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(Hypertension. 2002;39:229.)
© 2002 American Heart Association, Inc.

Scientific Contributions

Tonin and Kallikrein in the Brain of Transgenic Rat Line Expressing Human Tissue Kallikrein

Eliane S.L. Lomez; Ronaldo C. Araujo; Michael Bader; Joao B. Pesquero; Jorge L. Pesquero

From the Departments of Physiology and Biophysics, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais (E.S.L.L., J.L.P.), University of Mogi das Cruzes (R.C.A.), and Universidade Federal de Sao Paulo (J.B.P.), Brazil; and Molecular Biology of Peptides Hormones, Max-Delbruck Center for Molecular Medicine (M.B.), Berlin-Buch, Germany.

Corresponding author Dr Jorge Luiz Pesquero, Depto Fisiologia e Biofisica, ICB, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, 31270-901, Belo Horizonte, MG, Brazil. E-mail jlpesq{at}icb.ufmg.br

A transgenic rat line harboring the human tissue kallikrein gene was investigated for expression and activity of tonin and kallikrein in different regions of the brain. The introduction of the transgene into the rat genome produced a significant augmentation of the expression levels and activity of rat tissue kallikrein. The possibility that human kallikrein does not hydrolyze the rat substrate is probably responsible for the augmented expression of the rat enzyme. On the other hand, although expression of tonin was significantly reduced, tonin activity was not altered in most brain structures, except for cerebellum and neurohypophysis.

Key Words: tonin • kallikrein • angiotensin II • rats • brain

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