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(Hypertension. 2000;36:159.)
© 2000 American Heart Association, Inc.

Scientific Contributions

Definitive Molecular Evidence of Renin-Angiotensin System in Human Uterine Decidual Cells

Chunbiao Li; Rais Ansari; Ziming Yu; Dinesh Shah

From the Department of Reproductive Biology, Case Western Reserve University, Cleveland, Ohio.

Correspondence to Dinesh Shah, MD, Case Western Reserve University, Maternal-Fetal Medicine, University MacDonald Womens Hospital, 11100 Euclid Ave, Suite 7008, Cleveland, OH 44106. E-mail dms23{at}cwru.edu

Abstract—The tissue renin-angiotensin system (RAS) has been suggested to be present in human gestational tissues, but uncertainty exists about the authenticity of this RAS, and the cellular origin of this RAS has not been defined. In the present study, we confirmed the presence of authentic renin and angiotensinogen in the prolactin-producing decidual tissue by sequencing the cDNAs generated through reverse transcription–polymerase chain reaction, confirming cDNA product sizes, and by performing Northern blot analysis of the RNA. Our comparative data demonstrate that prolactin has the highest expression in the decidual tissue, followed by renin, and that angiotensinogen has the least expression. We demonstrated with fluorescent in situ hybridization that prolactin-expressing endocrine decidual cells are the same cells that express both renin and angiotensinogen. These results have implications in regard to how the decidual RAS may be regulated and what potential role this local RAS may have in the pathogenesis of preeclampsia.

Key Words: renin-angiotensin system • human • pregnancy • preeclampsia

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