(Hypertension. 1996;28:91-97.)
© 1996 American Heart Association, Inc.
Articles |
Angiotensin II Type 2 Receptor mRNA Expression in the Developing Cardiopulmonary System of the Rat
Institut National de la Sante et de la Recherche Medicale (INSERM) U36, and Laboratoire de Medecine Experimentale, College de France, Paris.
Correspondence to Jean-Marie Gasc, INSERM U36, College de France, 3 rue d'Ulm, 75005 Paris, France.
Recent studies have shown that angiotensin II has a trophic action on the heart. The presence of two types of angiotensin II receptors, type 1 (AT1) and type 2 (AT2), has been reported in the rat heart. This in situ hybridization study describes the tissue and cell location of AT2 receptor mRNA in the developing rat cardiopulmonary system, from 15 days of gestation to adulthood. Expression of AT1A receptor mRNA was studied in parallel for direct comparison. The aortic arch and pulmonary artery expressed high levels of AT2 receptor mRNA from 15 days of gestation up until 15 days postpartum, whereas expression of this mRNA was observed only just before and after birth in the coronary arteries. AT2 receptor mRNA was not detected in any cardiac muscle of the fetus, neonate, or adult. The annulus of all four heart valves expressed AT2 mRNA from 21 days of gestation until 10 days postpartum, but no labeling was seen in the valve leaflets. The subendocardial atrial tissue showed a high level of AT2 receptor mRNA expression during the early postnatal period, but no expression was observed in the atrial myocytes from fetal stages to adulthood. The bronchi and trachea, but not the lung parenchyma, showed a high level of AT2 receptor mRNA expression starting from 17 days of gestation until 10 days postpartum. AT2 receptor mRNA expression in the cardiopulmonary system is therefore transient, developmentally regulated, and mostly located in vascular structures. By these three characteristics, its expression contrasts with that of AT1A, which is continuously expressed in the cardiac muscle to adulthood. This spatiotemporal pattern of expression of angiotensin II receptor mRNAs during development suggests a possible role for angiotensin II in organogenesis.
Key Words: receptors, angiotensin II • heart • in situ hybridization • gene expression
This article has been cited by other articles:
 |
|
 |
|
  P. K. Mehta and K. K. Griendling Angiotensin II cell signaling: physiological and pathological effects in the cardiovascular system Am J Physiol Cell Physiol, January 1, 2007; 292(1): C82 - C97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Reini, C. E. Wood, E. Jensen, and M. Keller-Wood Increased maternal cortisol in late-gestation ewes decreases fetal cardiac expression of 11beta-HSD2 mRNA and the ratio of AT1 to AT2 receptor mRNA Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1708 - R1716. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Miyawaki, T Okutani, R Higuchi, and N Yoshikawa Plasma angiotensin II concentrations in the early neonatal period Arch. Dis. Child. Fetal Neonatal Ed., September 1, 2006; 91(5): F359 - F362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W.J.H. Dielis, M. Smid, H. M.H. Spronk, K. Hamulyak, A. A. Kroon, H. ten Cate, and P. W. de Leeuw The Prothrombotic Paradox of Hypertension: Role of the Renin-Angiotensin and Kallikrein-Kinin Systems Hypertension, December 1, 2005; 46(6): 1236 - 1242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Okumura, M. Iwai, A. Ide, M. Mogi, M. Ito, and M. Horiuchi Sex Difference in Vascular Injury and the Vasoprotective Effect of Valsartan Are Related to Differential AT2 Receptor Expression Hypertension, September 1, 2005; 46(3): 577 - 583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Perlegas, H. Xie, S. Sinha, A. V. Somlyo, and G. K. Owens ANG II type 2 receptor regulates smooth muscle growth and force generation in late fetal mouse development Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H96 - H102. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nishimura, Y. Yang, C. Hubert, J.-M. Gasc, K. Ruijtenbeek, J. De Mey, H. A. J. S. Boudier, and P. Corvol Maturation-dependent changes of angiotensin receptor expression in fowl Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2003; 285(1): R231 - R242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Li, M. Iwai, L. Wu, T. Shiuchi, T. Jinno, T.-X. Cui, and M. Horiuchi Role of AT2 receptor in the brain in regulation of blood pressure and water intake Am J Physiol Heart Circ Physiol, January 1, 2003; 284(1): H116 - H121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Z. Su, N. Fukuda, X.-Q. Jin, Y.-M. Lai, R. Suzuki, Y. Tahira, H. Takagi, Y. Ikeda, K. Kanmatsuse, and H. Miyazaki Effect of AT2 Receptor on Expression of AT1 and TGF-{beta} Receptors in VSMCs from SHR Hypertension, December 1, 2002; 40(6): 853 - 858. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Suzuki, M. Iwai, H. Nakagami, L. Wu, R. Chen, T. Sugaya, M. Hamada, K. Hiwada, and M. Horiuchi Role of Angiotensin II-Regulated Apoptosis Through Distinct AT1 and AT2 Receptors in Neointimal Formation Circulation, August 13, 2002; 106(7): 847 - 853. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. P. Bagby, L. S. LeBard, Z. Luo, R. C. Speth, B. E. Ogden, and C. L. Corless Angiotensin II Type 1 and 2 Receptors in Conduit Arteries of Normal Developing Microswine Arterioscler Thromb Vasc Biol, July 11, 2002; 22(7): 1113 - 1121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Jin, N. Fukuda, J.-Z. Su, Y.-M. Lai, R. Suzuki, Y. Tahira, H. Takagi, Y. Ikeda, K. Kanmatsuse, and H. Miyazaki Angiotensin II Type 2 Receptor Gene Transfer Downregulates Angiotensin II Type 1a Receptor in Vascular Smooth Muscle Cells Hypertension, May 1, 2002; 39(5): 1021 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Berry, R. Touyz, A. F. Dominiczak, R. C. Webb, and D. G. Johns Angiotensin receptors: signaling, vascular pathophysiology, and interactions with ceramide Am J Physiol Heart Circ Physiol, December 1, 2001; 281(6): H2337 - H2365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Varagic, D. Susic, and E. D. Frohlich Coronary Hemodynamic and Ventricular Responses to Angiotensin Type 1 Receptor Inhibition in SHR : Interaction With Angiotensin Type 2 Receptors Hypertension, June 1, 2001; 37(6): 1399 - 1403. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-X. Cui, H. Nakagami, M. Iwai, Y. Takeda, T. Shiuchi, L. Daviet, C. Nahmias, and M. Horiuchi Pivotal role of tyrosine phosphatase SHP-1 in AT2 receptor-mediated apoptosis in rat fetal vascular smooth muscle cell Cardiovasc Res, March 1, 2001; 49(4): 863 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Malendowicz, P. V. Ennezat, M. Testa, L. Murray, E. H. Sonnenblick, T. Evans, and T. H. LeJemtel Angiotensin II Receptor Subtypes in the Skeletal Muscle Vasculature of Patients With Severe Congestive Heart Failure Circulation, October 31, 2000; 102(18): 2210 - 2213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsumoto, R. Ozono, T. Oshima, H. Matsuura, T. Sueda, G. Kajiyama, and M. Kambe Type 2 angiotensin II receptor is downregulated in cardiomyocytes of patients with heart failure Cardiovasc Res, April 1, 2000; 46(1): 73 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Chassagne, S. Eddahibi, C. Adamy, D. Rideau, F. Marotte, J.-L. Dubois-Randé, S. Adnot, J.-L. Samuel, and E. Teiger Modulation of Angiotensin II Receptor Expression during Development and Regression of Hypoxic Pulmonary Hypertension Am. J. Respir. Cell Mol. Biol., March 1, 2000; 22(3): 323 - 332. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. AKISHITA, M. HORIUCHI, H. YAMADA, L. ZHANG, G. SHIRAKAMI, K. TAMURA, Y. OUCHI, and V. J. DZAU Inflammation influences vascular remodeling through AT2 receptor expression and signaling Physiol Genomics, January 24, 2000; 2(1): 13 - 20. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Carey, Z.-Q. Wang, and H. M. Siragy Role of the Angiotensin Type 2 Receptor in the Regulation of Blood Pressure and Renal Function Hypertension, January 1, 2000; 35(1): 155 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C Wollert and H. Drexler The renin-angiotensin system and experimental heart failure Cardiovasc Res, September 1, 1999; 43(4): 838 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lamparter, Y. Sun, and K. T Weber Angiotensin II receptor blockade during gestation attenuates collagen formation in the developing rat heart Cardiovasc Res, July 1, 1999; 43(1): 165 - 172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamada, M. Akishita, M. Ito, K. Tamura, L. Daviet, J. Y. A. Lehtonen, V. J. Dzau, and M. Horiuchi AT2 Receptor and Vascular Smooth Muscle Cell Differentiation in Vascular Development Hypertension, June 1, 1999; 33(6): 1414 - 1419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horiuchi, M. Akishita, and V. J. Dzau Recent Progress in Angiotensin II Type 2 Receptor Research in the Cardiovascular System Hypertension, February 1, 1999; 33(2): 613 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Matsubara Pathophysiological Role of Angiotensin II Type 2 Receptor in Cardiovascular and Renal Diseases Circ. Res., December 14, 1998; 83(12): 1182 - 1191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Q. Wang, A. F. Moore, R. Ozono, H. M. Siragy, and R. M. Carey Immunolocalization of Subtype 2 Angiotensin II (AT2) Receptor Protein in Rat Heart Hypertension, July 1, 1998; 32(1): 78 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wharton, K. Morgan, R. A. D. Rutherford, J. D. Catravas, A. Chester, B. F. Whitehead, M. R. D. Leval, M. H. Yacoub, and J. M. Polak Differential Distribution of Angiotensin AT2 Receptors in the Normal and Failing Human Heart J. Pharmacol. Exp. Ther., January 1, 1998; 284(1): 323 - 336. [Abstract] [Full Text]
|
|
|
|
|
|
R. Ozono, Z.-Q. Wang, A. F. Moore, T. Inagami, H. M. Siragy, and R. M. Carey Expression of the Subtype 2 Angiotensin (AT2) Receptor Protein in Rat Kidney Hypertension, November 1, 1997; 30(5): 1238 - 1246. [Abstract] [Full Text]
|
|
|
|
|
|
C. Chassagne, C. Adamy, P. Ratajczak, B. Gingras, E. Teiger, E. Planus, P. Oliviero, L. Rappaport, J.-L. Samuel, and S. Meloche Angiotensin II AT2 receptor inhibits smooth muscle cell migration via fibronectin cell production and binding Am J Physiol Cell Physiol, April 1, 2002; 282(4): C654 - C664. [Abstract] [Full Text] [PDF]
|
|