Enalapril (E) Attenuates Oxidative Stress (OXS) in Diabetic Rats.
Oxidative stress has been involved as a possible mechanism of tissue injury. The effect of E on OxS and tissue injury was studied in kidney and heart from streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley, were divided into: STZ (65 mg/kg, single i.p. dose); STZ+E (STZ and E, 20 mg E /L drinking water), and Control (C) (saline i.p. and tap water). At 6 mo. organs were studied by light microscopy. Glomerular (GML) and tubulointerstitial lesions (TIL) were graded by a semiquantitative score (0-4). Glycemia, creatinine clearance (CrCl) and proteinuria were determined. OxS was evaluated by determining total glutathione content (GSH), protein-associated sulfhydryls (SH) and 2-thiobarbituric reactive substances (TBARS, an indicator of lipid oxidation) in kidney and heart homogenates. In STZ rats E decreased proteinuria, GML and TIL,and increased CrCl. In kidney and heart, E attenuated OxS associated to STZ. Results suggest that, in addition to previously described mechanisms, E might protect renal and cardiac tissue by attenuating OxS in STZ-induced diabetic rats.