This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hirata, Y. Articles by Omata, M. Search for Related Content PubMed PubMed Citation Articles by Hirata, Y. Articles by Omata, M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB NITRIC OXIDE PROSTAGLANDIN F2ALPHA

(Hypertension. 1997;29:58.)
© 1997 American Heart Association, Inc.

Research Articles (Issue 1, Part 1)

Receptor Subtype for Vasopressin-Induced Release of Nitric Oxide From Rat Kidney

Yasunobu Hirata; Hiroshi Hayakawa; Masao Kakoki; Akihiro Tojo; Etsu Suzuki; Daisuke Nagata; Kenjiro Kimura; Atsuo Goto; Kazuya Kikuchi; Tetsuo Nagano; Masaaki Hirobe; Masao Omata

The Second Department of Internal Medicine (Y.H., H.H., M.K., A.T., E.S., D.N., K.K, A.G., M.O.) and Faculty of Pharmaceutical Sciences (K.K., T.N., M.H.), University of Tokyo (Japan).

Correspondence to Yasunobu Hirata, MD, The Second Department of Internal Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan. E-mail hirata-2im@h.u-tokyo.ac.jp

The vasopressin receptor subtype that causes nitric oxide (NO) release remains controversial. To elucidate this receptor-ligand interaction, we examined the effects of vasopressin receptor antagonists on vasopressin-induced release of NO from isolated perfused rat kidneys by using a sensitive chemiluminescence assay. Vasopressin increased renal perfusion pressure and NO signals in the perfusate in a dose-dependent manner. N^G-Monomethyl-L-arginine abolished this increase in NO release; however, a similar increase in renal perfusion pressure induced by prostaglandin F₂ was not associated with the increase in NO release. OPC-21268, a V₁ receptor antagonist, significantly reduced the vasopressin-evoked renal vasoconstriction and NO release, whereas OPC-31260, a V₂ receptor antagonist, had no effects. Moreover, desmopressin, a selective V₂ receptor agonist, did not increase the NO signal. NO release by vasopressin was markedly attenuated in deoxycorticosterone acetate (DOCA)–salt hypertensive rat kidneys compared with control kidneys (10^-10 mol/L vasopressin: +0.8±0.3 versus +6.9±1.4 fmol/min per gram kidney, DOCA versus control; P<.001). Histochemical analysis for renal NO synthase revealed a substantial attenuation of the staining of endothelial NO synthase in DOCA-salt rats. These results directly demonstrate that vasopressin stimulates NO release via the endothelial V₁ receptor in the rat kidney.

Key Words: endothelium • hormone antagonists • desoxycorticosterone • nitric oxide

This article has been cited by other articles:

				N. Gassanov, M. Jankowski, B. Danalache, D. Wang, R. Grygorczyk, U. C. Hoppe, and J. Gutkowska Arginine Vasopressin-mediated Cardiac Differentiation: INSIGHTS INTO THE ROLE OF ITS RECEPTORS AND NITRIC OXIDE SIGNALING J. Biol. Chem., April 13, 2007; 282(15): 11255 - 11265. [Abstract] [Full Text] [PDF]

				T. J. Scislo and D. S. O'Leary Vasopressin V1 receptors contribute to hemodynamic and sympathoinhibitory responses evoked by stimulation of adenosine A2a receptors in NTS Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1889 - H1898. [Abstract] [Full Text] [PDF]

				B. Yuan and A. W. Cowley Jr Evidence That Reduced Renal Medullary Nitric Oxide Synthase Activity of Dahl S Rats Enables Small Elevations of Arginine Vasopressin to Produce Sustained Hypertension Hypertension, February 1, 2001; 37(2): 524 - 528. [Abstract] [Full Text] [PDF]

				C. Loichot, C. Cazaubon, M. Grima, W. De Jong, D. Nisato, J.-L. Imbs, and M. Barthelmebs Vasopressin Does not Effect Hypertension Caused by Long-Term Nitric Oxide Inhibition Hypertension, February 1, 2000; 35(2): 602 - 608. [Abstract] [Full Text] [PDF]

				M. Kakoki, Y. Hirata, H. Hayakawa, A. Tojo, D. Nagata, E. Suzuki, K. Kimura, A. Goto, K. Kikuchi, T. Nagano, et al. Effects of Hypertension, Diabetes Mellitus, and Hypercholesterolemia on Endothelin Type B Receptor–Mediated Nitric Oxide Release From Rat Kidney Circulation, March 9, 1999; 99(9): 1242 - 1248. [Abstract] [Full Text] [PDF]

				T. Umino, E. Kusano, S. Muto, T. Akimoto, S. Yanagiba, S. Ono, M. Amemiya, Y. Ando, S. Homma, U. Ikeda, et al. AVP inhibits LPS- and IL-1beta -stimulated NO and cGMP via V1 receptor in cultured rat mesangial cells Am J Physiol Renal Physiol, March 1, 1999; 276(3): F433 - F441. [Abstract] [Full Text] [PDF]

				F. Park, A.-P. Zou, and A. W. Cowley Jr Arginine Vasopressin–Mediated Stimulation of Nitric Oxide Within the Rat Renal Medulla Hypertension, November 1, 1998; 32(5): 896 - 901. [Abstract] [Full Text] [PDF]