This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brands, M. W. Articles by Fitzgerald, S. M. Search for Related Content PubMed PubMed Citation Articles by Brands, M. W. Articles by Fitzgerald, S. M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB GLUCOSE POTASSIUM SODIUM Related Collections Animal models of human disease Hypertension - basic studies Glucose intolerance

(Hypertension. 2001;37:733.)
© 2001 American Heart Association, Inc.

Scientific Contributions

Long-Term Glucose Infusion Increases Arterial Pressure in Dogs With Cyclooxygenase-2 Inhibition

Michael W. Brands; Allison E. Hailman; Sharyn M. Fitzgerald

From the Medical College of Georgia and University of Mississippi Medical Center.

Correspondence to Michael W. Brands, PhD, Department of Physiology, Medical College of Georgia, 1120 15th St, Augusta, GA 30912-3000. E-mail mbrands{at}mail.mcg.edu

A series of studies has shown that long-term infusion of insulin and glucose does not increase mean arterial pressure (MAP) in dogs, but we have shown that the same infusion protocol or infusion of glucose alone increases arterial pressure in rats. This study tested the hypothesis that infusing glucose alone in dogs, with all insulin derived from endogenous secretion, would increase arterial pressure. Because fructose feeding in dogs also has been shown not to cause hypertension and because we have shown that prostaglandin production increases during insulin and glucose infusion, this study also tested whether prostaglandins prevent the pressor response in dogs. Dogs were instrumented and assigned in random crossover design to long-term cyclooxygenase-2 (COX-2) inhibition. After baseline measurements, glucose was infused in all dogs for 6 days (500 g/d IV). Plasma insulin increased 3- to 4-fold and blood glucose increased significantly in both groups. The MAP (measured 24 h/d) response in control dogs was variable but on average tended to increase, although not significantly. In the dogs with COX-2 inhibition, however, MAP increased significantly to a peak of 9±2 mm Hg and an average of 6±1 mm Hg above control. There was significant sodium and volume retention during glucose infusion and a significant increase in glomerular filtration rate, but there were no between-group differences. Plasma renin activity increased only in the control group. This is the first study to report a long-term pressor response with glucose infusion and hyperinsulinemia in dogs, and it suggests that the inability to detect this relationship previously was due to prostaglandins.

Key Words: insulin • hypertension • prostaglandins • COX-2 • renin-angiotensin

This article has been cited by other articles:

				M. W. Brands, T. D. Bell, N. A. Rodriquez, P. Polavarapu, and D. Panteleyev Chronic glucose infusion causes sustained increases in tubular sodium reabsorption and renal blood flow in dogs Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2009; 296(2): R265 - R271. [Abstract] [Full Text] [PDF]

				Z. A. Radi Pathophysiology of Cyclooxygenase Inhibition in Animal Models Toxicol Pathol, January 1, 2009; 37(1): 34 - 46. [Abstract] [Full Text] [PDF]

				C.-C. Juan, Y.-W. Shen, Y. Chien, Y.-J. Lin, S.-F. Chang, and L.-T. Ho Insulin infusion induces endothelin-1-dependent hypertension in rats Am J Physiol Endocrinol Metab, November 1, 2004; 287(5): E948 - E954. [Abstract] [Full Text] [PDF]

				D. Song, E. Arikawa, D. Galipeau, M. Battell, and J. H. McNeill Androgens Are Necessary for the Development of Fructose-Induced Hypertension Hypertension, March 1, 2004; 43(3): 667 - 672. [Abstract] [Full Text] [PDF]

				M. Guazzi, G. Tumminello, M. Matturri, and M. D. Guazzi Insulin ameliorates exercise ventilatory efficiency and oxygen uptake in patients with heart failure-type 2 diabetes comorbidity J. Am. Coll. Cardiol., September 17, 2003; 42(6): 1044 - 1050. [Abstract] [Full Text] [PDF]