(Hypertension. 1995;26:1173-1176.)
© 1995 American Heart Association, Inc.
Articles |
Stress-Induced Elevation of Ouabainlike Compound in Rat Plasma and Adrenal
From the Second Department of Internal Medicine, University of Tokyo (A.G., H.N., M.O.); Department of Human Dry Dock, Sanraku Hospital (K.Y.); and Department of Physiology, Osaka City University (Y.T.) (Japan).
Correspondence to Atsuo Goto, MD, Second Department of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan.
Abstract Recent observations demonstrate the presence of neurosteroids and their rapid increase in response to acute stress. In view of a steroidal nature of ouabainlike compound, we tested the hypothesis that ouabainlike compound may participate in a homeostatic response to acute stress. Male Wistar rats were subjected to acute stress by swimming in water (22°C) for 10 minutes. The levels of ouabainlike compound in plasma, hypothalamus, pituitary, and adrenal at 10, 40, and 70 minutes (n=8 for each) after the end of swim stress were compared with nonstressed control levels (n=10). Ouabainlike compound was measured by a radioimmunoassay for ouabain. Plasma levels of corticosterone and catecholamines were also measured. Plasma corticosterone concentrations increased rapidly at 10 minutes (P<.01) and then declined. A trend for a rise in plasma catecholamines was found at 10 minutes. Adrenal levels of ouabainlike compound concomitantly increased at 10 minutes (P<.01, control: 58.9±5.9 pmol ouabain equivalents per gram; 10 minutes: 92.5±4.8; 40 minutes: 47.3±9.6; 70 minutes: 45.1±6.3). In contrast, the response of plasma ouabainlike compound was slow and doubled at 40 minutes (P<.01, control: 115±12 pmol ouabain equivalents per liter; 10 minutes: 132±23; 40 minutes: 226±53; 70 minutes: 117±16). Ouabainlike compound levels in hypothalamus and pituitary remained unaltered. These findings suggest that ouabainlike compound may function as a stress hormone.
Key Words: stress • hypothalamo-hypophyseal system • hormones
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