This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ma, G. Articles by Young, D. B. Search for Related Content PubMed PubMed Citation Articles by Ma, G. Articles by Young, D. B. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB POTASSIUM Related Collections Primary prevention Secondary prevention Restenosis Cerebrovascular disease/stroke Catheter-based coronary and valvular interventions: other Catheter-based coronary interventions: stents

(Hypertension. 2000;35:948.)
© 2000 American Heart Association, Inc.

Scientific Contributions

Inhibition of Vascular Smooth Muscle Cell Migration by Elevation of Extracellular Potassium Concentration

Ge Ma; David P. Mason; David B. Young

From the Department of Physiology and Biophysics (G.M., D.B.Y.), University of Mississippi Medical Center (Jackson); and Department of Surgery (D.P.M.), University of Washington School of Medicine, Seattle.

Correspondence to David B. Young, PhD, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216-4505. E-mail mag{at}gwgate.nhlbi.nih.gov

Abstract—The effect of potassium on the migration of vascular smooth muscle cells was analyzed in media made with extracellular potassium concentrations of 3, 4, 5, and 6 mmol/L. The migration of cultured porcine coronary artery cells was stimulated with platelet-derived growth factor (PDGF)-BB. In the first study, cells were exposed to PDGF-BB at concentrations of 0, 10, or 20 ng/mL for 5 hours with the use of a Boyden chamber. Cells were quiescent overnight in 0.5% fetal bovine serum in Dulbecco’s modified Eagle’s medium with an extracellular potassium concentration of 4 mmol/L. With increasing potassium concentration, migration was significantly inhibited (P<0.02, 2-way ANOVA). In the cells exposed to 10 ng/mL PDGF-BB, migration ranged from 500±86% to 294±44% (value in wells with 0 ng/mL PDGF-BB and 4 mmol/L potassium concentration=100%) in medium containing 3 to 6 mmol/L extracellular potassium concentration (P<0.03). Long-term potassium exposure was investigated in cells grown in 5% serum in Dulbecco’s modified Eagle’s medium with an extracellular potassium concentration of 3, 4, 5, or 6 mmol/L for 3 to 4 weeks. Migration was assessed with 0 or 20 ng/mL PDGF-BB. Migration was significantly inhibited by the elevation of extracellular potassium concentration (P<0.01, 2-way ANOVA). With 20 ng/mL PDGF-BB, the migration rates ranged from 152±11% in medium with 3 mmol/L potassium to 69±5% in 6 mmol/L potassium (P<0.01). Increases in extracellular potassium concentration within the physiological range significantly and directly inhibit vascular smooth muscle cell migration.

Key Words: arteries • atherosclerosis • neointima • platelet-derived growth factor

This article has been cited by other articles:

				M. Kido, K. Ando, M. L. Onozato, A. Tojo, M. Yoshikawa, T. Ogita, and T. Fujita Protective Effect of Dietary Potassium Against Vascular Injury in Salt-Sensitive Hypertension Hypertension, February 1, 2008; 51(2): 225 - 231. [Abstract] [Full Text] [PDF]