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(Hypertension. 2008;51:899.)
© 2008 American Heart Association, Inc.

Original Articles

Renal Regional Proteomes in Young Dahl Salt-Sensitive Rats

Zhongmin Tian; Andrew S. Greene; Kristie Usa; Isaac R. Matus; Jesse Bauwens; Jennifer L. Pietrusz; Allen W. Cowley, Jr; Mingyu Liang

From the Department of Physiology (Z.T., A.S.G., K.U., J.B., J.L.P., A.W.C., M.L.), Biotechnology and Biomedical Engineering Center (Z.T., A.S.G., I.R.M.), Medical College of Wisconsin, Milwaukee; and the Department of Biomedical Engineering (Z.T.), Xi’an Jiaotong University, Xi’an, Shanxi, People’s Republic of China.

Correspondence to Mingyu Liang, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226. E-mail mliang{at}mcw.edu

We performed an extensive proteomic analysis of the Dahl model of salt-sensitive hypertension. The consomic SS-13^BN rat, genetically similar to the Dahl salt-sensitive rat, while exhibiting a significant amelioration of salt-induced hypertension, was used as a control. Proteomic analysis, using differential in-gel electrophoresis and mass spectrometry techniques, was performed in the renal cortex and the renal medulla of 6-week–old SS and SS-13^BN rats before significant differences in blood pressure were developed between the 2 strains of rat. Several dozen proteins were identified as differentially expressed between SS and SS-13^BN rats fed the 0.4% NaCl diet or switched to the 4% NaCl diet for 3 days (n=4). The identified proteins were involved in cellular functions or structures including signal transduction, energy metabolism, and the cytoskeleton. The proteomic analysis and subsequent Western blotting indicated that heterogeneous nuclear ribonucleoprotein K in the renal medulla was upregulated by the 4% NaCl diet in SS-13^BN rats but downregulated in SS rats. The level of angiotensinogen mRNA in the renal medulla was regulated in an opposite manner. Silencing of heterogeneous nuclear ribonucleoprotein K resulted in an upregulation of angiotensinogen in cultured human kidney cells. In summary, we identified significant differences in kidney regional proteomic profiles between SS and SS-13^BN rats and demonstrated a potential role of heterogeneous nuclear ribonucleoprotein K in the regulation of angiotensinogen expression in the renal medulla.

Key Words: hypertension • gene • proteomics • kidney • angiotensin

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