This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Simonet, L. Articles by Kurtz, T. W. Search for Related Content PubMed PubMed Citation Articles by Simonet, L. Articles by Kurtz, T. W. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information High Blood Pressure Hazardous Substances DB SODIUM CHLORIDE

Hypertension, Vol 18, 689-693, Copyright © 1991 by American Heart Association

ARTICLES

Sequence analysis of the alpha 1 Na+,K(+)-ATPase gene in the Dahl salt- sensitive rat

L Simonet, E St. Lezin and TW Kurtz
Department of Laboratory Medicine, University of California, San Francisco 94143-0134.

In the inbred Dahl salt-sensitive rat (SS/Jr strain), it has been proposed that a T for A transversion in the DNA sequence encoding amino acid 276 in the alpha 1 subunit isoform of Na+,K(+)-ATPase may impair ion transport and contribute to the pathogenesis of hypertension. This hypothesis is of major scientific interest because it represents the first attempt to explain the pathogenesis of salt-sensitive hypertension on the basis of a specifically defined mutation at the DNA level. We devised a polymerase chain reaction technique to screen the genomic DNA of multiple SS/Jr rats for the T for A transversion reported in the complementary DNA (cDNA) encoding the alpha 1 subunit of Na+,K(+)-ATPase. When eight Dahl SS/Jr rats from Harlan Sprague Dawley Inc. were tested with the polymerase chain reaction technique, we found no evidence of this mutation in the Na+,K(+)-ATPase gene. Direct sequence analysis of the gene in three SS/Jr rats also did not show the T for A transversion. These results 1) strongly suggest that commercially available Dahl SS/Jr rats do not carry a T for A transversion in the genomic DNA sequence encoding amino acid 276 in the alpha 1 subunit isoform of Na+,K(+)-ATPase and 2) raise the possibility that the previous finding of a mutation in the cDNA of the SS/Jr rat may have been due to a reverse transcriptase error during cDNA synthesis.

This article has been cited by other articles:

				M. Mokry and E. Cuppen The Atp1a1 Gene From Inbred Dahl Salt Sensitive Rats Does Not Contain the A1079T Missense Transversion Hypertension, April 1, 2008; 51(4): 922 - 927. [Abstract] [Full Text] [PDF]

				J. M. Saavedra The Challenge of Genetic Studies in Hypertension Circ. Res., May 25, 2007; 100(10): 1389 - 1393. [Full Text] [PDF]

				R. Barnard, G. Kelly, S. O. Manzetti, and E. L. Harris Neither the New Zealand Genetically Hypertensive Strain nor Dahl Salt-Sensitive Strain Has an A1079T Transversion in the {alpha}1 Isoform of the Na+,K+-ATPase Gene Hypertension, October 1, 2001; 38(4): 786 - 792. [Abstract] [Full Text] [PDF]

				J. ZICHA, C. D. NEGRIN, Z. DOBESOVA, F. CARR, M. VOKURKOVA, M. W. MCBRIDE, J. KUNES, and A. F. DOMINICZAK Altered Na+-K+ pump activity and plasma lipids in salt-hypertensive Dahl rats: relationship to Atp1a1 gene Physiol Genomics, July 17, 2001; 6(2): 99 - 104. [Abstract] [Full Text] [PDF]

				J. P. Rapp Genetic Analysis of Inherited Hypertension in the Rat Physiol Rev, January 1, 2000; 80(1): 135 - 172. [Abstract] [Full Text] [PDF]

				S. N. Orlov, N. C. Adragna, V. A. Adarichev, and P. Hamet Genetic and biochemical determinants of abnormal monovalent ion transport in primary hypertension Am J Physiol Cell Physiol, March 1, 1999; 276(3): C511 - C536. [Abstract] [Full Text] [PDF]

				D. E. Orosz and U. Hopfer Pathophysiological Consequences of Changes in the Coupling Ratio of Na,K-ATPase for Renal Sodium Reabsorption and Its Implications for Hypertension Hypertension, February 1, 1996; 27(2): 219 - 227. [Abstract] [Full Text]

				S. N. ORLOV, J. DUTIL, P. HAMET, and A. Y. DENG Replacement of {alpha}1-Na-K-ATPase of Dahl rats by Milan rats lowers blood pressure but does not affect its activity Physiol Genomics, December 21, 2001; 7(2): 171 - 177. [Abstract] [Full Text] [PDF]