(Hypertension. 1999;33:96-101.)
© 1999 American Heart Association, Inc.
Scientific Contributions |
Differential Regulation of Renal Angiotensin Subtype AT1A and AT2 Receptor Protein in Rats With Angiotensin-Dependent Hypertension
From the Departments of Medicine (Z.Q.W., N.T.H., H.M.S., R.M.C.) and Anesthesiology (L.J.M., R.A.J.), University of Virginia Health Sciences Center, Charlottesville.
Correspondence to Robert M. Carey, MD, Box 395, University of Virginia Health Sciences Center, Charlottesville, VA 22908. E-mail RMC4C{at}virginia.edu
Abstract—This study was designed to investigate distribution and regulation of the renal AT1A and AT2 subtype receptors in rats with either systemic angiotensin II (Ang II)–induced hypertension or acute phase renal hypertension (2-kidney, 1-clip [2K1C] or 2-kidney, 1-figure-of-8-wrap [2K1W]). In normal rat kidneys, positive immunostaining for the AT1A receptor was observed in the intrarenal vasculature, glomeruli, proximal and distal tubules, and collecting ducts. The AT2 receptor was localized mainly to the glomeruli. The AT1A but not AT2 receptor protein expression was significantly reduced in rats with 10-day systemic Ang II–induced hypertension. In both 7-day 2K1C and 3-day 2K1W rats, the AT1A receptor was significantly reduced in ischemic and contralateral kidneys compared with sham-operated control rats. Reduction in AT2 receptor expression was observed only in the ischemic kidneys in 2K1C and 2K1W renal hypertensive rats. These results demonstrate that the AT1A receptor is widely distributed in the glomerulus and all other nephron segments of the rat kidney. Renal AT1A but not AT2 receptor protein is downregulated in rats with Ang II–induced hypertension. In renal hypertensive rats, the AT1A receptor is bilaterally downregulated and the AT2 receptor is downregulated only in the ischemic kidney.
Key Words: receptors, angiotensin II • immunohistochemistry • kidney • hypertension, renal
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