(Hypertension. 2001;37:386.)
© 2001 American Heart Association, Inc.
Scientific Contributions |
Renin Gene Transfer Restores Angiogenesis and Vascular Endothelial Growth Factor Expression in Dahl S Rats
From the Department of Physiology, Medical College of Wisconsin, Milwaukee.
Correspondence to Andrew S. Greene, PhD, Medical College of Wisconsin, 8701 Watertown Plank Rd, PO Box 26509, Milwaukee, WI 53226-0509. E-mail agreene{at}mcw.edu
In a previous study, we demonstrated that Dahl S rats (SS group) have low plasma renin activity, whereas transfer of a region of chromosome 13 containing the renin gene from Dahl R onto a congenic strain of Dahl SS/Jr/Hsd/MCW rats (S/renRR group) restores renin secretory responses. In the present study, we compared the angiogenic responses to electrical stimulation in the SS and S/renRR groups to explore the hypotheses that the renin-angiotensin system is involved in vascular endothelial growth factor (VEGF) expression and angiogenesis in skeletal muscle. Congenic SS and S/renRR rats fed a 0.4% or 4% salt diet were surgically prepared by chronic implantation of an electrical stimulator. Another group of S/renRR rats was treated with lisinopril 2 days before the surgery and throughout the stimulation protocol. The right tibialis anterior (TA) and extensor digitorum longus (EDL) were stimulated for 8 hours per day for 7 days. The contralateral muscles served as controls. Western blot analysis was performed to identify VEGF protein expression in these muscles. Electrical stimulation produced no change in vessel density of the SS group fed a 0.4% salt diet (change 5.50% and 8.14% for EDL and TA, respectively). Transfer of a region containing the renin gene restored the angiogenic response (change 16% and 30% for EDL and TA, respectively) despite a significantly higher blood pressure. Blockade of the renin-angiotensin system by lisinopril or high salt restored the responses observed in the SS group fed a low salt diet. In addition, increases in VEGF expression to electrical stimulation were observed only in the S/renRR group fed a low salt diet. These results suggest that renin gene transfer restores angiogenesis and VEGF expression in the skeletal muscle of Dahl S rats.
Key Words: blood vessels • muscle, skeletal • growth substances • renin-angiotensin system • chromosome 13
This article has been cited by other articles:
 |
|
 |
|
  M. M. de Resende and A. S. Greene Effect of ANG II on endothelial cell apoptosis and survival and its impact on skeletal muscle angiogenesis after electrical stimulation Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2814 - H2821. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Xu, N. Tomita, K. Ikeuchi, and Y. Ikada Recovery of Small-Sized Blood Vessels in Ischemic Bone Under Static Magnetic Field Evid. Based Complement. Altern. Med., March 1, 2007; 4(1): 59 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Petersen, D. H. Munzenmaier, and A. S. Greene Angiotensin II infusion restores stimulated angiogenesis in the skeletal muscle of rats on a high-salt diet Am J Physiol Heart Circ Physiol, July 1, 2006; 291(1): H114 - H120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Drenjancevic-Peric, S. A. Phillips, J. R. Falck, and J. H. Lombard Restoration of normal vascular relaxation mechanisms in cerebral arteries by chromosomal substitution in consomic SS.13BN rats Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H188 - H195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Drenjancevic-Peric and J. H. Lombard Reduced Angiotensin II and Oxidative Stress Contribute to Impaired Vasodilation in Dahl Salt-Sensitive Rats on Low-Salt Diet Hypertension, April 1, 2005; 45(4): 687 - 691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Drenjancevic-Peric and J. H. Lombard Introgression of chromosome 13 in Dahl salt-sensitive genetic background restores cerebral vascular relaxation Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H957 - H962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sarlos, B. Rizkalla, C. J. Moravski, Z. Cao, M. E. Cooper, and J. L. Wilkinson-Berka Retinal Angiogenesis Is Mediated by an Interaction between the Angiotensin Type 2 Receptor, VEGF, and Angiopoietin Am. J. Pathol., September 1, 2003; 163(3): 879 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Kelly, C. Hepper, L. L. Wu, A. J. Cox, and R. E. Gilbert Vascular endothelial growth factor expression and glomerular endothelial cell loss in the remnant kidney model Nephrol. Dial. Transplant., July 1, 2003; 18(7): 1286 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Van Gieson, W. L. Murfee, T. C. Skalak, and R. J. Price Enhanced Smooth Muscle Cell Coverage of Microvessels Exposed to Increased Hemodynamic Stresses In Vivo Circ. Res., May 2, 2003; 92(8): 929 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Mattot, L. Moons, F. Lupu, D. Chernavvsky, R. A. Gomez, D. Collen, and P. Carmeliet Loss of the VEGF164 and VEGF188 Isoforms Impairs Postnatal Glomerular Angiogenesis and Renal Arteriogenesis in Mice J. Am. Soc. Nephrol., June 1, 2002; 13(6): 1548 - 1560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Silvestre, R. Tamarat, T. Senbonmatsu, T. Icchiki, T. Ebrahimian, M. Iglarz, S. Besnard, M. Duriez, T. Inagami, and B. I. Levy Antiangiogenic Effect of Angiotensin II Type 2 Receptor in Ischemia-Induced Angiogenesis in Mice Hindlimb Circ. Res., May 31, 2002; 90(10): 1072 - 1079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Tamarat, J.-S. Silvestre, N. Kubis, J. Benessiano, M. Duriez, M. deGasparo, D. Henrion, and B. I. Levy Endothelial Nitric Oxide Synthase Lies Downstream From Angiotensin II-Induced Angiogenesis in Ischemic Hindlimb Hypertension, March 1, 2002; 39(3): 830 - 835. [Abstract] [Full Text] [PDF]
|
|