From the Division of Hypertension and Vascular Research, Henry Ford
Hospital, Detroit, Mich.
Correspondence to Jeffrey L. Garvin, Division of Hypertension and Vascular Research, Henry Ford Hospital, 2799 W Grand Blvd, Detroit, MI 48202.
Abstract—Bradykinin plays an
important role in the regulation of renal hemodynamics.
However, there have been few studies of the effect of bradykinin on
isolated afferent arterioles, vascular segments that are important for
the regulation of renal blood flow and glomerular
filtration rate. Our purpose was to study (1) the effects of bradykinin
on isolated perfused rabbit afferent arterioles and (2) the mechanisms
of actions. Afferent arterioles dissected from rabbits were perfused in
vitro at 60 mm Hg. In afferent arterioles preconstricted with
phenylephrine, 10-12 to 10-10
mol/L bradykinin increased luminal diameter from 9.0±1.0 to
14.3±1.2 µm (P<0.003). In contrast,
10-9 and 10-8 mol/L bradykinin decreased
luminal diameter to 10.8±1.4 and 9.7±1.2 µm, respectively
(P<0.001). Bradykinin added to the bath had no effect
on preconstricted afferent arterioles. The addition of
[des-Arg9]-bradykinin (10-9 and
10-8 mol/L), a B1 receptor agonist, to the
lumen decreased diameter from 9.7±1.2 to 6.7±1.2 µm at
10-8 mol/L (P<0.002). Icatibant (Hoe 140),
a B2 receptor antagonist, blocked both the
vasodilation and vasoconstriction induced by bradykinin as well as the
vasoconstriction induced by [des-Arg9]-bradykinin. L-NAME
had no effect on bradykinin-induced dilation or constriction.
Indomethacin blocked both the dilation induced by
10-12 to 10-10 mol/L bradykinin and the
constriction induced by 10-9 to 10-8 mol/L
bradykinin. In fact, in the presence of indomethacin,
10-9 and 10-8 mol/L bradykinin increased
luminal diameter from 6.2±0.7 to 10.7±0.6 µm at
10-8 mol/L (P<0.001), which was attenuated
by L-NAME. Finally, in the presence of SQ29548, a
prostaglandin H2/thromboxane
A2 receptor antagonist, bradykinin caused
dilation at all concentrations tested. In conclusion, bradykinin has a
biphasic effect on afferent arterioles. Both dilation and constriction
may be mediated by bradykinin B2 receptors. The mechanisms
of vasodilation and vasoconstriction are due to
cyclooxygenase products, not nitric oxide.
© 1998 American Heart Association, Inc.
Scientific Contributions
Biphasic Effect of Bradykinin on Rabbit Afferent Arterioles
Key Words: prostaglandins • nitric oxide • renal blood flow • receptors • thromboxane
This article has been cited by other articles:
 |
|
 |
|
  B.#3.;e. B\#261;dzy\#324;ska and J. Sadowski Differential action of bradykinin on intrarenal regional perfusion in the rat: waning effect in the cortex and major impact in the medulla J. Physiol., August 1, 2009; 587(15): 3943 - 3953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zhang, N. Bhola, S. Kalyankrishna, W. Gooding, J. Hunt, R. Seethala, J. R. Grandis, and J. M. Siegfried Kinin B2 Receptor Mediates Induction of Cyclooxygenase-2 and Is Overexpressed in Head and Neck Squamous Cell Carcinomas Mol. Cancer Res., December 1, 2008; 6(12): 1946 - 1956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wang, G. Trottier, and R. Loutzenhiser Determinants of renal afferent arteriolar actions of bradykinin: evidence that multiple pathways mediate responses attributed to EDHF Am J Physiol Renal Physiol, September 1, 2003; 285(3): F540 - F549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Patel and F. G. Smith Age-dependent renal responses to the bradykinin B2- receptor antagonist icatibant in conscious lambs Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2001; 281(4): R1311 - R1318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bergaya, P. Meneton, M. Bloch-Faure, E. Mathieu, F. Alhenc-Gelas, B. I. Levy, and C. M. Boulanger Decreased Flow-Dependent Dilation in Carotid Arteries of Tissue Kallikrein-Knockout Mice Circ. Res., March 30, 2001; 88(6): 593 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. SCHANSTRA, M. E. MARIN-CASTAÑO, F. PRADDAUDE, I. TACK, J.-L. ADER, J.-P. GIROLAMI, J.-L. BASCANDS, and B. JEUNIER Bradykinin B1 Receptor-Mediated Changes in Renal Hemodynamics during Endotoxin-Induced Inflammation J. Am. Soc. Nephrol., July 1, 2000; 11(7): 1208 - 1215. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. R. Nurkiewicz and M. A. Boegehold Reinforcement of arteriolar myogenic activity by endogenous ANG II: susceptibility to dietary salt Am J Physiol Heart Circ Physiol, July 1, 2000; 279(1): H269 - H278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Ungvari, E. Sarkadi-Nagy, Z. Bagi, L. Szollar, and A. Koller Simultaneously Increased TxA2 Activity in Isolated Arterioles and Platelets of Rats With Hyperhomocysteinemia Arterioscler Thromb Vasc Biol, May 1, 2000; 20(5): 1203 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. G. Fischer, M. W. Hollmann, D. J. Horstman, and G. F. Rich Cyclooxygenase Inhibitors Attenuate Bradykinin-Induced Vasoconstriction in Septic Isolated Rat Lungs Anesth. Analg., March 1, 2000; 90(3): 625 - 631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. MATSUDA, K. HAYASHI, K. ARAKAWA, M. NAITOH, E. KUBOTA, M. HONDA, A. MATSUMOTO, H. SUZUKI, T. YAMAMOTO, F. KAJIYA, et al. Zonal Heterogeneity in Action of Angiotensin-Converting Enzyme Inhibitor on Renal Microcirculation: Role of Intrarenal Bradykinin J. Am. Soc. Nephrol., November 1, 1999; 10(11): 2272 - 2282. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Marcic, P. A. Deddish, H. L. Jackman, and E. G. Erdos Enhancement of Bradykinin and Resensitization of Its B2 Receptor Hypertension, March 1, 1999; 33(3): 835 - 843. [Abstract] [Full Text] [PDF]
|
|