR Hypertension, March 1, 2001; 37(3): 887 - 893. [Abstract] [Full Text] [PDF]

				B. M. Marcic and E. G. Erdös Protein Kinase C and Phosphatase Inhibitors Block the Ability of Angiotensin I-Converting Enzyme Inhibitors to Resensitize the Receptor to Bradykinin without Altering the Primary Effects of Bradykinin J. Pharmacol. Exp. Ther., August 1, 2000; 294(2): 605 - 612. [Abstract] [Full Text]

				Y. Nishikawa, D. W. Stepp, and W. M. Chilian Nitric oxide exerts feedback inhibition on EDHF-induced coronary arteriolar dilation in vivo Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H459 - H465. [Abstract] [Full Text] [PDF]

				Y. Nishikawa, D. W. Stepp, and W. M. Chilian In vivo location and mechanism of EDHF-mediated vasodilation in canine coronary microcirculation Am J Physiol Heart Circ Physiol, September 1, 1999; 277(3): H1252 - H1259. [Abstract] [Full Text] [PDF]

				W. J. Welch, A. Tojo, J.-U. Lee, D. G. Kang, C. G. Schnackenberg, and C. S. Wilcox Nitric oxide synthase in the JGA of the SHR: expression and role in tubuloglomerular feedback Am J Physiol Renal Physiol, July 1, 1999; 277(1): F130 - F138. [Abstract] [Full Text] [PDF]

				H. P. Brunner-La Rocca, G. Vaddadi, and M. D. Esler Recent insight into therapy of congestive heart failure: focus on ACE inhibition and angiotensin-II antagonism J. Am. Coll. Cardiol., April 1, 1999; 33(5): 1163 - 1173. [Abstract] [Full Text] [PDF]

				P. Mieyal, D. Fulton, J.C. McGiff, and J. Quilley NO-Independent Vasodilation to Acetylcholine in the Rat Isolated Kidney Utilizes a Charybdotoxin-Sensitive, Intermediate-Conductance Ca++-Activated K+ Channel J. Pharmacol. Exp. Ther., May 1, 1998; 285(2): 659 - 664. [Abstract] [Full Text]

				R. Maeso, J. Navarro-Cid, R. Munoz-Garcia, E. Rodrigo, L. M. Ruilope, V. Lahera, and V. Cachofeiro Losartan Reduces Phenylephrine Constrictor Response in Aortic Rings From Spontaneously Hypertensive Rats: Role of Nitric Oxide and Angiotensin II Type 2 Receptors Hypertension, December 1, 1996; 28(6): 967 - 972. [Abstract] [Full Text]

				Y. Erlich and T. Rosenthal Contribution of Nitric Oxide to the Beneficial Effects of Enalapril in the Fructose-Induced Hyperinsulinemic Rat Hypertension, November 1, 1996; 28(5): 754 - 757. [Abstract] [Full Text]

				D. Rees, D. Ben-Ishay, and S. Moncada Nitric Oxide and the Regulation of Blood Pressure in the Hypertension-Prone and Hypertension-Resistant Sabra Rat Hypertension, September 1, 1996; 28(3): 367 - 371. [Abstract] [Full Text]

				H. S. Margolius Kallikreins and Kinins : Some Unanswered Questions About System Characteristics and Roles in Human Disease Hypertension, August 1, 1995; 26(2): 221 - 229. [Abstract] [Full Text]

				V. Cachofeiro, R. Maeso, E. Rodrigo, J. Navarro, L. M. Ruilope, and V. Lahera Nitric Oxide and Prostaglandins in the Prolonged Effects of Losartan and Ramipril in Hypertension Hypertension, August 1, 1995; 26(2): 236 - 243. [Abstract] [Full Text]

				K. Hayashi, H. Suzuki, and T. Saruta Nitric Oxide Modulates but Does Not Impair Myogenic Vasoconstriction of the Afferent Arteriole in Spontaneously Hypertensive Rats : Studies in the Isolated Perfused Hydronephrotic Kidney Hypertension, June 1, 1995; 25(6): 1212 - 1219. [Abstract] [Full Text]

				H. Nakamoto, C. M. Ferrario, S. B. Fuller, D. L. Robaczewski, E. Winicov, and R. H. Dean Angiotensin-(1-7) and Nitric Oxide Interaction in Renovascular Hypertension Hypertension, April 1, 1995; 25(4): 796 - 802. [Abstract] [Full Text]

				M. Kelm, M. Feelisch, T. Krebber, A. Deußen, W. Motz, and B. E. Strauer Role of Nitric Oxide in the Regulation of Coronary Vascular Tone in Hearts From Hypertensive Rats : Maintenance of Nitric Oxide–Forming Capacity and Increased Basal Production of Nitric Oxide Hypertension, February 1, 1995; 25(2): 186 - 193. [Abstract] [Full Text]