This Article Full Text Full Text (PDF) All Versions of this Article: 44/6/907    most recent 01.HYP.0000146400.57221.74v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhong, J.-C. Articles by Du, K. Search for Related Content PubMed PubMed Citation Articles by Zhong, J.-C. Articles by Du, K. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB TRANS-RETINOIC ACID Related Collections Remodeling Cardiovascular Pharmacology Animal models of human disease Risk Factors Physiological and pathological control of gene expression Oxidant stress

(Hypertension. 2004;44:907.)
© 2004 American Heart Association, Inc.

Scientific Contributions

Upregulation of Angiotensin-Converting Enzyme 2 by All-trans Retinoic Acid in Spontaneously Hypertensive Rats

Jiu-Chang Zhong; Dong-Yang Huang; Yan-Mei Yang; Yi-Fan Li; Ge-Fei Liu; Xu-Hong Song; Kun Du

From Molecular Biology Center, Shantou University Medical College, Shantou, China.

Correspondence to Dong-Yang Huang, MD, Molecular Biology Center, Shantou University Medical College, 22 Xin Ling Rd, Shantou, Guangdong 515041, China. E-mail huangdy{at}stu.edu.cn or g_jczhong@stu.edu.cn

There is increasing evidence that all-trans retinoic acid (atRA) influences gene expression of components of renin-angiotensin system (RAS), which plays a pivotal role in the pathophysiology of essential hypertension. To further validate effects of atRA on the RAS and to assess the possibility that atRA affects the activity of angiotensin-converting enzyme 2 (ACE2), gene, and protein expression of ACE2 have been examined by real-time polymerase chain reaction and Western blot methods in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. Rats were treated with atRA (10 or 20 mg · kg^–1 · day^–1) or placebo given as daily intraperitoneal injection for 1 month. ACE2 expression was markedly decreased in placebo-treated SHR when compared with WKY rats. However, in atRA-treated SHR, a significant upregulation of ACE2 expression was observed in heart and kidney. In conclusion, chronic atRA treatment increases gene and protein expressions of ACE2, resulting in the reduction of blood pressure and the attenuation of myocardial damage in SHR, which suggests that atRA may be an attractive candidate for the potential prevention and treatment of human essential hypertension.

Key Words: hypertension, essential • angiotensin-converting enzyme • renin-angiotensin system • rats

This article has been cited by other articles:

				A. Baraka, M. Mikhail, A. Guemei, and S. El Ghotny Effect of Targeting Mitogen-Activated Protein Kinase on Cardiac Remodeling in Rats Journal of Cardiovascular Pharmacology and Therapeutics, December 1, 2009; 14(4): 339 - 346. [Abstract] [PDF]

				H. Xia, Y. Feng, T. D. Obr, P. J. Hickman, and E. Lazartigues Angiotensin II Type 1 Receptor-Mediated Reduction of Angiotensin-Converting Enzyme 2 Activity in the Brain Impairs Baroreflex Function in Hypertensive Mice Hypertension, February 1, 2009; 53(2): 210 - 216. [Abstract] [Full Text] [PDF]

				A. Zulli, S. Rai, B. F. Buxton, L. M. Burrell, and D. L. Hare Co-localization of angiotensin-converting enzyme 2-, octomer-4- and CD34-positive cells in rabbit atherosclerotic plaques Exp Physiol, May 1, 2008; 93(5): 564 - 569. [Abstract] [Full Text] [PDF]

				H. Ji, S. Menini, W. Zheng, C. Pesce, X. Wu, and K. Sandberg Role of angiotensin-converting enzyme 2 and angiotensin(1-7) in 17{beta}-oestradiol regulation of renal pathology in renal wrap hypertension in rats Exp Physiol, May 1, 2008; 93(5): 648 - 657. [Abstract] [Full Text] [PDF]

				R. Choudhary, A. Palm-Leis, R. C. Scott III, R. S. Guleria, E. Rachut, K. M. Baker, and J. Pan All-trans retinoic acid prevents development of cardiac remodeling in aortic banded rats by inhibiting the renin-angiotensin system Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H633 - H644. [Abstract] [Full Text] [PDF]

				H. Kobori, M. Nangaku, L. G. Navar, and A. Nishiyama The Intrarenal Renin-Angiotensin System: From Physiology to the Pathobiology of Hypertension and Kidney Disease Pharmacol. Rev., September 1, 2007; 59(3): 251 - 287. [Abstract] [Full Text] [PDF]

				A. J. Trask, D. B. Averill, D. Ganten, M. C. Chappell, and C. M. Ferrario Primary role of angiotensin-converting enzyme-2 in cardiac production of angiotensin-(1-7) in transgenic Ren-2 hypertensive rats Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H3019 - H3024. [Abstract] [Full Text] [PDF]

				J.-C. Zhong, X.-Y. Yu, Y. Huang, L.-M. Yung, C.-W. Lau, and S.-G. Lin Apelin modulates aortic vascular tone via endothelial nitric oxide synthase phosphorylation pathway in diabetic mice Cardiovasc Res, June 1, 2007; 74(3): 388 - 395. [Abstract] [Full Text] [PDF]

				C. Diez-Freire, J. Vazquez, M. F. Correa de Adjounian, M. F. R. Ferrari, L. Yuan, X. Silver, R. Torres, and M. K. Raizada ACE2 gene transfer attenuates hypertension-linked pathophysiological changes in the SHR Physiol Genomics, January 12, 2007; 27(1): 12 - 19. [Abstract] [Full Text] [PDF]

				S. A. R. Paiva, L. S. Matsubara, B. B. Matsubara, M. F. Minicucci, P. S. Azevedo, A. O. Campana, and L. A. M. Zornoff Retinoic Acid Supplementation Attenuates Ventricular Remodeling after Myocardial Infarction in Rats J. Nutr., October 1, 2005; 135(10): 2326 - 2328. [Abstract] [Full Text] [PDF]