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(Hypertension. 2005;46:51.)
© 2005 American Heart Association, Inc.

Original Articles

Role of Calcitonin Gene-Related Peptide in Hypertension-Induced Renal Damage

Mark C. Bowers; Khurshed A. Katki; Arundhati Rao; Michael Koehler; Parag Patel; Alvin Spiekerman; Donald J. DiPette; Scott C. Supowit

From Texas A&M University System Health Science Center, College of Medicine and Scott & White Health System, Department of Medicine (M.C.B., K.A.K., M.K., D.J.D., S.C.S.), Temple, Tex; Scott & White Health System (A.R., P.P., A.S.), Department of Pathology, Temple, Tex.

Correspondence to Scott Supowit, Texas A&M University Medical Research Building, 702 SW H.K. Dodgen Loop, Temple, TX 76504. E-mail ssupowit{at}swmail.sw.org

Calcitonin gene-related peptide, a potent vasodilator neuropeptide, is localized in perivascular sensory nerves. We have reported that -calcitonin gene-related peptide knockout mice have elevated baseline blood pressure and enhanced hypertension-induced renal damage compared with wild-type controls. Thus, the aim of this study was to determine the mechanism and functional significance of this increased hypertension-induced renal damage. We previously demonstrated by telemetric recording that the deoxycorticosterone–salt protocol produces a 35% increase in mean arterial pressure in both -calcitonin gene-related peptide knockout and wild-type mice. Both strains of mice were studied at 0, 14, and 21 days after deoxycorticosterone–salt hypertension. Renal sections from hypertensive wild-type mice showed no pathological changes at any time point studied. However, on days 14 and 21, hypertensive knockout mice displayed progressive increases in glomerular proliferation, crescent formation, and tubular protein casts, as well as the inflammatory markers intercellular adhesion molecule-1, vascular adhesion molecule-1, and monocyte chemoattractant protein-1. There was a significant increase in 24-hour urinary isoprostane, a marker of oxidative stress-induced lipid peroxidation, levels at days 14 and 21 in the hypertensive knockout compared with hypertensive wild-type mice. Urinary microalbumin was significantly higher (2-fold) at day 21 and creatinine clearance was significantly decreased 4-fold in the hypertensive knockout compared with hypertensive wild-type mice. Therefore, in the absence of -calcitonin gene-related peptide, deoxycorticosterone–salt hypertension induces enhanced oxidative stress, inflammation, and renal histopathologic damage, resulting in reduced renal function. Thus, sensory nerves, via -calcitonin gene-related peptide, appear to be renoprotective against hypertension-induced damage.

Key Words: calcitonin gene-related peptide • hypertension • kidney

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