This Article Full Text Full Text (PDF) All Versions of this Article: 42/4/523    most recent 01.HYP.0000093383.18302.A7v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rong, P. Articles by Skinner, S. L. Search for Related Content PubMed PubMed Citation Articles by Rong, P. Articles by Skinner, S. L. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information High Blood Pressure Related Collections Animal models of human disease Energy metabolism

(Hypertension. 2003;42:523.)
© 2003 American Heart Association, Inc.

Scientific Contributions

Hypertension in the (mRen-2)27 Rat Is Not Explained by Enhanced Kinetics of Transgenic Ren-2 Renin

From the Department of Physiology (P.R., S.L.S.) and St Vincent’s Institute of Medical Research and Department of Medicine (D.J.C), University of Melbourne, Melbourne, Australia.

Correspondence to Sandford L. Skinner, MD, Department of Physiology, University of Melbourne, Victoria 3010, Australia. E-mail s.skinner{at}physiology.unimelb.edu.au

Enhanced efficiency of the reaction between transgenic Ren-2 mouse renin and endogenous rat angiotensinogen has been suggested as 1 mechanism that contributes to the accelerated hypertension and increased tissue angiotensin of the (mRen-2)27 transgenic rat. This was tested in a study conducted at pH 7.4 in vitro that compared the kinetic constants of purified mouse Ren-2 and rat renin (each at 100, 75, 50, and 25 pmol/L) reacting with physiologic concentrations of rat angiotensinogen (0 to 4 µmol/L). Under these conditions, the kinetic constants for Ren-2 (K_m, 1.8 µmol/L; K_cat, 0.07/s; and K_cat/K_m, 0.04 L · µmol^-1 · s^-1) were not different from rat renin. However, Ren-2 renin acting on its homologous mouse angiotensinogen was confirmed as being much slower. We conclude that hypertension in the Ren-2 rat is not related to renin kinetics. Other mechanisms are considered, with reference to human essential hypertension.

Key Words: renin-angiotensin system • angiotensinogen • blood pressure • hypertension, essential

This article has been cited by other articles:

				D. J. Campbell, H. Karam, J. Menard, P. Bruneval, and J. J. Mullins Prorenin Contributes to Angiotensin Peptide Formation in Transgenic Rats With Rat Prorenin Expression Targeted to the Liver Hypertension, December 1, 2009; 54(6): 1248 - 1253. [Abstract] [Full Text] [PDF]

				L. J. Mullins, M. A. Bailey, and J. J. Mullins Hypertension, Kidney, and Transgenics: A Fresh Perspective Physiol Rev, April 1, 2006; 86(2): 709 - 746. [Abstract] [Full Text] [PDF]