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(Hypertension. 1999;34:872-875.)
© 1999 American Heart Association, Inc.

Scientific Contributions

Identification of Diadenosine Hexaphosphate in Human Erythrocytes

Jiankai Luo; Joachim Jankowski; Martin Tepel; Markus von der Giet; Walter Zidek; Hartmut Schlüter

From Medizinische Klinik I, Universitäts-Klinik Marienhospital, University of Bochum, Herne, Germany.

Correspondence to Dr Hartmut Schlüter, Medizinische Klinik I, Universitäts-Klinik Marienhospital, der Ruhr-Universität Bochum, Hölkeskampring 40, 44625 Herne, Germany. E-mail Hartmut.Schlueter{at}ruhr-uni-bochum.de

Abstract—Diadenosine polyphosphates have been identified as important regulators of vascular tone and blood pressure. In reference to the background of the well-known vasoconstriction induced by hemolysate, we questioned whether this action may be due in part to the presence of diadenosine polyphosphates in human erythrocytes. Therefore, erythrocytes were separated from other blood cells and deproteinated. To concentrate and purify nucleotides, the extract was chromatographed by anion exchange, affinity, and reversed-phase columns. In one of the purified fractions, diadenosine hexaphosphate (diadenosine 5', 5'-P₁, P₆ hexaphosphate [AP₆A]) was identified by matrix-assisted laser desorption/ionization mass spectrometry, ultraviolet spectroscopy, and enzymatic analysis. Hemolysate of erythrocytes injected into the isolated perfused rat kidney induced a dose-dependent vasoconstriction, which was partially inhibited by P₂-purinoceptor antagonist. The data document the existence of AP₆A in erythrocytes. AP₆A may be involved in the pathogenesis of vasospasm induced by free hemoglobin.

Key Words: diadenosine polyphosphates • erythrocytes • vasoactivity

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