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Hypertension, Vol 3, 81-86, Copyright © 1981 by American Heart Association

ARTICLES

Purification of tonin by affinity chromatography

M Ikeda, J Gutkowska, G Thibault, R Boucher and J Genest

Tonin has been purified from rat submaxillary glands. The purification procedure included affinity chromatography on Sepharose 4B coupled to antitonin followed by DEAE chromatography and gel filtration on Sephadex G-100. Homogeneity of the purified enzyme was confirmed by Sephadex G-100 gel filtration, disc electrophoresis, and isoelectric focusing on polyacrylamide gel, immunodiffusion, and immunoelectrophoresis. The tonin was purified 11.5-fold, with 35% recovery. The purified tonin has full enzymatic or immunological activity.

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