This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Dominiczak, A. F. Articles by Bohr, D. F. Search for Related Content PubMed PubMed Citation Articles by Dominiczak, A. F. Articles by Bohr, D. F. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL LECITHIN Medline Plus Health Information High Blood Pressure

Hypertension, Vol 18, 748-757, Copyright © 1991 by American Heart Association

ARTICLES

Lipid bilayer in genetic hypertension

AF Dominiczak, DF Lazar, AK Das and DF Bohr
Department of Physiology, University of Michigan, Ann Arbor.

Membrane microviscosity, phospholipid composition, and turnover were measured in cultured vascular smooth muscle cells isolated from mesenteric arteries of stroke-prone spontaneously hypertensive and age- matched, normotensive Wistar-Kyoto rats. Membrane microviscosity, measured with fluorescence polarization, revealed greater microviscosity (lower fluidity) of the membranes isolated from smooth muscle cells from hypertensive as compared with those isolated from normotensive rats (p less than 0.01). Preincubation of membranes from hypertensive rats with 5 mM calcium reduced membrane microviscosity in "core" and in "surface" regions of the bilayer toward values observed in Wistar-Kyoto rats. Phospholipid composition did not differ between intact aortas and cultured mesenteric cells or between those tissues obtained from normotensive and from hypertensive rats. The total lipid- associated radioactivity was significantly lower in cells from stroke- prone spontaneously hypertensive rats than in those from Wistar-Kyoto controls (p less than 0.01). Phosphatidylcholine incorporated 70% and phosphatidylinositol 16% of total lipid-associated radioactivity, with no difference between cells from hypertensive and normotensive animals. Turnover of phosphatidylethanolamine was greater in cells from Wistar- Kyoto rats (p = 0.02), whereas turnover of phosphatidylserine was greater in cells from stroke-prone spontaneously hypertensive rats (p = 0.04). The greater microviscosity of the lipid bilayer in hypertension is a generalized defect of the matrix in which the transport proteins function. We hypothesize that this defect is responsible for the multiple abnormalities of membrane transport systems that have been described in genetic hypertension.

This article has been cited by other articles:

				R. Alemany, S. Teres, C. Baamonde, M. Benet, O. Vogler, and P. V. Escriba 2-Hydroxyoleic Acid: A New Hypotensive Molecule Hypertension, February 1, 2004; 43(2): 249 - 254. [Abstract] [Full Text] [PDF]

				P. V. Escriba, J. M. Sanchez-Dominguez, R. Alemany, J. S. Perona, and V. Ruiz-Gutierrez Alteration of Lipids, G Proteins, and PKC in Cell Membranes of Elderly Hypertensives Hypertension, January 1, 2003; 41(1): 176 - 182. [Abstract] [Full Text] [PDF]

				M. J. Brosnan, J. S. Clark, B. Jeffs, C. D. Negrin, P. Van Vooren, S. M. Arribas, H. Carswell, T. J. Aitman, C. Szpirer, I. M. Macrae, et al. Genes Encoding Atrial and Brain Natriuretic Peptides as Candidates for Sensitivity to Brain Ischemia in Stroke-Prone Hypertensive Rats Hypertension, January 1, 1999; 33(1): 290 - 297. [Abstract] [Full Text] [PDF]

				D. Sbrissa, A. Hajra, and K. N. Bitar Origin of molecular species of diacylglycerol induced by bombesin in smooth muscle cells from rabbit rectosigmoid Am J Physiol Gastrointest Liver Physiol, July 1, 1998; 275(1): G138 - G150. [Abstract] [Full Text] [PDF]

				Y. Ohya, N. Adachi, Y. Nakamura, M. Setoguchi, I. Abe, and M. Fujishima Stretch-Activated Channels in Arterial Smooth Muscle of Genetic Hypertensive Rats Hypertension, January 1, 1998; 31(1): 254 - 258. [Abstract] [Full Text] [PDF]