This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Belloni, A. S. Articles by Nussdorfer, G. G. Search for Related Content PubMed PubMed Citation Articles by Belloni, A. S. Articles by Nussdorfer, G. G. Related Collections Cell biology/structural biology Hypertension - basic studies Ion channels/membrane transport

(Hypertension. 1999;33:1185-1189.)
© 1999 American Heart Association, Inc.

Scientific Contributions

Proadrenomedullin N-Terminal 20 Peptide (PAMP), Acting Through PAMP(12–20)-Sensitive Receptors, Inhibits Ca²⁺-Dependent, Agonist-Stimulated Secretion of Human Adrenal Glands

Anna S. Belloni; Gian Paolo Rossi; Paola G. Andreis; Francesco Aragona; Hunter C. Champion; Philip J. Kadowitz; William A. Murphy; David H. Coy; Gastone G. Nussdorfer

From the Departments of Anatomy (A.S.B., P.G.A., G.G.N.), Clinical and Experimental Medicine (G.P.R.), and Urology (F.A.), School of Medicine, University of Padua, Padua, Italy; and the Departments of Pharmacology (H.C.C., P.J.K.) and Medicine (W.A.M., D.H.C.), School of Medicine, Tulane University, New Orleans, La.

Correspondence to Professor Gastone G. Nussdorfer, Department of Anatomy, Via Gabelli 65, I-35121 Padova, Italy. E-mail ggnanat{at}ipdunidx.unipd.it

Abstract—Proadrenomedullin N-terminal 20 peptide (PAMP) is a 20–amino acid hypotensive peptide expressed in the adrenal medulla. We investigated the localization and function of PAMP receptors in the human adrenal gland. Autoradiography showed the presence of [¹²⁵I]PAMP-binding sites in both zona glomerulosa and adrenal medulla that were displaced by cold PAMP and PAMP(12–20) but not by other preproadrenomedullin-derived peptides. PAMP, but not PAMP(12–20), counteracted, in a concentration dependent manner, both aldosterone response of zona glomerulosa cells and catecholamine response of adrenal medulla cells to BAYK-8644, the selective agonist of voltage-activated Ca²⁺ channels, as well as to K⁺ and angiotensin II. PAMP(12–20) partially reversed this antisecretagogue effect of PAMP. Collectively, these findings suggest (1) that PAMP inhibits Ca²⁺-dependent, agonist-stimulated aldosterone and catecholamine secretion, acting via specific receptors and through a mechanism involving the impairment of Ca²⁺ influx; and (2) that PAMP(12–20) acts as a weak antagonist of PAMP receptors, thereby suggesting that both C- and N-terminal sequences of the PAMP molecule are required for this peptide to exert its antisecretagogue action on the human adrenal gland.

Key Words: PAMP • adrenal glands • aldosterone • catecholamines

This article has been cited by other articles:

				D. Ribatti, M. T. Conconi, and G. G. Nussdorfer Nonclassic Endogenous Novel Regulators of Angiogenesis Pharmacol. Rev., June 1, 2007; 59(2): 185 - 205. [Abstract] [Full Text] [PDF]

				A. Martinez, J. A. Bengoechea, and F. Cuttitta Molecular Evolution of Proadrenomedullin N-Terminal 20 Peptide (PAMP): Evidence for Gene Co-Option Endocrinology, July 1, 2006; 147(7): 3457 - 3461. [Abstract] [Full Text] [PDF]

				A. Martinez, E. Zudaire, S. Portal-Nunez, L. Guedez, S. K. Libutti, W. G. Stetler-Stevenson, and F. Cuttitta Proadrenomedullin NH2-Terminal 20 Peptide Is a Potent Angiogenic Factor, and Its Inhibition Results in Reduction of Tumor Growth Cancer Res., September 15, 2004; 64(18): 6489 - 6494. [Abstract] [Full Text] [PDF]

				H. C. Champion, T. J. Bivalacqua, R. L. Pierce, W. A. Murphy, D. H. Coy, A. L. Hyman, and P. J. Kadowitz Responses to human CGRP, ADM, and PAMP in human thymic arteries Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2003; 284(2): R531 - R537. [Abstract] [Full Text] [PDF]

				P. G. Andreis, A. Markowska, H. C. Champion, G. Mazzocchi, L. K. Malendowicz, and G. G. Nussdorfer Adrenomedullin Enhances Cell Proliferation and Deoxyribonucleic Acid Synthesis in Rat Adrenal Zona Glomerulosa: Receptor Subtype Involved and Signaling Mechanism Endocrinology, June 1, 2000; 141(6): 2098 - 2104. [Abstract] [Full Text] [PDF]