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Hypertension. 1999;33:708-712

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(Hypertension. 1999;33:708-712.)
© 1999 American Heart Association, Inc.

Scientific Contributions

{alpha}1-Adrenergic Receptor Subtypes in Human Peripheral Blood Lymphocytes

Alberto Ricci; Elena Bronzetti; Andrea Conterno; Stefania Greco; Paolo Mulatero; Marina Schena; Domenica Schiavone; Seyed K. Tayebati; Franco Veglio; Francesco Amenta

From the Department of Cardiovascular and Respiratory Sciences (A.R., E.B, S.G.), "La Sapienza" University, Rome; Department of Medicine and Experimental Oncology, Chair of Internal Medicine (A.C., P.M., M.S., D.S., F.V.), University of Turin; Section of Human Anatomy, Department of Pharmacological Sciences and Experimental Medicine (S.K.T., F.A), University of Camerino, Italy.

Correspondence to Francesco Amenta, MD, Dipartimento di Scienze Farmacologiche e Medicina Sperimentale, Via Scalzino, 3-62032 Camerino, Italy. E-mail amenta{at}cambio.unicam.it

Abstract—We investigated the expression of {alpha}1-adrenergic receptor subtypes in intact human peripheral blood lymphocytes using reverse transcription–polymerase chain reaction (RT-PCR) and radioligand binding assay techniques combined with antibodies against the three subtypes of {alpha}1-adrenergic receptors ({alpha}1A, {alpha}1B, and {alpha}1D). RT-PCR amplified in peripheral blood lymphocytes a 348-bp {alpha}1A-adrenergic receptor fragment, a 689-bp {alpha}1B-adrenergic receptor fragment, and a 540-bp {alpha}1D-adrenergic receptor fragment. Radioligand binding assay with [3H]prazosin as radioligand revealed a high-affinity binding with a dissociation constant value of 0.65±0.05 nmol/L and a maximum density of binding sites of 175.3±20.5 fmol/106 cells. The pharmacological profile of [3H]prazosin binding to human peripheral blood lymphocytes was consistent with the labeling of {alpha}1-adrenergic receptors. Antibodies against {alpha}1A-, {alpha}1B-, and {alpha}1D-receptor subtypes decreased [3H]prazosin binding to a different extent. This indicates that human peripheral blood lymphocytes express the three {alpha}1-adrenergic receptor subtypes. Of the three different {alpha}1-adrenergic receptor subtypes, the {alpha}1B is the most represented and the {alpha}1D, the least. Future studies should clarify the functional relevance of {alpha}1-adrenergic receptors expressed by peripheral blood lymphocytes. The identification of these sites may represent a step for evaluating whether they represent a marker of {alpha}1-adrenergic receptors in cardiovascular disorders or for assessing responses to drug treatment on these receptors.


Key Words: lymphocytes • receptors, adrenergic, alpha • receptor subtypes • receptor antibodies




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