This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Koike, G. Articles by Jacob, H. J. Search for Related Content PubMed PubMed Citation Articles by Koike, G. Articles by Jacob, H. J.

(Hypertension. 1995;26:998-1002.)
© 1995 American Heart Association, Inc.

Articles

Cloning, Characterization, and Genetic Mapping of the Rat Type 2 Angiotensin II Receptor Gene

George Koike; Eric S. Winer; Masatsugu Horiuchi; Donna M. Brown; Claude Szpirer; Victor J. Dzau; Howard J. Jacob

From the Cardiovascular Research Center, Massachusetts General Hospital–East (G.K., E.S.W., D.M.B., H.J.J.), Charlestown; the Falk Cardiovascular Research Center, Stanford University School of Medicine (M.H., VJ.D.), Stanford, Calif; and the Département de Biologie Moléculaire, Université Libre de Bruxelles (C.S.), Rhode-St-Genèse, Belgium.

Correspondence to Howard J. Jacob, PhD, Cardiovascular Research Center, Mail Code 1494201, Massachusetts General Hospital–East, 149 13th St, Charlestown, MA 02129-2060. E-mail jacob@helix.mgh.harvard.edu.

Abstract The renin-angiotensin system plays an important role in blood pressure homeostasis, but the contribution of the type 2 angiotensin II receptor (AT₂R) is still unclear. The reports that the AT₂R gene has been mapped to the X chromosome in human and rat and the previous report of a gene, Bp3, on the X chromosome responsible for an increase in blood pressure have suggested that the rat AT₂R gene (Agtr2) could be this gene. To elucidate whether Agtr2 is Bp3, Agtr2 was cloned. A simple sequence repeat in the 3'-flanking region of this gene was identified and used as a genetic marker to map Agtr2 to the X chromosome at 18.1 cM distal to the androgen receptor locus. This map position is outside the confidence interval reported for Bp3, demonstrating that Agtr2 cannot be Bp3. However, these data will enhance the research into the AT₂R biology as well as the study of the X chromosome.

Key Words: receptor, angiotensin • rats • cloning, molecular

This article has been cited by other articles:

				T. Yamada, A. Kuno, K. Masuda, K. Ogawa, M. Sogawa, S. Nakamura, T. Ando, H. Sano, T. Nakazawa, H. Ohara, et al. Candesartan, an Angiotensin II Receptor Antagonist, Suppresses Pancreatic Inflammation and Fibrosis in Rats J. Pharmacol. Exp. Ther., October 1, 2003; 307(1): 17 - 23. [Abstract] [Full Text] [PDF]

				R. Ozono, T. Matsumoto, T. Shingu, T. Oshima, Y. Teranishi, M. Kambe, H. Matsuura, G. Kajiyama, Z.-Q. Wang, A. F. Moore, et al. Expression and localization of angiotensin subtype receptor proteins in the hypertensive rat heart Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2000; 278(3): R781 - R789. [Abstract] [Full Text] [PDF]

				J. P. Rapp Genetic Analysis of Inherited Hypertension in the Rat Physiol Rev, January 1, 2000; 80(1): 135 - 172. [Abstract] [Full Text] [PDF]

				N. Miyata, F. Park, X. F. Li, and A. W. Cowley Jr. Distribution of angiotensin AT1 and AT2 receptor subtypes in the rat kidney Am J Physiol Renal Physiol, September 1, 1999; 277(3): F437 - F446. [Abstract] [Full Text] [PDF]

				M. Goto, M. Mukoyama, S.-i. Suga, T. Matsumoto, M. Nakagawa, R. Ishibashi, M. Kasahara, A. Sugawara, I. Tanaka, and K. Nakao Growth-Dependent Induction of Angiotensin II Type 2 Receptor in Rat Mesangial Cells Hypertension, September 1, 1997; 30(3): 358 - 362. [Abstract] [Full Text]