This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murasawa, S. Articles by Inada, M. Search for Related Content PubMed PubMed Citation Articles by Murasawa, S. Articles by Inada, M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB DEXAMETHASONE

(Hypertension. 1995;26:665-669.)
© 1995 American Heart Association, Inc.

Articles

Glucocorticoids Regulate V_1a Vasopressin Receptor Expression by Increasing mRNA Stability in Vascular Smooth Muscle Cells

Satoshi Murasawa; Hiroaki Matsubara; Kazuhisa Kizima; Katsuya Maruyama; Yasukiyo Mori; Mitsuo Inada

From the Second Department of Internal Medicine, Kansai Medical University, Osaka, Japan.

Correspondence to Hiroaki Matsubara, MD, PhD, Second Department of Internal Medicine, Kansai Medical University, Fumizonocho 1, Moriguchi, Osaka 570, Japan.

Abstract Enhancement of vascular responsiveness is considered to be one of the major contributing factors observed in glucocorticoid-induced hypertension. We examined the effects of glucocorticoids on V_1a arginine vasopressin receptor mRNA and protein levels in vascular smooth muscle cells. Dexamethasone (1 µmol/L) produced a 1.8-fold increase in V_1a receptor density without changing its affinity. Steady-state values of V_1a receptor mRNA, analyzed by Northern blotting, increased 2.7-fold after a 12-hour exposure to dexamethasone. This effect of dexamethasone was blocked by the glucocorticoid antagonist RU38486 and did not occur in the presence of the protein synthesis inhibitor cycloheximide. The V_1a receptor gene transcription rate, determined by nuclear run-off assays, was unchanged in cells treated with dexamethasone for 12 hours. Dexamethasone increased the half-life of V_1a receptor mRNA by 2.2-fold. These findings suggest that dexamethasone upregulates the expression of the V_1a receptor by increasing mRNA stability rather than by gene transcription and that de novo protein synthesis is involved in this regulation.

Key Words: RNA, messenger • receptors, vasopressin • gene expression • glucocorticoids

This article has been cited by other articles:

				S. Montero, H. Mendoza, V. Valles, M. Lemus, R. Alvarez-Buylla, and E. R. de Alvarez-Buylla Arginine-vasopressin mediates central and peripheral glucose regulation in response to carotid body receptor stimulation with Na-cyanide J Appl Physiol, June 1, 2006; 100(6): 1902 - 1909. [Abstract] [Full Text] [PDF]

				G. Horvath and A. Wanner Inhaled corticosteroids: effects on the airway vasculature in bronchial asthma Eur. Respir. J., January 1, 2006; 27(1): 172 - 187. [Abstract] [Full Text] [PDF]

				T. Kogai, Y. Kanamoto, A. I. Li, L. H. Che, E. Ohashi, K. Taki, R. A. Chandraratna, T. Saito, and G. A. Brent Differential Regulation of Sodium/Iodide Symporter Gene Expression by Nuclear Receptor Ligands in MCF-7 Breast Cancer Cells Endocrinology, July 1, 2005; 146(7): 3059 - 3069. [Abstract] [Full Text] [PDF]

				O. B. Vagnes, F. H. Hansen, R. E. F. Christiansen, C. Gjerstad, and B. M. Iversen Age-dependent regulation of vasopressin V1a receptors in preglomerular vessels from the spontaneously hypertensive rat Am J Physiol Renal Physiol, May 1, 2004; 286(5): F997 - F1003. [Abstract] [Full Text] [PDF]

				A. Lacroix, N. N'Diaye, J. Tremblay, and P. Hamet Ectopic and Abnormal Hormone Receptors in Adrenal Cushing's Syndrome Endocr. Rev., February 1, 2001; 22(1): 75 - 110. [Abstract] [Full Text]

				O. Vagnes, J. J. Feng, B. M. Iversen, and W. J. Arendshorst Upregulation of V1 receptors in renal resistance vessels of rats developing genetic hypertension Am J Physiol Renal Physiol, June 1, 2000; 278(6): F940 - F948. [Abstract] [Full Text] [PDF]

				M. E Ullian The role of corticosteroids in the regulation of vascular tone Cardiovasc Res, January 1, 1999; 41(1): 55 - 64. [Full Text] [PDF]

				C. Rabadan-Diehl, G. Makara, A. Kiss, S. Lolait, D. Zelena, T. Ochedalski, and G. Aguilera Regulation of Pituitary V1b Vasopressin Receptor Messenger Ribonucleic Acid by Adrenalectomy and Glucocorticoid Administration Endocrinology, December 1, 1997; 138(12): 5189 - 5194. [Abstract] [Full Text] [PDF]