on February 19, 2008
Published online before print February 19, 2008, doi: 10.1161/HYPERTENSIONAHA.107.108415
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Submitted on December 5, 2007
From the Hubrecht Institute, Utrecht, The Netherlands. * To whom correspondence should be addressed. E-mail: e.cuppen{at}niob.knaw.nl.
Abstract—The existence of the A1079T transversion in the
Revised on December 23, 2007
The Atp1a1 Gene From Inbred Dahl Salt Sensitive Rats Does Not Contain the A1079T Missense Transversion
Michal Mokry and Edwin Cuppen*
1 isoform of the Na+, K+-ATPase (Atp1a1) gene in Dahl salt-sensitive rat (SS/Jr) strain, discovered by Herrera and Ruiz-Opazo and proposed to underlay hypertension sensitivity, represents one of the most controversial topics in hypertension research. As our research group did not have any previous connection to any party in this dispute nor to hypertension-related research, we were asked (J Hypertens. 2006;24:2312–2313) to definitively adjudge the existence of the A1079T transversion. Hence, different state-of-the art SNP detection technologies that depend on a variety of mechanisms and enzymes to detect the transversion in genomic DNA as well as cDNA derived from different tissues were used. Although it was possible to readily detect other silent polymorphisms between SS and SR strains in the Atp1a1 gene by all methods used, no evidence for the existence of the A1079T transversion in SS/Jr rats was found.
Key words: hypertension • inbred Dahl rats • missense mutation • sodium-potassium–exchanging ATPase • genetic polymorphism