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(Hypertension. 1996;28:209-212.)
© 1996 American Heart Association, Inc.

Articles

Effects of Insulin on Calcium Metabolism and Platelet Aggregation

Mari Ishida; Takafumi Ishida; Norihisa Ono; Hideo Matsuura; Mitsuaki Watanabe; Goro Kajiyama; Masayuki Kambe; Tetsuya Oshima

the First Department of Internal Medicine (M.I., T.I., N.O., H.M., M.W., G.K.) and Department of Clinical Laboratory Medicine (M.K., T.O.), Hiroshima (Japan) University School of Medicine.

Correspondence to Tetsuya Oshima, MD, Department of Clinical Laboratory Medicine, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734, Japan.

The influence of insulin on platelets in vitro has not been exhaustively investigated. To clarify whether insulin affects Ca²⁺ metabolism in platelets directly or through alteration of other systems regulating intracellular Ca²⁺ homeostasis, we examined the effect of insulin both alone and in combination with prostaglandin E₁ on platelet aggregation and Ca²⁺ metabolism. Incubation of rat platelets with insulin reduced thrombin-induced Ca²⁺ influx but did not change thrombin-evoked release of Ca²⁺ from internal stores or the size of internal Ca²⁺ stores. The interactive effects of insulin with prostaglandin E₁ were only additive, and insulin did not augment the effects of prostaglandin E₁ on platelet Ca²⁺ metabolism. In contrast, insulin did not inhibit thrombin-induced platelet aggregation but did augment inhibition of platelet aggregation by prostaglandin E₁. Our results suggest that insulin inhibits platelet function by both prostaglandin E₁–dependent and -independent mechanisms.

Key Words: insulin • calcium • prostaglandins • platelet aggregation

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