(Hypertension. 1996;27:552-557.)
© 1996 American Heart Association, Inc.
Articles |
cDNA Cloning and Gene Expression of Human Type I
cGMP-Dependent Protein Kinase
From the Department of Medicine and Clinical Science, Kyoto (Japan) University Graduate School of Medicine.
Correspondence to Hiroshi Itoh, MD, PhD, Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606, Japan. E-mail hiito@kuhp.kyoto-u.ac.jp.
Abstract The type I cGMP-dependent protein kinase (cGK) is
one of the major pathways for the cGMP cascade and has been
demonstrated to inhibit platelet aggregation, relax smooth muscle
cells, and control cardiocyte contractility.
There are two subtypes of the type I cGK, cGKI
and cGKIß. The
former is more sensitive to cGMP than the latter. In humans, cGKIß
cDNA was isolated, but the full structure and tissue-specific gene
expression of cGKI have not been determined. The significance of cGK
in human cardiovascular diseases has not been
investigated at the molecular level. In the present study, we
isolated the full-length human cGKI cDNA (-36 to +2177;
the translation start site: +1) encoding the 671–amino acid
protein.
Nucleotides +267 to +2177 of the isolated cDNA were
identical to the corresponding nucleotides of human cGKIß
cDNA. Southern blot analysis suggested that human cGKI and
cGKIß are generated by alternative splicing of a single gene assigned
to chromosome 10. By Northern blot analysis, we detected
abundant human cGKI mRNA (7.0 kb) in the aorta, heart, kidneys, and
adrenals. In contrast, human cGKIß mRNA (7.0 kb) was detected
abundantly only in the uterus. In cultured vascular smooth muscle
cells, the type I cGK mRNA concentration was reduced to 10% of the
basal level by 4x10-10 mol/L
platelet-derived growth factor. Angiotensin II
(10-8 mol/L), transforming growth
factor-ß (4x10-11 mol/L), and tumor
necrosis factor- (6x10-6 mol/L) also
exhibited an inhibitory effect on type I cGK gene
expression. These findings suggest a
pathophysiological implication of the type I
cGK in cardiovascular diseases, including hypertension
and atherosclerosis.
Key Words: protein kinases • cloning, molecular • muscle, smooth, vascular • platelet-derived growth factor • angiotensin II • growth substances
This article has been cited by other articles:
 |
|
 |
|
  H. K. Surks cGMP-Dependent Protein Kinase I and Smooth Muscle Relaxation: A Tale of Two Isoforms Circ. Res., November 26, 2007; 101(11): 1078 - 1080. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zeng, S. Zhuang, J. Gloddek, C.-C. Tseng, G. R. Boss, and R. B. Pilz Regulation of cGMP-dependent Protein Kinase Expression by Rho and Kruppel-like Transcription Factor-4 J. Biol. Chem., June 23, 2006; 281(25): 16951 - 16961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Taniguchi, Y. L. Kwak, K. A. Jones, D. O. Warner, and W. J. Perkins Nitric oxide sensitivity in pulmonary artery and airway smooth muscle: a possible role for cGMP responsiveness Am J Physiol Lung Cell Mol Physiol, May 1, 2006; 290(5): L1018 - L1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sellak, C. Choi, N. Browner, and T. M. Lincoln Upstream Stimulatory Factors (USF-1/USF-2) Regulate Human cGMP-dependent Protein Kinase I Gene Expression in Vascular Smooth Muscle Cells J. Biol. Chem., May 6, 2005; 280(18): 18425 - 18433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yamamoto and N. Suzuki Expression and Function of cGMP-dependent Protein Kinase Type I during Medaka Fish Embryogenesis J. Biol. Chem., April 29, 2005; 280(17): 16979 - 16986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chang, J. A. Hypolite, M. Velez, A. Changolkar, A. J. Wein, S. Chacko, and M. E. DiSanto Downregulation of cGMP-dependent protein kinase-1 activity in the corpus cavernosum smooth muscle of diabetic rabbits Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R950 - R960. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. C. Browner, H. Sellak, and T. M. Lincoln Downregulation of cGMP-dependent protein kinase expression by inflammatory cytokines in vascular smooth muscle cells Am J Physiol Cell Physiol, July 1, 2004; 287(1): C88 - C96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. L. O. Buxton Regulation of Uterine Function: a Biochemical Conundrum in the Regulation of Smooth Muscle Relaxation Mol. Pharmacol., May 1, 2004; 65(5): 1051 - 1059. [Abstract] [Full Text]
|
|
|
|
 |
|
 X.-H. Jin, H. E. McGrath, J. J. Gildea, H. M. Siragy, R. A. Felder, and R. M. Carey Renal Interstitial Guanosine Cyclic 3', 5'-Monophosphate Mediates Pressure-Natriuresis Via Protein Kinase G Hypertension, May 1, 2004; 43(5): 1133 - 1139. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Richie-Jannetta, S. H. Francis, and J. D. Corbin Dimerization of cGMP-dependent Protein Kinase I{beta} Is Mediated by an Extensive Amino-terminal Leucine Zipper Motif, and Dimerization Modulates Enzyme Function J. Biol. Chem., December 12, 2003; 278(50): 50070 - 50079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Schroder, G. Klein, B. Fiedler, M. Bastein, N. Schnasse, A. Hillmer, S. Ames, S. Gambaryan, H. Drexler, U. Walter, et al. Single L-type Ca2+ channel regulation by cGMP-dependent protein kinase type I in adult cardiomyocytes from PKG I transgenic mice Cardiovasc Res, November 1, 2003; 60(2): 268 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gerzanich, A. Ivanov, S. Ivanova, J. B. Yang, H. Zhou, Y. Dong, and J. M. Simard Alternative Splicing of cGMP-Dependent Protein Kinase I in Angiotensin-Hypertension: Novel Mechanism for Nitrate Tolerance in Vascular Smooth Muscle Circ. Res., October 31, 2003; 93(9): 805 - 812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wang, X. Wu, T. M. Lincoln, and J. E. Murphy-Ullrich Expression of Constitutively Active cGMP-Dependent Protein Kinase Prevents Glucose Stimulation of Thrombospondin 1 Expression and TGF-{beta} Activity Diabetes, August 1, 2003; 52(8): 2144 - 2150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yamahara, H. Itoh, T.-H. Chun, Y. Ogawa, J. Yamashita, N. Sawada, Y. Fukunaga, M. Sone, T. Yurugi-Kobayashi, K. Miyashita, et al. Significance and therapeutic potential of the natriuretic peptides/cGMP/cGMP-dependent protein kinase pathway in vascular regeneration PNAS, March 18, 2003; 100(6): 3404 - 3409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Murthy and H. Zhou Selective phosphorylation of the IP3R-I in vivo by cGMP-dependent protein kinase in smooth muscle Am J Physiol Gastrointest Liver Physiol, February 1, 2003; 284(2): G221 - G230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sellak, X. Yang, X. Cao, T. Cornwell, G. A. Soff, and T. Lincoln Sp1 Transcription Factor as a Molecular Target for Nitric Oxide- and Cyclic Nucleotide-Mediated Suppression of cGMP-Dependent Protein Kinase-I{alpha} Expression in Vascular Smooth Muscle Cells Circ. Res., March 8, 2002; 90(4): 405 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Lincoln, N. Dey, and H. Sellak Signal Transduction in Smooth Muscle: Invited Review: cGMP-dependent protein kinase signaling mechanisms in smooth muscle: from the regulation of tone to gene expression J Appl Physiol, September 1, 2001; 91(3): 1421 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Huminiecki and R. Bicknell In Silico Cloning of Novel Endothelial-Specific Genes Genome Res., November 1, 2000; 10(11): 1796 - 1806. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-g. Wang, M. B. Wagner, R. W. Joyner, and R. Kumar cGMP-dependent protein kinase mediates stimulation of L-type calcium current by cGMP in rabbit atrial cells Cardiovasc Res, November 1, 2000; 48(2): 310 - 322. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Lucas, G. M. Pitari, S. Kazerounian, I. Ruiz-Stewart, J. Park, S. Schulz, K. P. Chepenik, and S. A. Waldman Guanylyl Cyclases and Signaling by Cyclic GMP Pharmacol. Rev., September 1, 2000; 52(3): 375 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D. Browning, M. P. McShane, C. Marty, and R. D. Ye Nitric Oxide Activation of p38 Mitogen-activated Protein Kinase in 293T Fibroblasts Requires cGMP-dependent Protein Kinase J. Biol. Chem., January 28, 2000; 275(4): 2811 - 2816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kumar, R. W. Joyner, P. Komalavilas, and T. M. Lincoln Analysis of Expression of cGMP-Dependent Protein Kinase in Rabbit Heart Cells J. Pharmacol. Exp. Ther., December 1, 1999; 291(3): 967 - 975. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. K. Surks, N. Mochizuki, Y. Kasai, S. P. Georgescu, K. M. Tang, M. Ito, T. M. Lincoln, and M. E. Mendelsohn Regulation of Myosin Phosphatase by a Specific Interaction with cGMP- Dependent Protein Kinase I Science, November 19, 1999; 286(5444): 1583 - 1587. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ruetten, U. Zabel, W. Linz, and H. H. H. W. Schmidt Downregulation of Soluble Guanylyl Cyclase in Young and Aging Spontaneously Hypertensive Rats Circ. Res., September 17, 1999; 85(6): 534 - 541. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Idriss, T. Gudi, D. E. Casteel, V. G. Kharitonov, R. B. Pilz, and G. R. Boss Nitric Oxide Regulation of Gene Transcription via Soluble Guanylate Cyclase and Type I cGMP-dependent Protein Kinase J. Biol. Chem., April 2, 1999; 274(14): 9489 - 9493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Collins and M. D. Uhler Cyclic AMP- and Cyclic GMP-dependent Protein Kinases Differ in Their Regulation of Cyclic AMP Response Element-dependent Gene Transcription J. Biol. Chem., March 26, 1999; 274(13): 8391 - 8404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-D. Chiche, S. M. Schlutsmeyer, D. B. Bloch, S. M. de la Monte, J. D. Roberts Jr., G. Filippov, S. P. Janssens, A. Rosenzweig, and K. D. Bloch Adenovirus-mediated Gene Transfer of cGMP-dependent Protein Kinase Increases the Sensitivity of Cultured Vascular Smooth Muscle Cells to the Antiproliferative and Pro-apoptotic Effects of Nitric Oxide/cGMP J. Biol. Chem., December 18, 1998; 273(51): 34263 - 34271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sellak, X. Yang, X. Cao, T. Cornwell, G. A. Soff, and T. Lincoln Sp1 Transcription Factor as a Molecular Target for Nitric Oxide- and Cyclic Nucleotide-Mediated Suppression of cGMP-Dependent Protein Kinase-I{alpha} Expression in Vascular Smooth Muscle Cells Circ. Res., March 8, 2002; 90(4): 405 - 412. [Abstract] [Full Text] [PDF]
|
|