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(Hypertension. 2001;38:267.)
© 2001 American Heart Association, Inc.

Scientific Contributions

Divergent Biological Actions of Coronary Endothelial Nitric Oxide During Progression of Cardiac Hypertrophy

David J. Grieve; Philip A. MacCarthy; Nicholas P. Gall; Alison C. Cave; Ajay M. Shah

From the Department of Cardiology, Guy’s, King’s and St Thomas’ School of Medicine, King’s College London (Denmark Hill Campus), London, United Kingdom.

Correspondence to Prof Ajay Shah, Department of Cardiology, GKT School of Medicine, Bessemer Road, London SE5 9PJ, United Kingdom. E-mail ajay.shah{at}kcl.ac.uk

Abstract— Coronary endothelial NO synthase expression and NO bioactivity were investigated at sequential stages during the progression of left ventricular hypertrophy. Male guinea pigs underwent abdominal aortic banding or sham operation. Left ventricular contractile function was quantified in isolated ejecting hearts. Coronary endothelial and vasodilator function were assessed in isolated isovolumic hearts in response to boluses of bradykinin (0.001 to 10 µmol/L), substance P (0.01 to 100 µmol/L), diethylamine NONOate (DEA-NO) (0.1 to 1000 µmol/L), N^G-monomethyl-L-arginine monoacetate (L-NMMA) (10 mmol/L), and adenosine (10 mmol/L). At a stage of compensated left ventricular hypertrophy (3 weeks), left ventricular endothelial NO synthase protein expression was unaltered (Western blot and immunocytochemistry). Vasoconstriction in response to L-NMMA was increased in banded animals compared with sham-operated animals (13.8±2.1% versus 6.2±1.3%, n=10; P<0.05), but agonist- and DEA-NO-induced vasodilation was similar in the 2 groups. At a stage of decompensated left ventricular hypertrophy (8 to 10 weeks), left ventricular endothelial NO synthase protein expression was significantly lower in banded animals (on Western analysis: banded animals, 7.8±0.4 densitometric units; sham-operated animals, 12.2±1.7 densitometric units; n=5; P<0.05). At this time point, vasoconstriction in response to L-NMMA was similar in the 2 groups, but vasodilatation in response to bradykinin (30.9±2.4% versus 39.7±2.2%, n=10; P<0.05), DEA-NO (26.2±1.8% versus 34.6±1.8%, n=10; P<0.05), and adenosine (24.3±2.0% versus 35.7±2.0%, n=10; P<0.01) was attenuated in banded animals. These findings indicate that there is an increase in the basal activity of NO (without a significant change in endothelial NO synthase expression) in early compensated left ventricular hypertrophy, followed by a decrease in both endothelial NO synthase expression and NO bioactivity during the transition to myocardial failure.

Key Words: hypertrophy • pressure overload • nitric oxide • arteries • endothelium • nitric oxide synthase

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