(Hypertension. 2001;38:249.)
© 2001 American Heart Association, Inc.
Scientific Contributions |
Increased Expression of Peroxisome Proliferator-Activated Receptor-
and -
in Blood Vessels of Spontaneously Hypertensive Rats
From the Multidisciplinary Research Group on Hypertension, Clinical Research Institute of Montreal, University of Montreal, Montreal, Quebec, Canada.
Correspondence to Ernesto L. Schiffrin, MD, PhD, FRCPC, Clinical Research Institute of Montreal, 110 Pine Ave W, Montreal, Quebec, Canada H2W 1R7. E-mail schiffe{at}ircm.qc.ca
Abstract— Peroxisome proliferator-activated receptors (PPARs) are a family of ligand-activated transcription factors that include PPAR-
, PPAR-
, and PPAR-
. We hypothesized that PPAR expression in blood vessels could be reduced in hypertension to result in increased vascular growth and reduced apoptosis. We investigated the abundance of PPAR- and PPAR- in aorta and mesenteric arteries from young (6-week-old) and adult (16-week-old) spontaneously hypertensive rats (SHR) compared with age-matched control Wistar-Kyoto rats (WKY). mRNA levels of PPAR- and PPAR- were determined by reverse transcription-polymerase chain reaction. Protein expression was evaluated by Western blot and by immunohistochemistry. PPAR- was expressed in aortic and mesenteric vascular smooth muscle cells (VSMCs) from intact tissue and cultured cells. PPAR- was expressed in intact vascular tissue but was almost undetectable in cultured VSMCs. In mesenteric arteries from adult SHR, PPAR- and PPAR- mRNA levels were significantly greater than in WKY (P<0.05). In aorta, PPAR- mRNA was significantly (P<0.05) more abundant in adult (but not in young) SHR than in WKY, whereas there was no difference in PPAR- mRNA between WKY and SHR. PPAR- and PPAR- mRNA were greater in mesenteric arteries (P<0.05) in young and adult SHR than in WKY. Expression of PPAR- and PPAR- was similar in SHR and WKY in other tissues. In cultured mesenteric VSMCs, PPAR- mRNA was 3-fold higher in SHR than in WKY. Immunohistochemistry demonstrated that PPAR- resided constitutively in the cytoplasm in primary and low-passaged aortic and mesenteric VSMCs, whereas PPAR- was almost undetectable. Thus, aorta and mesenteric resistance arteries from SHR in the prehypertensive and the established phase of hypertension exhibit increased expression of both PPAR isoforms, whereas other tissues do not. Changes (increases) in PPAR expression may play a compensatory role in the remodeling of blood vessels in SHR.
Key Words: PPAR • aorta • arteries • muscle, smooth • remodeling
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