(Hypertension. 2000;36:617.)
© 2000 American Heart Association, Inc.
Scientific Contributions |
Angiotensin II Stimulates Extracellular Signal–Regulated Kinase Activity in Intact Pressurized Rat Mesenteric Resistance Arteries
From the Department of Pharmacology (K.M., Y.E.G.E.-H., M.J.M.), University of Aarhus, Aarhus, Denmark; INSERM Unit 141 (D.H., B.I.L., A.T.), Hôpital Lariboisière, Paris, France; and Franz-Volhard-Clinic (A.F.), Berlin, Germany.
Correspondence to M.J. Mulvany, Professor, Department of Pharmacology, University Park 240, University of Aarhus, DK-8000 Aarhus C, Denmark. E-mail mm{at}farm.au.dk
Abstract—The activation of extracellular signal–regulated kinases 1/2 (ERK1/2) was assessed in isolated rat mesenteric resistance arteries (200-µm diameter) in a pressure myograph and stimulated for 5 minutes by angiotensin II (Ang II, 0.1 µmol/L) with a pressure of 70 mm Hg. ERK1/2 activity was measured by using an in-gel assay, and ERK1/2 phosphorylation was measured by Western blot analysis with use of a phospho-specific ERK1/2 antibody. Ang II (0.1 µmol/L) induced contraction (28% of phenylephrine contraction, 10 µmol/L). ERK kinase inhibitor PD98059 (10 µmol/L) attenuated this contraction by 36% but not that to phenylephrine or K+ (60 mmol/L). In unpressurized arteries, Ang II increased ERK1/2 activity by 26%, and pressure (70 mm Hg) itself increased ERK1/2 activity by 72%. Ang II and pressure together acted synergistically, increasing ERK1/2 activity by 264%. Thus, in pressurized vessels, Ang II (0.1 µmol/L) increased ERK1/2 activity by 112%, calculated as [(364/172)-1]x100, which was confirmed by a measured 72% increase in ERK1/2 phosphorylation. Ang II type 1 receptor blockade by candesartan (10 µmol/L) abolished the Ang II–induced increase in ERK1/2 activity, but Ang II type 2 receptor blockade (PD123319, 10 µmol/L) did not. The Ang II–induced increase in ERK1/2 activity was inhibited by protein kinase C inhibitors Ro-31-8220 (1 µmol/L) and Go-6976 (300 nmol/L) and tyrosine kinase inhibitors genistein (1 µmol/L, general) and herbimycin A (1 µmol/L, c-Src family). The present findings show for the first time in intact resistance arteries that ERK1/2 activation is rapidly regulated by Ang II, is synergistic with pressure, and is involved in contraction. The ERK1/2 signaling pathway apparently includes upstream protein kinase C and c-Src.
Key Words: angiotensin II • receptors, angiotensin II • protein kinases • mesenteric arteries • rats
This article has been cited by other articles:
 |
|
 |
|
  J. Su, P. A. Lucchesi, R. A. Gonzalez-Villalobos, D. I. Palen, B. M. Rezk, Y. Suzuki, H. A. Boulares, and K. Matrougui Role of Advanced Glycation End Products With Oxidative Stress in Resistance Artery Dysfunction in Type 2 Diabetic Mice Arterioscler Thromb Vasc Biol, August 1, 2008; 28(8): 1432 - 1438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Belmadani, M. Zerfaoui, H. A. Boulares, D. I. Palen, and K. Matrougui Microvessel vascular smooth muscle cells contribute to collagen type I deposition through ERK1/2 MAP kinase, {alpha}v{beta}3-integrin, and TGF-{beta}1 in response to ANG II and high glucose Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H69 - H76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Belmadani, D. I. Palen, R. A. Gonzalez-Villalobos, H. A. Boulares, and K. Matrougui Elevated Epidermal Growth Factor Receptor Phosphorylation Induces Resistance Artery Dysfunction in Diabetic db/db Mice Diabetes, June 1, 2008; 57(6): 1629 - 1637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Escano Jr., L. B. Keever, A. A. Gutweiler, and B. T. Andresen Angiotensin II Activates Extracellular Signal-Regulated Kinase Independently of Receptor Tyrosine Kinases in Renal Smooth Muscle Cells: Implications for Blood Pressure Regulation J. Pharmacol. Exp. Ther., January 1, 2008; 324(1): 34 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Dubroca, X. Loyer, K. Retailleau, G. Loirand, P. Pacaud, O. Feron, J.-L. Balligand, B. I. Levy, C. Heymes, and D. Henrion RhoA activation and interaction with Caveolin-1 are critical for pressure-induced myogenic tone in rat mesenteric resistance arteries Cardiovasc Res, January 1, 2007; 73(1): 190 - 197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Paravicini and R. M. Touyz Redox signaling in hypertension Cardiovasc Res, July 15, 2006; 71(2): 247 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ardanaz and P. J. Pagano Hydrogen peroxide as a paracrine vascular mediator: regulation and signaling leading to dysfunction. Experimental Biology and Medicine, March 1, 2006; 231(3): 237 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Benkirane, E. C. Viel, F. Amiri, and E. L. Schiffrin Peroxisome Proliferator-Activated Receptor {gamma} Regulates Angiotensin II-Stimulated Phosphatidylinositol 3-Kinase and Mitogen-Activated Protein Kinase in Blood Vessels In Vivo Hypertension, January 1, 2006; 47(1): 102 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. I. Palen, S. Belmadani, P. A. Lucchesi, and K. Matrougui Role of SHP-1, Kv.1.2, and cGMP in nitric oxide-induced ERK1/2 MAP kinase dephosphorylation in rat vascular smooth muscle cells Cardiovasc Res, November 1, 2005; 68(2): 268 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Jackson, L. Gao, and C. Zhu Mechanism of the Vascular Angiotensin II/{alpha}2-Adrenoceptor Interaction J. Pharmacol. Exp. Ther., September 1, 2005; 314(3): 1109 - 1116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wu, M.-A. Laplante, and J. de Champlain Cyclooxygenase-2 Inhibitors Attenuate Angiotensin II-Induced Oxidative Stress, Hypertension, and Cardiac Hypertrophy in Rats Hypertension, June 1, 2005; 45(6): 1139 - 1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Shiratsuchi and M. D. Basson Activation of p38 MAPK{alpha} by extracellular pressure mediates the stimulation of macrophage phagocytosis by pressure Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1083 - C1093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dubroca, D. You, B. I. Levy, L. Loufrani, and D. Henrion Involvement of RhoA/Rho Kinase Pathway in Myogenic Tone in the Rabbit Facial Vein Hypertension, May 1, 2005; 45(5): 974 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. H. Shah, J. A. Olivares-Reyes, and K. J. Catt The Protein Kinase C Inhibitor Go6976 [12-(2-Cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5H-indolo(2,3-a)pyrrolo(3,4-c)-carbazole] Potentiates Agonist-Induced Mitogen-Activated Protein Kinase Activation through Tyrosine Phosphorylation of the Epidermal Growth Factor Receptor Mol. Pharmacol., January 1, 2005; 67(1): 184 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Lucchesi, A. Sabri, S. Belmadani, and K. Matrougui Involvement of Metalloproteinases 2/9 in Epidermal Growth Factor Receptor Transactivation in Pressure-Induced Myogenic Tone in Mouse Mesenteric Resistance Arteries Circulation, December 7, 2004; 110(23): 3587 - 3593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shiratsuchi and M. D. Basson Extracellular pressure stimulates macrophage phagocytosis by inhibiting a pathway involving FAK and ERK Am J Physiol Cell Physiol, June 1, 2004; 286(6): C1358 - C1366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rocic, H. Jo, and P. A. Lucchesi A role for PYK2 in ANG II-dependent regulation of the PHAS-1-eIF4E complex by multiple signaling cascades in vascular smooth muscle Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1437 - C1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Spurrell, T. V. Murphy, and M. A. Hill Intraluminal pressure stimulates MAPK phosphorylation in arterioles: temporal dissociation from myogenic contractile response Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1764 - H1773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Hill, S. J. Potocnik, L. A. Martinez-Lemus, and G. A. Meininger Delayed arteriolar relaxation after prolonged agonist exposure: functional remodeling involving tyrosine phosphorylation Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H849 - H856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeidan, I. Nordstrom, S. Albinsson, U. Malmqvist, K. Sward, and P. Hellstrand Stretch-induced contractile differentiation of vascular smooth muscle: sensitivity to actin polymerization inhibitors Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1387 - C1396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. E.G. Eskildsen-Helmond and M. J. Mulvany Pressure-Induced Activation of Extracellular Signal-Regulated Kinase 1/2 in Small Arteries Hypertension, April 1, 2003; 41(4): 891 - 897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. M. Bird, L. Zhang, and R. R. Magness Possible mechanisms underlying pregnancy-induced changes in uterine artery endothelial function Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2003; 284(2): R245 - R258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Massett, Z. Ungvari, A. Csiszar, G. Kaley, and A. Koller Different roles of PKC and MAP kinases in arteriolar constrictions to pressure and agonists Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2282 - H2287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Schubert, V. U. Kalentchuk, and U. Krien Rho kinase inhibition partly weakens myogenic reactivity in rat small arteries by changing calcium sensitivity Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2288 - H2295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. T Bloedon, A R. Jeffcoat, W. Lopaczynski, M. J Schell, T. M Black, K. J Dix, B. F Thomas, C. Albright, M. G Busby, J. A Crowell, et al. Safety and pharmacokinetics of purified soy isoflavones: single-dose administration to postmenopausal women Am. J. Clinical Nutrition, November 1, 2002; 76(5): 1126 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Mulvany Small Artery Remodeling and Significance in the Development of Hypertension Physiology, June 1, 2002; 17(3): 105 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xiao and L. Zhang ERK MAP kinases regulate smooth muscle contraction in ovine uterine artery: effect of pregnancy Am J Physiol Heart Circ Physiol, January 1, 2002; 282(1): H292 - H300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M.A.C. Martens, B. Demeilliers, D. Girardot, C. Daigle, H. H. Dao, D. deBlois, and P. Moreau Vessel-Specific Stimulation of Protein Synthesis by Nitric Oxide Synthase Inhibition: Role of Extracellular Signal-Regulated Kinases 1/2 Hypertension, January 1, 2002; 39(1): 16 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Matrougui, L. B. Tanko, L. Loufrani, D. Gorny, B. I. Levy, A. Tedgui, and D. Henrion Involvement of Rho-Kinase and the Actin Filament Network in Angiotensin II-Induced Contraction and Extracellular Signal-Regulated Kinase Activity in Intact Rat Mesenteric Resistance Arteries Arterioscler Thromb Vasc Biol, August 1, 2001; 21(8): 1288 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Davis, X. Wu, T. R. Nurkiewicz, J. Kawasaki, G. E. Davis, M. A. Hill, and G. A. Meininger Integrins and mechanotransduction of the vascular myogenic response Am J Physiol Heart Circ Physiol, April 1, 2001; 280(4): H1427 - H1433. [Abstract] [Full Text] [PDF]
|
|